Immunogenicity and protective efficacy of Recombinase A from Wolbachia endosymbiont of filarial nematode Brugia malayi (wBmRecA)

Gangwar, Mamta; Jha, Ruchi; Goyal, Manish; Srivastava, Mrigank

doi:10.1016/j.vaccine.2018.12.015

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…It remains a challenging prospect to design vaccines for LF because the population in the endemic region is continuously exposed to different stages of the parasite, which vary significantly in their antigenic components. − Nevertheless, recent advances in the understanding of the disease etiology coupled with a few promising leads have generated hope that vaccine development for filarial infection is an achievable goal that is very desirable. − …”

Section: Discussionmentioning

confidence: 99%

Immunization with Brugia malayi Calreticulin Protein Generates Robust Antiparasitic Immunity and Offers Protection during Experimental Lymphatic Filariasis

Yadav¹,

Sharma²,

Sharma

et al. 2021

ACS Infect. Dis.

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Lymphatic filariasis causes permanent and long-term disability worldwide. Lack of potent adulticidal drugs, the emergence of drug resistance, and the nonavailability of effective vaccines are the major drawbacks toward LF elimination. However, immunomodulatory proteins present in the parasite secretome are capable of providing good protection against LF and thus offer hope in designing new vaccines against LF. Here, we evaluated the immunogenicity and protective efficacy of B. malayi calreticulin protein (BmCRT) using in vitro and in vivo approaches. Stimulation with recombinant BmCRT (rBmCRT) significantly upregulated Th1 cytokine production in mouse splenocytes, mesenteric lymph nodes (mLNs), and splenic and peritoneal macrophages (PMΦs). Heightened NO release, ROS generation, increased lymphocyte proliferation, and increased antigen uptake were also observed after rBmCRT exposure. Mice immunized with rBmCRT responded with increased Th1 and Th2 cytokine secretion and exhibited highly elevated titers of anti-BmCRT specific IgG at day 14 and day 28 postimmunization while splenocytes and mLNs from immunized mice showed a robust recall response on restimulation with rBmCRT. Infective larvae (L3) challenge and protection studies undertaken in Mastomys coucha, a permissive model for LF, showed that rBmCRT-immunized animals mounted a robust humoral immune response as evident by elevated levels of total IgG, IgG1, IgG2a, IgG2b, and IgG3 in their serum even 150 days after L3 challenge, which led to significantly reduced microfilariae and worm burden in infected animals. BmCRT is highly immunogenic and generates robust antiparasitic immunity in immunized animals and should therefore be explored further as a putative vaccine candidate against LF.

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Section: Discussionmentioning

confidence: 99%

Immunization with Brugia malayi Calreticulin Protein Generates Robust Antiparasitic Immunity and Offers Protection during Experimental Lymphatic Filariasis

Yadav¹,

Sharma²,

Sharma

et al. 2021

ACS Infect. Dis.

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“…The B. malayi trehalose-6-phosphate phosphatase antigen induced high levels of CD8 + T cells as part of the host antigen-specific cellular immune responses ( 35 ). Immunization of mice with B. malayi Wolbachia recombinase A resulted in a significant expansion of CD8 + T cells in the host spleen ( 36 ). Studies in TCR transgenic mice have also shown that the O. volvulus antigens O v ALT-2 and O v NLT-1 exert suppressive effects on CD8 + T cell proliferation and cytokine release ( 37 ).…”

Section: Cd8 + T Cells In Anti-filarial Immunitymentioning

confidence: 99%

Highlighting the Relevance of CD8+ T Cells in Filarial Infections

et al. 2021

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The T cell immune responses in filarial infections are primarily mediated by CD4+ T cells and type 2-associated cytokines. Emerging evidence indicates that CD8+ T cell responses are important for anti-filarial immunity, however, could be suppressed in co-infections. This review summarizes what we know so far about the activities of CD8+ T cell responses in filarial infections, co-infections, and the associations with the development of filarial pathologies.

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Biochemical characterization of Recombinase A from Wolbachia endosymbiont of filarial nematode Brugia malayi (wBmRecA)

Gangwar

Jha

Goyal

et al. 2021

International Journal for Parasitology

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Immunogenicity and protective efficacy of Recombinase A from Wolbachia endosymbiont of filarial nematode Brugia malayi (wBmRecA)

Cited by 5 publications

References 41 publications

Immunization with Brugia malayi Calreticulin Protein Generates Robust Antiparasitic Immunity and Offers Protection during Experimental Lymphatic Filariasis

Immunization with Brugia malayi Calreticulin Protein Generates Robust Antiparasitic Immunity and Offers Protection during Experimental Lymphatic Filariasis

Highlighting the Relevance of CD8+ T Cells in Filarial Infections

Biochemical characterization of Recombinase A from Wolbachia endosymbiont of filarial nematode Brugia malayi (wBmRecA)

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