Immunogenicity of parenterally delivered plant-derived small and medium surface antigens of hepatitis B virus

Fedorowicz-Strońska, Olga; Kapusta, Józef; Czyż, Marcin; Kaczmarek, M.; Pniewski, Tomasz

doi:10.1007/s00299-016-1944-8

Cited by 8 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As cost‐effective, carbon‐neutral factories, plants are attractive platforms for the production of biopharmaceuticals, vaccines, and other high‐value bioproducts using advanced biotechnological tools. During the past decades, considerable efforts have been made towards using plants as production hosts for vaccines against diseases such as Alzheimer’s disease (Ishiura and Yoshida, 2019 ), cancer (Wong‐Arce et al., 2017 ), dengue fever (van Eerde et al., 2019 ), hepatitis B (Dobrica et al., 2017 ; Fedorowicz‐Stronska et al., 2016 ; Pniewski et al., 2018 ) or poliomyelitis (Chan et al., 2016 ). However, the immunogenicity of plant‐made antigens varies considerably and can be influenced substantially by the plant‐specific protein glycosylation.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus‐neutralizing activity in vaccinated mice

Dobrica

Eerde

Țucureanu

et al. 2021

Plant Biotechnology Journal

View full text Add to dashboard Cite

Chronic infection with hepatitis C virus (HCV) remains a leading cause of liver-related pathologies and a global health problem, currently affecting more than 71 million people worldwide. The development of a prophylactic vaccine is much needed to complement the effective antiviral treatment available and achieve HCV eradication. Current strategies focus on increasing the immunogenicity of the HCV envelope glycoprotein E2, the major target of virus-neutralizing antibodies, by testing various expression systems or manipulating the protein conformation and the N-glycosylation pattern. Here we report the first evidence of successful production of the fulllength HCV E2 glycoprotein in Nicotiana benthamiana, by using the Agrobacterium-mediated transient expression technology. Molecular and functional analysis showed that the viral protein was correctly processed in plant cells and achieved the native folding required for binding to CD81, one of the HCV receptors. N-glycan analysis of HCV-E2 produced in N. benthamiana and mammalian cells indicated host-specific trimming of mannose residues and possibly, protein trafficking. Notably, the plant-derived viral antigen triggered a significant immune response in vaccinated mice, characterized by the presence of antibodies with HCV-neutralizing activity. Together, our study demonstrates that N. benthamiana is a viable alternative to costly mammalian cell cultures for the expression of complex viral antigens and supports the use of plants as cost-effective production platforms for the development of HCV vaccines.

show abstract

Section: Discussionmentioning

confidence: 99%

Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus‐neutralizing activity in vaccinated mice

Dobrica

Eerde

Țucureanu

et al. 2021

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

Expression of an immunogenic LTB-based chimeric protein targeting Zaire ebolavirus epitopes from GP1 in plant cells

et al. 2016

View full text Add to dashboard Cite

An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to fight this disease are needed. Since vaccination is an intervention that could achieve the control of this epidemic disease, exploring the production of new low-cost vaccines is a key path to consider; especially in developing countries. In this context, plants are attractive organisms for the synthesis and delivery of subunit vaccines. This study aimed at producing a chimeric protein named LTB-EBOV, based on the B subunit of the Escherichia coli heat-labile enterotoxin as an immunogenic carrier and two epitopes from the Zaire ebolavirus GP1 protein recognized by neutralizing antibodies. The LTB-EBOV protein was expressed in plant tissues at levels up to 14.7 µg/g fresh leaf tissue and proven to be immunogenic in BALB/c mice when administered by either subcutaneous or oral routes. Importantly, IgA and IgG responses were induced following the oral immunization. The potential use of the plant-made LTB-EBOV protein against EBOV is discussed.

show abstract

Corn-based vaccines: current status and prospects

Rosales‐Mendoza

Sández-Robledo

Bañuelos-Hernández

et al. 2017

Planta

View full text Add to dashboard Cite

Corn is an attractive host for vaccine production and oral delivery. The present review provides the current outlook and perspectives for this field. Among seed-crops, corn represents a key source of biomass for food, fuel production, and other applications. Since the beginning of the development of plant-based vaccines, corn was explored for the production and delivery of vaccines. About a dozen of pathogens have been studied under this technology with distinct degrees of development. A vaccine prototype against enterotoxigenic Escherichia coli was evaluated in a phase I clinical trial and several candidates targeting bacterial and viral diseases are under preclinical evaluation. The present review provides an updated outlook on this topic highlighting the employed expression strategies; perspectives for the field are also provided.

show abstract

Immunogenicity of parenterally delivered plant-derived small and medium surface antigens of hepatitis B virus

Cited by 8 publications

References 5 publications

Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus‐neutralizing activity in vaccinated mice

Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus‐neutralizing activity in vaccinated mice

Expression of an immunogenic LTB-based chimeric protein targeting Zaire ebolavirus epitopes from GP1 in plant cells

Corn-based vaccines: current status and prospects

Contact Info

Product

Resources

About