Immunoexpression of lactoferrin in human sporadic renal cell carcinomas

Giuffrè, Giuseppe; Barresi, Valeria; Skliros, Christodoulos; Barresi, G; Tuccari, Giovanni

doi:10.3892/or.17.5.1021

Cited by 16 publications

(36 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since NGAL is involved in iron uptake, we may hypothesize that higher NGAL expression may reflect augmented iron requirement in some RCCs in comparison to others. That papillary and chromophobe histotypes may need higher iron uptake is also suggested by the higher expression of other iron-related proteins, such as lactoferrin (Lf), in these tumors in comparison to other renal neoplasias (35,36). Besides, increased iron transport to the inner cells may be at the basis of carcinogenesis of renal tumors.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil gelatinase-associated lipocalin immunoexpression in renal tumors: Correlation with histotype and histological grade

Barresi

Ieni²,

Bolignano³

et al. 2010

Oncol Rep

View full text Add to dashboard Cite

Abstract. Neutrophil gelatinase-associated lipocalin (NGAL) is a 25 kDa protein with roles in iron trafficking as well as in carcinogenesis and progression of several human neoplasias. Although the renal proximal tubule represents a major source of NGAL synthesis under various injurious stimuli to the kidney, NGAL expression has been rarely evaluated in renal tumors up to now. In view of this, in the present study we analyzed the expression of this protein in renal tumors of different histotype and grade so as to evaluate whether a role for NGAL might be also proposed in the carcinogenesis of these neoplasms. NGAL immunoexpression was analyzed in 30 surgically resected renal tumors [18 clear cell, 5 papillary and 3 chromophobe renal cell carcinomas (RCCs), 2 urothelial carcinomas and 2 oncocytomas] and in the peritoneal metastasis of a clear cell RCC. A variable NGAL immunoexpression was found in 28/30 cases. High NGAL expression was significantly associated with the papillary and chromphobe histotypes (P=0.016) and with a higher histological grade of clear cell and papillary RCC (P=0.004). Moreover, NGAL expression was retained in the peritoneal metastasis of clear cell RCC. Our findings demonstrate that NGAL is expressed in several histotypes of renal tumors. Its highest expression in the papillary and chromophobe histotypes might be related to a higher need in iron uptake, which could be exploited in anti-cancer therapies with iron chelators against these neoplasias. Further studies are required to investigate the potential diagnostic utility of NGAL in the early diagnosis of metastatic progression of RCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Neutrophil gelatinase-associated lipocalin immunoexpression in renal tumors: Correlation with histotype and histological grade

Barresi

Ieni²,

Bolignano³

et al. 2010

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…The present study retrospectively investigated LF immunoexpression in a cohort of 65 TNBC cases that were surgically treated by breast-conserving surgery (lumpectomy, quadrantectomy, partial mastectomy or segmental mastectomy), at the polyclinic 'G. Immunohistochemical procedures, and positive and negative controls of LF staining were performed as previously described (18,19).…”

Section: Methodsmentioning

confidence: 99%

“…Patients, n n (%) been considered a potential marker for glandular or acinar differentiation, similar to its use in other malignancies (9,18,22). However, in breast cancer, the association between LF expression and clinical parameters is not well defined (24), even if it has been hypothesized that tumors with high LF expression retain a certain degree of physiological control over cell growth, which may explain a good prognosis (25).…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

