Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats

Sousa, Luane Macêdo de; Alves, Joana Maria dos Santos; Martins, Conceição da Silva; Pereira, Karuza Maria Alves; Góes, Paula; Gondim, Delane Viana

doi:10.1007/s00011-019-01274-4

Cited by 18 publications

(24 citation statements)

References 54 publications

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“…The Wnt/β-catenin signaling pathway is a classical Wnt signaling pathway, and it is also the most detailed and important Wnt signaling pathway currently studied. The Wnt/β-catenin pathway can affect the process and prognosis of RA by affecting the proliferation of fibroblast-like synoviocytes and regulating the secretion of inflammatory factors and bone metabolism/bone destruction [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Crocin Alleviates Pain Hyperalgesia in AIA Rats by Inhibiting the Spinal Wnt5a/β-Catenin Signaling Pathway and Glial Activation

Wang

et al. 2020

Neural Plasticity

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At present, most of the drugs have little effect on the pathological process of rheumatoid arthritis (RA). Analgesia is an important measure in the treatment of RA and is also one of the criteria to determine the therapeutic effects of the disease. Some studies have found that crocin, a kind of Chinese medicine, can effectively alleviate pain sensitization in pain model rats, but the mechanism is not clear. Emerging evidence indicates that crocin may inhibit the metastasis of lung and liver cancer cells from the breast by inhibiting Wnt/β-catenin and the Wnt signaling pathway is closely related to RA. Wnt5a belongs to the Wnt protein family and was previously thought to be involved only in nonclassical Wnt signaling pathways. Recent studies have shown that Wnt5a has both stimulatory and inhibitory effects on the classical Wnt signaling pathway, and so, Wnt5a has attracted increasing attention. This study demonstrated that crocin significantly increased the mechanical thresholds of adjuvant-induced arthritis (AIA) rats, suggesting that crocin can alleviate neuropathic pain. Crocin significantly decreased the levels of pain-related factors and glial activation. Foxy5, activator of Wnt5a, inhibited the above effects of crocin in AIA rats. In addition, intrathecal injection of a Wnt5a inhibitor significantly decreased hyperalgesia in AIA rats. This research shows that crocin may alleviate neuropathic pain in AIA rats by inhibiting the expression of pain-related molecules through the Wnt5a/β-catenin pathway, elucidating the mechanism by which crocin relieves neuropathic pain and provides a new way of thinking for the treatment of AIA pain.

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Section: Introductionmentioning

confidence: 99%

Crocin Alleviates Pain Hyperalgesia in AIA Rats by Inhibiting the Spinal Wnt5a/β-Catenin Signaling Pathway and Glial Activation

Wang

et al. 2020

Neural Plasticity

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“…Related studies demonstrated that interaction of CRP with its receptor promoted proin ammatory cytokines (IL-1β, IL-6 and TNF-α) production, leading to ampli cation of in ammatory reaction [26,36]. And in CFA induced TMJ in ammation, IL-1β and TNF-α acted as the speci c up-regulated proin ammatory factors [11,12,37]. By PCR analysis and IHC staining in synovial membrane, we found lower level of IL-1β and IL-6 in CFA + CRP group compared to CFA group (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In ammation is believed to be the vital factor for this pathological process, since pro-in ammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukins IL-1β and IL-6 are elevated in synovial uid of TMD patients [6,10]. While up-regulated in ammation factors aggravate TMJ-OA development, medical treatments targeted on in ammatory cytokines and signaling pathways show bene t effects on in ammation absorption and TMJ-OA recovery [11,12]. TNF-α inhibitors and IL-1 receptor antagonist are the well-established therapeutic approaches for RA treatment [13][14][15], and antibodies to TNF-α, IL-1β and IL-6 show positive effects during RA therapy investigation [16,17].…”

Section: Introductionmentioning

confidence: 99%

C-Reactive Protein Knock Out Attenuate Temporomandibular Joint Inflammation in Rats

