Immunogenicity and anti-Burkholderia pseudomallei activity in Balb/c mice immunized with plasmid DNA encoding flagellin

Chen, Yao Shen; Hsiao, Yu-Sheng; Lin, His Hsun; Yen, Chuan-Min; Chen, Ssu Ching; Chen, Ya Lei

doi:10.1016/j.vaccine.2005.08.069

Cited by 38 publications

(34 citation statements)

References 31 publications

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“…To improve the efficacy of the polysaccharide vaccines, it may be necessary to identify additional protein antigens that also afford protection against melioidosis. To our knowledge, only immunization with a DNA vaccine encoding the flagellin protein FliC (8,9) or sera raised against FliC (5) has been shown to afford any protection against B. pseudomallei infection in mice.…”

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confidence: 99%

Identification of a LolC Homologue inBurkholderia pseudomallei, a Novel Protective Antigen for Melioidosis

et al. 2007

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Identification of a LolC Homologue inBurkholderia pseudomallei, a Novel Protective Antigen for Melioidosis

et al. 2007

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“…It seems that the Th-1 immune response plays an important defense role in protecting against B. pseudomallei infection. However, these vaccines, which are currently under development, because they use purified somatic antigens or recombinant proteins are hampered in their ability to induce a cell-mediated immune (CMI) response, and therefore this may limit their potential (8).…”

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confidence: 99%

CpG-Modified Plasmid DNA Encoding Flagellin Improves Immunogenicity and Provides Protection againstBurkholderia pseudomalleiInfection in BALB/c Mice

et al. 2006

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“…In addition, CD4 ϩ T cells of some responders from the seropositive group could produce IFN-␥ at levels above the mean in response to the other proteins tested, including ABC (11,27). Conversely, recombinant flagellin could provide limited protection in mice, albeit not as great as that when the protein was given as a plasmid DNA vaccine or with specific adjuvants such as CpG (5,6,30). Recombinant Hsp, especially Hsp70, can induce T-cell responses due to flagellin contamination (34).…”

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confidence: 99%

“…However, antibodies to BipB, BipC, and BipD have been detected in sera of melioidosis patients (11,28). Furthermore, a DNA vaccine encoding the flagellin protein and carrying CpG motifs enhanced resistance of immunized mice to B. pseudomallei (5,6). Owing to the incomplete protection afforded by candidate antigens to date, it is necessary to search for additional proteins for vaccine development.…”

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Burkholderia pseudomalleiProteins Presented by Monocyte-Derived Dendritic Cells Stimulate Human Memory T CellsIn Vitro

et al. 2011

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Melioidosis is a severe infectious disease caused by the saprophytic facultative intracellular pathogen Burkholderia pseudomallei. The disease is endemic in Southeast Asia and Northern Australia, and no effective vaccine exists. To describe human cell-mediated immune responses to B. pseudomallei and to identify candidate antigens for vaccine development, the ability of antigen-pulsed monocyte-derived dendritic cells (moDCs) to trigger autologous T-cell responses to B. pseudomallei and its products was tested. moDCs were prepared from healthy individuals exposed or not exposed to B. pseudomallei, based on serological evidence. These were pulsed with heat-killed B. pseudomallei or purified antigens, including ABC transporters (LolC, OppA, and PotF), Bsa type III secreted proteins (BipD and BopE), tandem repeat sequence-containing proteins (Rp1 and Rp2), flagellin, and heat shock proteins (Hsp60 and Hsp70), prior to being mixed with autologous T-cell populations. After pulsing of cells with either heat-killed B. pseudomallei, LolC, or Rp2, coculturing the antigen-pulsed moDCs with T cells elicited gamma interferon production from CD4 ؉ T cells from seropositive donors at levels greater than those for seronegative donors. These antigens also induced granzyme B (cytotoxic) responses from CD8 ؉ T cells. Activation of antigen-specific CD4 ؉ T cells required direct contact with moDCs and was therefore not dependent on soluble mediators. Rp peptide epitopes recognized by T cells in healthy individuals were identified. Our study provides valuable novel data on the induction of human cell-mediated immune responses to B. pseudomallei and its protein antigens that may be exploited in the rational development of vaccines to combat melioidosis.

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Immunogenicity and anti-Burkholderia pseudomallei activity in Balb/c mice immunized with plasmid DNA encoding flagellin

Cited by 38 publications

References 31 publications

Identification of a LolC Homologue inBurkholderia pseudomallei, a Novel Protective Antigen for Melioidosis

Identification of a LolC Homologue inBurkholderia pseudomallei, a Novel Protective Antigen for Melioidosis

CpG-Modified Plasmid DNA Encoding Flagellin Improves Immunogenicity and Provides Protection againstBurkholderia pseudomalleiInfection in BALB/c Mice

Burkholderia pseudomalleiProteins Presented by Monocyte-Derived Dendritic Cells Stimulate Human Memory T CellsIn Vitro

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