“…Lactoferrin (LF), an iron binding 78-to 80-kDa glycoprotein usually present in mammalian milk, has been immunohistochemically revealed in numerous human neoplastic conditions of different sites (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)). An immunopositive LF rate with a large variability, ranging from 7.5 to 42% of cases, has previously been identified in breast carcinoma (23)(24)(25)(26)(27); however, LF was more often observed in low-grade ER/PR-positive ductal carcinomas, confirming a decrease in LF immunostaining in less differentiated and more aggressive breast carcinomas (25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunoexpression of lactoferrin in triple-negative breast cancer patients: A proposal to select a less aggressive subgroup

et al. 2017

Self Cite

View full text Add to dashboard Cite

Abstract. Triple-negative breast cancer (TNBC) indicates a subset of breast carcinomas that does not express estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER2). According to the literature, TNBCs are aggressive tumors, characterized by a high incidence of recurrence and a high risk of disease progression. Lactoferrin (LF) is a single-chain, iron-binding glycoprotein of ~700 amino acids, which is involved in a wide range of biological activities, including iron-trafficking and carcinogenesis. The present study aimed to assess LF expression in human TNBC samples and the possible correlation with clinico-pathological parameters associated with biological aggressiveness. LF immunohistochemical expression was investigated in formalin-fixed, paraffin-embedded samples of human TNBC. Cases were analyzed according to an intensity distribution (ID) score, and only those showing an ID score of >2 were considered as positive for LF. LF immunostaining was encountered in 26.15% cases. A significant correlation was found between LF expression and a low Ki-67 labeling index (P=0.040), the absence of recurrence (P=0.010) and alive status (P=0.020). LF may assist in identifying a subset of TNBC with less aggressive biological behavior. The meaning of LF expression in TNBC remains unclear and is controversial. The present findings indicated that LF expression is correlated with a low growth fraction in these tumors. Thus, it is possible that the inhibition of the LF axis may be a valid therapeutic target for TNBC, and this should be confirmed by future studies.

show abstract

“…Together with this well documented iron-chelating activity, LF is able to modulate inflammatory processes by an anti-oxydative action [12][13][14]; more recently, it has been shown that Lf promotes the bone growth stimulating the proliferation and differentiation of osteoblasts and inhibiting their apoptosis as well as osteoclastogenesis [15][16][17]. Finally, it has been suggested that LF is able to inhibit cellular proliferation with a suppressive effect on tumours, either in vitro either in vivo [2,11,[18][19][20], although the effective molecular mechanism is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Investigation of Lactoferrin in Human Bone Primary and Metastatic Tumours

Ieni¹,

Barresi²,

Speciale³

et al. 2015

Int J Lab Med Res

View full text Add to dashboard Cite

Background: Lactoferrin (LF), an iron-binding glycoprotein, has a multifunctional role in humans, specifically in the regulation of iron homeostasis, host defense against infections and inflammations, even if some experimental studies attributed others activities to LF, such as cellular differentiation, regulation growth, protection against cancer development and metastases. Methods: We performed herein an immunohistochemical analysis of LF in a cohort of primary and metastatic neoplasms occurred in the bone by using a monoclonal specific antibody. LF reactivity was quantified using an intensity-distribution (ID) score. Results: In primary bone tumours, LF immunostaining as whole was evident in 21/82 cases (25.60%), either benign or malignant. About bone metastatic lesions, LF immunopositivity was encountered in 14/30 cases (46.6%), mainly due to prostatic, renal, uterine and colonic carcinomas, while the positivity was reduced in metastases from breast carcinomas and it was completely absent in lung cancer. Conclusion: On the light of these results, we suggest that neoplastic elements might produce LF in order to make a greater amount of iron available for their turnover. Additional analyses are needed concerning new applications of LF in clinical oncology either for its nutraceutical function either for its capability to potentiate chemotherapy.

show abstract

Immunoexpression of lactoferrin in human sporadic renal cell carcinomas

Cited by 16 publications

References 0 publications

Neutrophil gelatinase-associated lipocalin immunoexpression in renal tumors: Correlation with histotype and histological grade

Neutrophil gelatinase-associated lipocalin immunoexpression in renal tumors: Correlation with histotype and histological grade

Immunoexpression of lactoferrin in triple-negative breast cancer patients: A proposal to select a less aggressive subgroup

Immunohistochemical Investigation of Lactoferrin in Human Bone Primary and Metastatic Tumours

Contact Info

Product

Resources

About