Zhou

Jian

et al. 2020

Preprint

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Background: C-reactive protein (CRP), as the biomarker for inflammation, high expresses in osteoarthritis (OA) related diseases with exact role been clouded. In this study, we evaluated the biological effect of CRP in temporomandibular joint osteoarthritis (TMJ-OA).Methods: Freund’s complete adjuvant (CFA) was used for TMJ inflammation induction in CRP knock out (CRP -/-) and control rats. And TMJ degenerative changes, such as the synovitis performance, TMJ disc morphological changes and cartilage degeneration were compared to elucidate the role of CRP played in TMJ-OA. Results: Compared to control group, CFA induced TMJ inflammation caused systemic and local CRP expression increased. Based on this, CRP -/- rat performed less severe inflammation symptoms. Lower degree of pro-inflammatory cytokines (interleukins IL-1β and IL-6) expression and up-expression of anti-inflammatory cytokine IL-10 were detected in CRP -/- rat, which with less macrophage and activation and osteoclast differentiation.Conclusion: These results indicated that control high elevated CRP level during inflammation should be benefit for TMJ-OA prevention and treatment.

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“…The OA symptoms are subchondral bone sclerosis; synovial inflammation includes synovial hyperplasia, fibrosis, and thickening of the synovial capsule, activated synoviocytes, and in some cases, lymphocytic infiltrate (B- and T-cells as well as plasma cells), cartilage degradation, ligament calcification, and osteophyte formation [ 3 ]. The pathogenesis of OA involves the interaction between mechanical, genetic, metabolic, and inflammatory mechanisms [ 4 , 5 ]. Traditional pharmacological therapies and nonpharmacological treatments such as surgical procedures can only provide symptomatic relief; however, damaged cartilage cannot be restored effectively [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study on the Effect of Exosomes Secreted by Mesenchymal Stem Cells Derived from Adipose and Bone Marrow Tissues in the Treatment of Osteoarthritis-Induced Mouse Model

Fazaeli

Kalhor

Naserpour

et al. 2021

BioMed Research International

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Background. Exosomes as extracellular vesicles (EVs) are nanoscale intercellular messengers secreted from cells to deliver biological signals. Today, exosomes have become a new field of research in regenerative medicine and are considered as potential therapies to control inflammation and wound healing and enhance and improve healing in many diseases. Given the global burden of osteoarthritis (OA) as the fastest-growing health condition and one of the major causes of physical disability in the aging population, research to establish EVs as therapeutic products can meet the basic clinical needs in the management of osteoarthritis and provide a therapeutic solution. Objectives. The present study is aimed at evaluating the regenerative potentials of the exosomes secreted from adipose and bone marrow tissue-derived mesenchymal stem cells (AD- and BM-MSCs) in ameliorating the symptoms of OA. Method. In this experimental study, AD- and BM-MSCs were isolated and cultured in the laboratory until passage 3. Finally, these cells’ secreted exosomes were isolated from their conditioned medium. Ciprofloxacin-induced OA mouse models underwent intra-articular injection of exosomes from AD-MSCs and BM-MSCs. Finally, the expression levels of collagen I and II, sox9, and aggrecan genes using real-time PCR, histological analysis, and immunohistochemical (IHC) studies were performed. Results. Real-time PCR data showed that although the expression level of collagen type II was lower in both exosome-treated groups than the normal, but it was significantly increased in comparison with the sham and OA, with higher expression in BM-Exo rather than AD-Exo group. Similarly, the histological staining and IHC results have provided almost identical data, emphasizing on better therapeutic effect of BM-MSCs-exosome than AD-MSCs-exosome. Conclusion. BM-MSCs secreted exosomes in comparison with AD-MSCs could be considered as a better therapeutic option to improve osteoarthritis and exhibit potential as a disease-modifying osteoarthritis cell-free product.

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Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats

Cited by 18 publications

References 54 publications

Crocin Alleviates Pain Hyperalgesia in AIA Rats by Inhibiting the Spinal Wnt5a/β-Catenin Signaling Pathway and Glial Activation

Crocin Alleviates Pain Hyperalgesia in AIA Rats by Inhibiting the Spinal Wnt5a/β-Catenin Signaling Pathway and Glial Activation

C-Reactive Protein Knock Out Attenuate Temporomandibular Joint Inflammation in Rats

A Comparative Study on the Effect of Exosomes Secreted by Mesenchymal Stem Cells Derived from Adipose and Bone Marrow Tissues in the Treatment of Osteoarthritis-Induced Mouse Model

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