Immunogenetics of a macroglobulin allotype in cattle

Abstract

The paper describes a cattle serum antigen (McA2) located on a macroglobulin molecule which has its isoelectric point at pH 5 and is capable of interacting with the wheat germ lectin and concanavalin A.The specificity is inherited in a simple Mendelian manner and the gene controlling its synthesis is allelic to the one controlling the synthesis of the McAl antigen.

Rhesus Monkey <i>(Macaca mulatta)</i> Allotypes

“…Following similar studies carried out in our laboratory for McAl and McA2 [8,9], two markers of cattle macroglobulins, we are ex panding the study of rhesus monkey LDL al lotypes towards the localization of these three determinants on the LDL molecule. These studies may prove valuable in understanding how the genes controlling these markers act in biochemical terms and also in defining the LDL biosynthesis and structure.…”

Immunogenetics of the McB1 Macroglobulin Allotype in Cattle

“…McBl resulted indepedent (table II) from McAl and McA2, both of which are macroglobulin markers [6], as well as from L dlA l which is a low-density lipopro tein marker [5J. Because of the high incid ence of the B' allele (see footnote to table I), the above results obtained from associa tion analysis could not be confirmed by testcross analysis.…”

“…In previous experiments it was shown that alloimmunization with whole serum in duced the production of antibodies specific for the antigenic marker McA2 in one of the immunized animals [6], When immuniza tion was continued according to the sched ule described elsewhere [6], the same reci pient also formed antibodies for another de terminant which was termed M cBl.2 The present paper describes this new marker and its genetic relationships to other allo typic factors reported previously [5,6], The antiserum used for the identification of » The project was financed by a grant from the Consiglio Nazionale delie Ricerche (contract 78.00223.80). 2 Me denoting macroglobulin, B the locus and 1 the factor; in the text, for brevity, the prefix Me will be omitted whenever such an omission does not create ambiguity.…”

“…The paper describes a cattle serum antigen (McBl) which is controlled by a dominant gene (Me/?1), independent from those controlling the two macroglobulin mar kers McAl and McA2 as well as from that controlling the low-density lipoprotein marker L dlA l. McBl is located on a high-molecular-weight serum protein which, very likely, is an a2-rnacr°gl°biilin.Numerous serum antigens (allotypes) have been already described in cattle [2,5,6,9]. In previous experiments it was shown that alloimmunization with whole serum in duced the production of antibodies specific for the antigenic marker McA2 in one of the immunized animals [6], When immuniza tion was continued according to the sched ule described elsewhere [6], the same reci pient also formed antibodies for another de terminant which was termed M cBl.2 The present paper describes this new marker and its genetic relationships to other allo typic factors reported previously [5,6], The antiserum used for the identification of » The project was financed by a grant from the Consiglio Nazionale delie Ricerche (contract 78.00223.80).…”

Immunogenetics of the D1 Serum Antigen of Rhesus Monkey <i>(Macaca mulatta</i><i>)</i>

“…When a Dl( + ) serum was chroma tographed on Con A-Sepharose as described elsewhere [41, all antigen activity was re covered in the second peak (i.e., the one eluted upon addition of 1.0 M a-methyl-Dglucoside). When this same peak, concen trated back to its original volume (1 ml) by ultrafiltration fAmicon UM 20E mem branes), was chromatographed on WGLSepharose as already described [4], the Dt antigen bound to the resin and eluted upon addition of 0.5 M N-acetyl-D-glucosamine. These experiments, when taken together, suggest that the molecule on which the anti gen resides contains N-acetyl-D-glucosamine residues necessary for interaction with WGL and, possibly, also mannose residues.…”