Immunogenic capacity of macrophage hybridomas

Abstract

Two clones, E2-7.7 and E2-10.50, derived from two macrophage(M phi)hybridomas, E2-7 and E2-10, have been studied. The first clone, E2-7.7, is Ia+ and Fc receptor (FcR) negative and manifests a strong antigen-presenting capacity. When we pulsed its cells in vitro with keyhole limpet hemocyanin (KLH) antigen and injected them into syngeneic animals, we found that as small a dose as 10(3) cells initiated an immune response in vivo. On the other hand, antigen-pulsed cells of the E2-10.50 clone, which are Ia- and FcR+, were almost incapable of triggering immunity, even when injected at a dose of 10(5) cells. Thus, the two clones differ in their immunogenic capacity (both cellular and humoral immunity). In experiments aimed at testing the stimulation in vitro of primed lymph node (LN) cells by antigen-pulsed cells of these two hybridoma clones, we observed that E2-7.7 stimulated the unfractionated population of LN cells and the LN-derived population of T cells. The E2-10.50 cells stimulated only the unfractionated population of LN cells, but not the T cell population. Subsequent tests indicated that the E2-10.50 cells require an intermediate Ia+ accessory cell to present the antigen to the T lymphocytes. Analyzing the molecular structure of the M phi hybridomas, we discovered that major histocompatibility complex (MHC) genes of the myeloma haplotype (H-2d), and of the splenic M phi used for fusion (H-2k), which were not expressed in the parental myeloma or in the E2-10.50, were expressed in the E2-7.7. Thus, somatic cell fusion of M phi resulted in the activation of suppressed genes of the myeloma partner. It appears that these antigens participate in controlling the immunogenic properties of the E2-7.7 clone. Testing the effects of interferons on the M phi hybridomas, we observed that interferon-gamma activated, at both the mRNA and the cell surface-antigen levels, the expression of H-2Dk, H-2Kd and H-2Dd in the E2-10.50 cells, but not in the E2-7.7. Consequently, interferon-gamma augmented significantly antigen presentation by E2-10.50 but not by E2-7.7 cells. These two hybridoma clones might represent two distinct subsets of normal M phi, manifesting two different sets of functional properties.(ABSTRACT TRUNCATED AT 400 WORDS)

Involvement of major histocompatibility complex class I antigens in T cell activation

“…Similarly, these mAb suppress anti-CD3 mAb-induced Tcell activation in a monocyte-dependent manner [ 101. Furthermore, IFN-yinduced elevation in class I Ag expression on MQ hybridoma cells augments their Ag presentation capacity [29]. Therefore, class I Ag on M@ (or APC) play an important role in Ag presentation.…”