Immunogenicity and protection of oral influenza vaccines formulated into microparticles

Shastri, Prathap Nagaraja; Kim, Min‐Chul; Quan, Fu‐Shi; D’Souza, Martin J.; Kang, Sang‐Moo

doi:10.1002/jps.23220

Cited by 29 publications

(27 citation statements)

References 52 publications

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“…Finally, the particles loaded with drugs obtained by spray-drying could be administered by different routes such as oral [127][128][129][130][131][132], pulmonary [12,23,82,133,134), ophthalmic [93,111,135], parenteral [22,38,136], nasal [60,120,121,137], and vaginal [138], stressing its great In vitro tests showed that their mucoadhesion was 2-fold higher than that of a hydroxyethylcellulose gel formulation [138].…”

Section: Different Routes Of Administration Of Drug-loaded Polymeric mentioning

confidence: 99%

Advantages and challenges of the spray-drying technology for the production of pure drug particles and drug-loaded polymeric carriers

Sosnik

Seremeta

2015

Advances in Colloid and Interface Science

522

252

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Section: Different Routes Of Administration Of Drug-loaded Polymeric mentioning

confidence: 99%

Advantages and challenges of the spray-drying technology for the production of pure drug particles and drug-loaded polymeric carriers

Sosnik

Seremeta

2015

Advances in Colloid and Interface Science

522

252

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“…However, in vivo animal studies have demonstrated that orally administered influenza vaccine did not produce satisfactory levels of immunogenicity compared with other routes of administration, mainly due to the destabilization of oral vaccines in the stomach [11]–[13]. Thus, to induce a similar level of protective immunogenicity, the vaccine must contain a larger quantity of antigens [8], [11], [14], [15], resulting in the decrease of economic benefits of inactivated oral influenza vaccine. As a result, despite tremendous efforts, there is still no commercially available oral influenza vaccine.…”

Section: Introductionmentioning

confidence: 99%

A Mechanistic Study on the Destabilization of Whole Inactivated Influenza Virus Vaccine in Gastric Environment

et al. 2013

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Oral immunization using whole inactivated influenza virus vaccine promises an efficient vaccination strategy. While oral vaccination was hampered by harsh gastric environment, a systematic understanding about vaccine destabilization mechanisms was not performed. Here, we investigated the separate and combined effects of temperature, retention time, pH, and osmotic stress on the stability of influenza vaccine by monitoring the time-dependent morphological change using stopped-flow light scattering. When exposed to osmotic stress, clustering of vaccine particles was enhanced in an acidic medium (pH 2.0) at ≥25°C. Fluorescence spectroscopic studies showed that hyper-osmotic stress at pH 2.0 and 37°C caused a considerable increase in conformational change of antigenic proteins compared to that in acidic iso-osmotic medium. A structural integrity of membrane was destroyed upon exposure to hyper-osmotic stress, leading to irreversible morphological change, as observed by undulation in stopped-flow light scattering intensity and transmission electron microscopy. Consistent with these analyses, hemagglutination activity decreased more significantly with an increasing magnitude of hyper-osmotic stress than in the presence of the hypo- and iso-osmotic stresses. This study shows that the magnitude and direction of the osmotic gradient has a substantial impact on the stability of orally administrated influenza vaccine.

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“…The Eudragit® family of polymers has been successfully used to deliver peptide and protein based vaccines [35–37]. Recently, these polymers have been used to encapsulate inactivated oral influenza virus vaccines [38]. The main hurdle to using polymers for encapsulation of live viral vaccines has been the toxicity of organic solvents used to manufacture the coatings for viruses.…”

Section: Discussionmentioning

confidence: 99%

Live-vaccinia virus encapsulation in pH-sensitive polymer increases safety of a reservoir-targeted Lyme disease vaccine by targeting gastrointestinal release

Kern

Zhou

Feng

et al. 2016

Vaccine

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Summary The incidence of Lyme disease has continued to rise despite attempts to control its spread. Vaccination of zoonotic reservoirs of human pathogens has been successfully used to decrease the incidence of rabies in raccoons and foxes. We have previously reported on the efficacy of a vaccinia virus vectored vaccine to reduce carriage of Borrelia burgdorferi in reservoir mice and ticks. One potential drawback to vaccinia virus vectored vaccines is the risk of accidental infection of humans. To reduce this risk, we developed a process to encapsulate vaccinia virus with a pH-sensitive polymer that inactivates the virus until it is ingested and dissolved by stomach acids. We demonstrate that the vaccine is inactive both in vitro and in vivo until it is released from the polymer. Once released from the polymer by contact with an acidic pH solution, the virus regains infectivity. Vaccination with coated vaccinia virus confers protection against B. burgdorferi infection and reduction in acquisition of the pathogen by naïve feeding ticks.

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Immunogenicity and protection of oral influenza vaccines formulated into microparticles

Cited by 29 publications

References 52 publications

Advantages and challenges of the spray-drying technology for the production of pure drug particles and drug-loaded polymeric carriers

Advantages and challenges of the spray-drying technology for the production of pure drug particles and drug-loaded polymeric carriers

A Mechanistic Study on the Destabilization of Whole Inactivated Influenza Virus Vaccine in Gastric Environment

Live-vaccinia virus encapsulation in pH-sensitive polymer increases safety of a reservoir-targeted Lyme disease vaccine by targeting gastrointestinal release

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