Immunogenicity of Lyophilized MVA Vaccine for HIV-1 in Mice Model

Zhang, Y; Jiang, Chunlai; Yu, Xianghui; Lou, Chenwei; Zhao, Dandan; Wu, Yongge; Liu, C; Kong, Wei

doi:10.1016/s1005-9040(07)60070-1

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…After the construction of the vector MVA/FIV-B, the shelf life was evaluated upon lyophilization followed by a period of three months in refrigerator temperature without stabilizers. The results show that MVA/FIV-B did not decrease the viral titers over time, corroborating previous works that have shown the stability of MVA upon storage under different conditions and for periods longer than one year [ 27 , 28 ]. This is a positive quality that favors the distribution of this vaccine, giving a commercial advantage to the product.…”

Section: Discussionsupporting

confidence: 90%

Developing a Feline Immunodeficiency Virus Subtype B Vaccine Prototype Using a Recombinant MVA Vector

et al. 2022

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The feline immunodeficiency virus (FIV) is a retrovirus with global impact and distribution, affecting both domestic and wild cats. This virus can cause severe and progressive immunosuppression culminating in the death of felids. Since the discovery of FIV, only one vaccine has been commercially available. This vaccine has proven efficiency against FIV subtypes A and D, whereas subtype B (FIV-B), found in multiple continents, is not currently preventable by vaccination. We, therefore, developed and evaluated a vaccine prototype against FIV-B using the recombinant viral vector modified vaccinia virus Ankara (MVA) expressing the variable region V1–V3 of the FIV-B envelope protein. We conducted preclinical tests in immunized mice (C57BL/6) using a prime-boost protocol with a 21 day interval and evaluated cellular and humoral responses as well the vaccine viability after lyophilization and storage. The animals immunized with the recombinant MVA/FIV virus developed specific splenocyte proliferation when stimulated with designed peptides. We also detected cellular and humoral immunity activation with IFN-y and antibody production. The data obtained in this study support further development of this immunogen and testing in cats.

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Section: Discussionsupporting

confidence: 90%

Developing a Feline Immunodeficiency Virus Subtype B Vaccine Prototype Using a Recombinant MVA Vector

et al. 2022

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“…(7) MVA vaccines can induce CD4 and CD8 T cell responses important for protection against some viral infections (Amara et al, 2002). (8) A lyophilized MVA vaccine can be stored at 37 C for 2 weeks and at 4 C for longer than a year (Zhang et al, 2007). (9) MVA-based vaccines have been shown to protect against SARS-CoV, MERS-CoV, Zika virus, and Ebola virus in animal models (Bisht et al, 2004;Brault et al, 2017;Domi et al, 2018;Haagmans et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs

et al. 2021

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A combination of vaccination approaches will likely be necessary to fully control the SARS-CoV-2 pandemic. Here, we show that modified vaccinia Ankara (MVA) vectors expressing membrane anchored pre-fusion stabilized spike (MVA/S), but not secreted S1, induced strong neutralizing antibody responses against SARS-CoV-2 in mice. In macaques, the MVA/S vaccination induced strong neutralizing antibodies and CD8 + T cell responses, and showed protection from SARS-CoV-2 infection and virus replication in the lung as early as day 2 following intranasal or intratracheal challenge. Single-cell RNA sequencing analysis of lung cells at day 4 post-infection revealed that MVA/S vaccination also protected macaques from infection-induced inflammation and B cell abnormalities, and lowered induction of interferon stimulated genes. These results demonstrate that MVA/S vaccination induces both neutralizing antibodies and CD8 + T cells in the blood and lung and serves as a potential vaccine candidate against SARS-CoV-2.

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“…Before 2000, the "iron rice bowl" system covered all university faculty members (Wang et al, 2019;Xia et al, 2020b). This has changed in the years since September 1999, when the government decided to replace lifetime employment with an all-member contract system in higher education institutions (Sun, 2010;Zhang et al, 2016). Although this reform has failed to transform faculty members on the state payroll into contract employees (Xia et al, 2020b), it has enabled universities to introduce flexibility and non-socialist logics into the workplace.…”

Section: Reducing Incompatibility Of Multiple Logics By Constructing ...mentioning

confidence: 99%

“…It can help organizations obtain staff with the proper competencies and values that fit their needs. Under the "iron rice bowl" system, selective staffing was impossible since recruitment and selection were centrally controlled by the government and universities had little say in staffing (Xia et al, 2020b;Zhang et al, 2016). Since the mid-1990s, recruitment and selection in universities have transformed from central allocation to autonomous selection, and from close organizational retention to open recruitment and strict selection (interviews with R-M09; TR-M05; TR-M11).…”

Section: Conducting Rigorous and Selective Staffingmentioning

confidence: 99%

Reconciling multiple institutional logics for ambidexterity: human resource management reforms in Chinese public universities

et al. 2023

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Over the past several decades, Chinese universities have introduced various Western-style human resource management (HRM) practices to improve organizational performance. Such HRM innovations have resulted in new employment relations and paradoxical HR practices, which cannot be explained by the unitary institutional logic assumed by conventional HRM theories. Based on in-depth interviews of academic and administrative members, this study examines how Chinese universities struggle to reconcile competing institutional logics through HR innovations for ambidexterity. Our research reveals a unique transformation trajectory of personnel management in Chinese public universities. The findings indicate that human resource management in Chinese universities has been influenced by multiple logics of socialism, market, and corporation, heading along the neoliberal and managerial route while being shaped by strong state regulations. Chinese universities still have a long way to go to reconcile multiple institutional logics and achieve ambidexterity.

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Immunogenicity of Lyophilized MVA Vaccine for HIV-1 in Mice Model

Cited by 3 publications

References 7 publications

Developing a Feline Immunodeficiency Virus Subtype B Vaccine Prototype Using a Recombinant MVA Vector

Developing a Feline Immunodeficiency Virus Subtype B Vaccine Prototype Using a Recombinant MVA Vector

A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs

Reconciling multiple institutional logics for ambidexterity: human resource management reforms in Chinese public universities

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