Immunogenicity of mitochondrial DNA modified by hydroxyl radical

Alam, Khursheed; Moinuddin, Mohammed; Jabeen, S.

doi:10.1016/j.cellimm.2007.06.007

Cited by 49 publications

(38 citation statements)

References 31 publications

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“…In patients with SLE, ROS that are primarily derived from the mitochondria of lymphocytes, vascular endothelial cells, and erythrocytes have been involved in the apoptosis of T cell and the pathogenesis of SLE [29,[34][35][36]. Similarly, the rate of eryptosis was positively correlated with the ROS abundance as shown in this study.…”

Section: Discussionsupporting

confidence: 75%

Eryptosis as an Underlying Mechanism in Systemic Lupus Erythematosus-Related Anemia

Jiang

Bian²,

Ma³

et al. 2016

Cell Physiol Biochem

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Background: The progression of systemic lupus erythematosus (SLE) leads to anemia in patients, adversely affecting prognosis. The diverse causes of anemia may include excessive eryptosis or premature suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine (PS) exposure on the cell surface. The present study explored if SLE enhances eryptosis and the underlying mechanisms. Materials and Methods: Eryptosis was assessed using flow cytometry in healthy volunteers (n = 20) and anemic patients hospitalized for SLE (n = 22), for parameters including PS exposure, cell volume, cytosolic calcium ion (Ca²⁺) levels and reactive oxygen species (ROS) and ceramide abundance. These indicators were measured in erythrocytes of experimental subjects and erythrocytes treated with plasma from healthy volunteers or SLE patients. Results: The hemoglobin and hematocrit levels were significantly lower in anemic SLE patients than in healthy volunteers (***p<0.001, p<0.001, respectively). The percentage of PS-exposing erythrocytes was significantly higher in SLE patients than in healthy volunteers (p<0.001), accompanied by an increase in cytosolic Ca²⁺ levels, oxidative stress. The measurements of PS and Ca²⁺ levels were significantly higher in the erythrocytes of healthy volunteers following incubation in plasma of SLE patients than in plasma of healthy volunteers for 24h (***p<0.001, *p<0.05 respectively). Conclusion: Eryptosis is enhanced in SLE and may contribute to anemia. The probable underlying mechanisms may be an excessive formation of ROS in erythrocytes. Also, some plasma components may trigger eryptosis by increasing the cytosolic Ca²⁺ concentration.

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Section: Discussionsupporting

confidence: 75%

Eryptosis as an Underlying Mechanism in Systemic Lupus Erythematosus-Related Anemia

Jiang

Bian²,

Ma³

et al. 2016

Cell Physiol Biochem

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“…Binding specificity of antibodies was ascertained by inhibition ELISA [30]. Varying amounts of inhibitors (0-20 lg/ml) were mixed with constant amount of antiserum or IgG.…”

Section: Inhibition Elisamentioning

confidence: 99%

A clinical correlation of anti-DNA-AGE autoantibodies in type 2 diabetes mellitus with disease duration

Ashraf

Arfat

Arif³

et al. 2015

Cellular Immunology

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“…Immunoglobulin G isolation using protein A-agarose affinity chromatography IgG were isolated from the sera of patients as well as healthy individuals using protein A-agarose column as previously described method [26].…”

Section: Collection Of Samplesmentioning

confidence: 99%

Neo-epitopes on crotonaldehyde modified DNA preferably recognize circulating autoantibodies in cancer patients

et al. 2015

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DNA damage is one of the leading causes of various pathological conditions including carcinogenesis. Crotonaldehyde is a 4-carbon unsaturated bifunctional aldehyde which is found ubiquitously and produced both exogenously and endogenously. It reacts with deoxyguanosine and form adducts with DNA. These adducts were detected and found involved in tumor formation in rats treated with crotonaldehyde. In the present study, structural changes in DNA by crotonaldehyde were evaluated by Fourier transform infrared (FTIR) spectroscopy, differential scanning colorimetry (DSC), dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), and atomic force microscopy (AFM). Enhanced binding was observed in cancer autoantibodies with the DNA modified by crotonaldehyde than the native counterpart. Immunological studies revealed enhanced binding of cancer autoantibodies with crotonaldehyde modified DNA, compared to the native form. Furthermore, lymphocyte DNA isolated from cancer patients demonstrated considerable recognition of anti-Cro-DNA IgG as compared to the DNA from healthy individuals. Therefore, we suggest that crotonaldehyde modified DNA presents unique epitopes, that may trigger autoantibody induction in cancer patients.

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Immunogenicity of mitochondrial DNA modified by hydroxyl radical

Cited by 49 publications

References 31 publications

Eryptosis as an Underlying Mechanism in Systemic Lupus Erythematosus-Related Anemia

Eryptosis as an Underlying Mechanism in Systemic Lupus Erythematosus-Related Anemia

A clinical correlation of anti-DNA-AGE autoantibodies in type 2 diabetes mellitus with disease duration

Neo-epitopes on crotonaldehyde modified DNA preferably recognize circulating autoantibodies in cancer patients

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