Immunofluorescent localization of amelogenins in developing bovine teeth

Graver, Heber T.; Herold, Richard; Chung, Tai-Young; Christner, Paul J.; Pappas, Charles N.; Rosenbloom, Joel

doi:10.1016/0012-1606(78)90143-4

Cited by 41 publications

(15 citation statements)

References 35 publications

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“…Bovine ameloblasts synthesize and secrete amelogenins into enamel matrix (3). In the present study, the IDE cells adherent to developing enameloid matrices showed moderate fluorescence, indicating that they contain amelogenins.…”

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confidence: 53%

“…207, 21 MARCH 1980 final injection and the serums precipitated by 70 percent ammonium sulfate. Redissolved serum was purified by affinity column chromatography (3,7). The specificity of this antiserum to amelogenin was demonstrated previously (3) by radioimmunoassay and by immunohistochemical studies of tooth odontoblasts, dentine, pulp and stellate reticulum, tongue, salivary glands, liver, kidney, and bone in which there was a complete absence of fluorescence when the antiserum was used.…”

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confidence: 99%

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Immunohistochemical Localization of Amelogenins in Enameloid of Lower Vertebrate Teeth

Herold

Graver

Christner

1980

Science

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The indirect method of immunofluorescence was used to demonstrate the presence of amelogenins in the enameloid of teeth and dermal denticles of Chondrichthyes; in the enameloid of Teleostei and Amphibia; and in the enamel of Reptilia. Nonmammalian amelogenins are formed in the ectodermal cells of tooth organs and chemically are so similar to mammalian amelogenins that they interact with antiserum prepared from bovine enamel matrix.

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confidence: 53%

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confidence: 99%

Immunohistochemical Localization of Amelogenins in Enameloid of Lower Vertebrate Teeth

Herold

Graver

Christner

1980

Science

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“…Immunohistochemical studies on amelogenin localization were extensively performed at the light microscopic level (Graver et al, 1978;Slavkin et al, 1982;Herold et al, 1987) and also at the ultrastructural level (Nanci et al, 1985(Nanci et al, , 1987Inage et al, 1989;Uchida et al, 1989). The inner enamel epithelium, pre-ameloblasts, and ameloblasts at the secretory, transitional, and shortening stages and even at the early maturation stage showed expression of the amelogenin gene by hybridization with an antisense RNA probe, while no signals were observed with a sense RNA.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression and Localization of Amelogenin in the Rat Incisor

et al. 1996

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Gene expression and localization of amelogenin were studied in the developing rat incisor by the methods of in situ hybridization and immunohistochemistry. ISH revealed the first expression of amelogenin mRNA in the inner enamel epithelium of the cervical loop. The signals were clearly observed in pre-ameloblasts in the region bordering on predentin formation and became more intense toward the cells on the initial enamel matrix secretion. The maximal signals were found in the cytoplasm of secretory ameloblasts. From the terminal secretion zone, the signals then became gradually weaker toward the incisal edge but were still evident in the cytoplasm of shortening, transitional ameloblasts and those at the early maturation stage. No signals were found in the cells of the stratum intermedium and stellate reticulum throughout amelogenesis. Immunohistochemistry by means of an antibody against amelogenin C-telopeptide consisting of 12 amino acids revealed immunoreaction in the secretory ameloblasts reacting to the ISH. When a polyclonal antibody against amelogenin was used, immunoreaction was found in the distal ends of ruffle-ended ameloblasts (RA) in the maturation zone. Those results indicated that amelogenin is synthesized by ameloblastic cells from the inner enamel epithelium to the early maturation stage and is then resorbed by the RA.

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“…The recent progress of light and electron microscopic immunocytochemistry has enabled us to investigate the localization of biological substances with higher resolution and specificity. Using these techniques, the localization of enamel proteins in the tooth system has been studied in a number of disparate vertebrate species at the light microscopic level (GRAVER et al, 1978;HEROLD et al, 1980;SLAVKIN et al, 1982), and in mice and cows at the ultrastructural level (NANCI et al, 1985;HEROLD et al, 1987;SLAVKIN et al, 1988).…”

Section: Discussionmentioning

confidence: 99%