Immunofluorescent labeling of increased calpain expression and neuronal death in the spinal cord of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice

Chera, B.S.; Schaecher, Kurt E.; Rocchini, Anne; Imam, Syed Z.; Sribnick, Eric A.; Ray, Swapan K.; Ali, Syed F.; Banik, Naren L.

doi:10.1016/j.brainres.2004.01.065

Cited by 37 publications

(43 citation statements)

References 28 publications

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“…We used SIF labelings to examine overexpression of several specific proteases and then DIF stainings to determine apoptosis with involvement of the specific proteases, as we reported previously [23]. Our results indicated occurrence of apoptosis with involvement of calpain, caspase-12, and caspase-3.…”

Section: Discussionmentioning

confidence: 67%

“…Slides were mounted with VectaShield (Vector Laboratories, Burlingame, CA, USA) and viewed promptly under a non-confocal fluorescence microscope at 200· magnification (Olympus, Japan). Digital pictures were captured using the ImagePro-Plus software (Media Cybernetics, Silver Spring, MD, USA), as we described previously [23].…”

Section: Surgical Removal Of T98g Xenografts and Histopathological Evmentioning

confidence: 99%

“…Combined TUNEL and double immunofluorescent labeling for detection of apoptosis with upregulation of a cysteine protease or AIF This was performed with some modification of our previously reported method [23]. Briefly, 5 lm sections of each T98G xenograft were cut, mounted on slides, and fixed in 95% (v/v) ethanol for 10 min.…”

Section: Surgical Removal Of T98g Xenografts and Histopathological Evmentioning

confidence: 99%

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Combination of all-trans retinoic acid and taxol regressed glioblastoma T98G xenografts in nude mice

et al. 2007

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Glioblastoma is the most prevalent and highly malignant brain tumor that continues to defy current treatment strategies. This investigation used all-trans retinoic acid (ATRA) and taxol (TXL) as a combination therapy for controlling the growth of human glioblastoma T98G xenografted in athymic nude mice. Histopathological examination revealed that ATRA induced differentiation and combination of ATRA and TXL caused more apoptosis than either treatment alone. Combination therapy decreased expression of telomerase, nuclear factor kappa B (NFkappacapital VE, Cyrillic), and inhibitor-of-apoptosis proteins (IAPs) indicating suppression of survival factors while upregulated Smac/Diablo. Combination therapy also changed expression of Bax and Bcl-2 proteins leading to increased Bax:Bcl-2 ratio, mitochondrial release of cytochrome c and apoptosis-inducing factor (AIF), and activation of caspase-9. Increased activities of calpain and caspase-3 degraded 270 kD alpha-spectrin at the specific sites to generate 145 kD spectrin breakdown product (SBDP) and 120 kD SBDP, respectively. Further, increased activity of caspase-3 cleaved inhibitor-of-caspase-activated DNase (ICAD). In situ double immunofluorescent labelings showed overexpression of calpain, caspase-12, caspase-3, and AIF during apoptosis, suggesting involvement of both caspase-dependent and caspase-independent pathways for apoptosis. Our investigation revealed that treatment of glioblastoma T98G xenografts with the combination of ATRA and TXL induced differentiation and multiple molecular mechanisms for apoptosis.

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Section: Discussionmentioning

confidence: 67%

Section: Surgical Removal Of T98g Xenografts and Histopathological Evmentioning

confidence: 99%

Section: Surgical Removal Of T98g Xenografts and Histopathological Evmentioning

confidence: 99%

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Combination of all-trans retinoic acid and taxol regressed glioblastoma T98G xenografts in nude mice

et al. 2007

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“…52 Increased Capn2 expression and neuronal death in MPTP-treated mice has been previously observed. 53 Importantly, microarray and proteomic measurements seem to be complementary by providing two different sets of regulated genes. The combined regulated gene and protein list provides a much clearer picture of the biological changes occurring in the striatum following the two drug treatments.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis Revealed by Proteomic and Transcriptomic Analyses of the Striata in Two Mouse Models of Parkinson’s Disease

et al. 2008

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The molecular mechanisms underlying the changes in the nigrostriatal pathway in Parkinson's disease (PD) are not completely understood. Here, we use mass spectrometry and microarrays to study the proteomic and transcriptomic changes in the striatum of two mouse models of PD, induced by the distinct neurotoxins 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and methamphetamine (METH). Proteomic analyses resulted in the identification and relative quantification of 912 proteins with two or more unique peptides and 86 proteins with significant abundance changes following neurotoxin treatment. Similarly, microarray analyses revealed 181 genes with significant changes in mRNA, following neurotoxin treatment. The combined protein and gene list provides a clearer picture of the potential mechanisms underlying neurodegeneration observed in PD. Functional analysis of this combined list revealed a number of significant categories, including mitochondrial dysfunction, oxidative stress response, and apoptosis. These results constitute one of the largest descriptive data sets integrating protein and transcript changes for these neurotoxin models with many similar end point phenotypes but distinct mechanisms.

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“…Digital pictures were taken with ImagePro-Plus software (Media Cybernetics, Silver Spring, MD, USA), as we described previously [14].…”

Section: Hematoxylin and Eosin (H And E) Staining For Histological Exammentioning

confidence: 99%

Intracranial Stereotaxic Cannulation for Development of Orthotopic Glioblastoma Allograft in Sprague-Dawley Rats and Histoimmunopathological Characterization of the Brain Tumor

2007

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Glioblastoma is the most common brain tumor that causes significant mortality annually. Limitations of the current therapeutic regimens warrant development of new techniques and treatment strategies in orthotopic animal model for better management of this devastating brain cancer. There are only a few experimental orthotopic models of glioblastoma for pre-clinical testing. In the present investigation, we successfully implanted rat C6 cells via intracranial stereotaxic cannulation in adult Sprague-Dawley rats for development and histoimmunopathological characterization of an advanced orthotopic glioblastoma allograft model, which could be useful for investigating the course of glioblastoma development as well as for testing efficacy of new therapeutic agents. The orthotopic glioblastoma allograft was generated by intracerebral injection of rat C6 cells through a guide-cannula system and after 21 post-inoculation days the brain tumor was characterized by histoimmunopathological experiments. Histological staining and immunofluorescent labelings for TERT, VEGF, Bcl-2, survivin, XIAP, and GFAP revealed the distinct characteristics of glioblastoma in C6 allograft, which could be useful as a target for treatment with emerging new therapeutic agents. Our investigation indicated the successful development of intracranial cannulated orthotopic glioblastoma allograft in adult Sprague-Dawley rats, making it as a useful animal model of glioblastoma for pre-clinical evaluation of various therapeutic strategies for the management of glioblastoma.

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Immunofluorescent labeling of increased calpain expression and neuronal death in the spinal cord of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice

Cited by 37 publications

References 28 publications

Combination of all-trans retinoic acid and taxol regressed glioblastoma T98G xenografts in nude mice

Combination of all-trans retinoic acid and taxol regressed glioblastoma T98G xenografts in nude mice

Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis Revealed by Proteomic and Transcriptomic Analyses of the Striata in Two Mouse Models of Parkinson’s Disease

Intracranial Stereotaxic Cannulation for Development of Orthotopic Glioblastoma Allograft in Sprague-Dawley Rats and Histoimmunopathological Characterization of the Brain Tumor

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