Immunofluorescent Identification of Cells Synthesizing Human Growth Hormone in Culture

Tj, Martin; Beck, Christine; Gulì, E; Hg, Burger; Pb, Greenberg; Ra, Melick

doi:10.1055/s-0028-1094132

Cited by 3 publications

(2 citation statements)

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“…In extensive studies of PTH synthesis by human parathyroid cells (Martin et al, , 1972(Martin et al, , 1973 we have found no evidence for the existence in cells or in media of such low-molecular-weight peptides with PTH-like immunoreactivity. The peptides found in this cancer cell culture may represent synthesis of a number of incomplete PTH molecules by the cancer cells, or could result from a rapid and potent proteolytic process within the cells, leading to a rapid turnover of newly synthesized hormone and formation of fragments of the molecule.…”

Section: Discussionmentioning

confidence: 73%

“…It seems unlikely that the fragments were generated by breakdown occurring in the medium, since conditioned medium (i.e. medium removed after prolonged incubation with cells) gave rise to no fragments of 1251-labelled PTH after incubation, and furthermore, in numerous experiments with human parathyroid cell cultures, this phenomenon of smaller-molecular-weight immunoreactive fragments has not been observed Martin, Greenberg & Melick, 1972). The radioactive secretions of the cells contained a small amount of PTH and a peptide of similar molecular weight to PTH (by its elution from Sephadex G-100), but more basic in charge, resembling in this respect proPTH, the recently discovered biosynthetic precursor of PTH (Hamilton, Macgregor, Chu & Cohn, 1971;Kemper, Habener, Potts & Rich, 1972).…”

Section: Discussionmentioning

confidence: 99%

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Synthesis and Release of Parathyroid Hormone by a Renal Carcinoma in Cell Culture

1973

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1. A monolayer cell culture was established from a hypernephroma removed from a patient with the diagnosis of ectopic parathyroid hormone (PTH) production.2. Culture media contained immunoreactive peptides of a similar size to PTH and several of smaller size.3. ['4C]Leucine and "Se-labelled methionine added to the media were incorporated into those peptides in the cells and medium which behaved as PTH immunologically and physicochemically. 4. A basic peptide resembling proPTH (the biosynthetic precursor of PTH) was synthesized.5. Labelled immunoreactive fragments of PTH were found in cells after incubation with radioactive amino acids.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%