Immunogenicity of peptide‐based vaccine composed of epitopes from <i>Echinococcus granulosus</i> <scp>rEg</scp>.<scp>P29</scp>

Lv, Yongxue; Chang, Liangliang; Yang, Jihui; Wen, Jia; Zhao, Yinqi; Zhu, Mingzhao; Wu, Changyou; Zhao, Wei

doi:10.1096/fj.202201636r

Cited by 4 publications

(7 citation statements)

References 33 publications

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“…Therefore, resection of a pulmonary nodule through a laparotomy and transdiaphragmatic approach has also been described in the literature [ 46 ]. Interestingly, accumulating recent research is focused on developing vaccines against E. granulosus and E. multilocularis infections [ 47 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

et al. 2023

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Human echinococcosis is a zoonotic infection caused by the larvae of the tapeworm species Echinococcus. The liver is the most common location for a primary echinococcosis. However, the parasite may bypass or spread from the liver to the lungs, causing primary or secondary pulmonary echinococcosis, respectively. Pulmonary echinococcosis is a clinically challenging condition in which anthelminthic regiments are important, but surgery has the central role in removing the cysts and preventing recurrences. Surgical treatment may involve cystotomy, enucleation, capitonnage, or atypical resections, which occasionally are in combination with hepatic procedures. The utilization of modern devices is greatly underdescribed in surgery for thoracic infections, even though these facilitate much of the work. Therefore, this article aims to describe pulmonary echinococcosis and the role of modern surgical devices in the treatment process. Furthermore, we report surgical treatment of three different cases of pulmonary echinococcosis. Surgeries of uncomplicated and ruptured hepatic or pulmonary cysts are described. Simple small pulmonary echinococcal lesions can be excised by endostaplers both for diagnostic and curative reasons. Larger cysts can be removed by energy devices unless large bronchial air leaks occur. Complicated cysts require treatment by more extensive techniques. Inexperienced surgeons should not abstain but should carefully decide preoperatively how to proceed.

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Section: Discussionmentioning

confidence: 99%

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

et al. 2023

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“…Then, proteins were filtered using a 0.22‐μm sterilization filter to obtain rEg.P29 that met the experimental criteria. Epitope peptide screening was carried out by the overlapping peptide method, and the final screening yielded the dominant T‐cell epitope (rEg.P29 86‐100 [ALEQVASQSEKAAPQ]) and B‐cell epitope (rEg.P29 166‐185 [LKNAKTAEQKAKWEAEVRKD]) of rEg.P29 16 . The synthetic peptides were submitted to Sangon Biotech (Shanghai, China) for synthesis, and three epitope peptide vaccines (rEg.P29 T , rEg.P29 B , and rEg.P29 T + B ) were obtained by coupling carrier proteins (keyhole limpet hemocyanin, KLH) to the screened T‐ and B‐cell epitope peptides.…”

Section: Methodsmentioning

confidence: 99%

“…The synthetic peptides were submitted to Sangon Biotech (Shanghai, China) for synthesis, and three epitope peptide vaccines (rEg.P29 T , rEg.P29 B , and rEg.P29 T + B ) were obtained by coupling carrier proteins (keyhole limpet hemocyanin, KLH) to the screened T‐ and B‐cell epitope peptides. The rEg.P29 T + B was constructed by coupling the T‐cell epitope peptide and the B‐cell epitope peptide to the KLH vector at the N‐terminal end of the B‐cell epitope peptide after linking the two peptides using a universal linker, GSGSGS, to obtain a combined T‐cell and B‐cell epitope peptide 16 . The adjuvant CpG ODN 1826 (TCCAT GAC GTT CCT GACGTT) was synthesized by Sangon.…”

Section: Methodsmentioning

confidence: 99%

“…Epitope peptide screening was carried out by the overlapping peptide method, and the final screening yielded the dominant T-cell epitope (rEg.P29 86-100 [ALEQ-VASQSEKAAPQ]) and B-cell epitope (rEg.P29 166-185 [LKNAKTAEQKAKWEAEVRKD]) of rEg.P29. 16 The synthetic peptides were submitted to Sangon Biotech (Shanghai, China) for synthesis, and three epitope peptide vaccines (rEg.P29 T , rEg.P29 B , and rEg.P29 T + B ) were obtained by coupling carrier proteins (keyhole limpet hemocyanin, KLH) to the screened T-and B-cell epitope peptides. The rEg.P29 T + B was constructed by coupling the T-cell epitope peptide and the B-cell epitope peptide to the KLH vector at the N-terminal end of the B-cell epitope peptide after linking the two peptides using a universal linker, GSGSGS, to obtain a combined T-cell and B-cell epitope peptide.…”

Section: Protein Purification and Vaccine Preparationmentioning

confidence: 99%

“…The rEg.P29 T + B was constructed by coupling the T-cell epitope peptide and the B-cell epitope peptide to the KLH vector at the N-terminal end of the B-cell epitope peptide after linking the two peptides using a universal linker, GSGSGS, to obtain a combined T-cell and B-cell epitope peptide. 16 The adjuvant CpG ODN 1826 (TCCAT GAC GTT CCT GACGTT) was synthesized by Sangon.…”

Section: Protein Purification and Vaccine Preparationmentioning

confidence: 99%

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Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines

Zhang,

Lv,

Zhao

et al. 2023

Immunity Inflam & Disease

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ObjectiveCystic echinococcosis (CE), a zoonotic parasitic disease caused by Echinococcus granulosus, remains a public health and socioeconomic issue worldwide, making its prevention and treatment of vital importance. The aim of this study was to investigate changes in the intestinal microbiota of mice immunized with three peptide vaccines based on the recombinant antigen of E. granulosus, P29 (rEg.P29), with the hope of providing more valuable information for the development of vaccines against CE.MethodsThree peptide vaccines, rEg.P29T, rEg.P29B, and rEg.P29T + B, were prepared based on rEg.P29, and a subcutaneous immunization model was established. The intestinal floras of mice in the different immunization groups were analyzed by 16 S rRNA gene sequencing.ResultsThe intestinal microbiota analysis at both immunization time points revealed that Firmicutes, Bacteroidota, and Verrucomicrobiota were the predominant flora at the phylum level, while at the genus level, Akkermansia, unclassified_Muribaculaceae, Lachnospiraceae_NK4A136_group, and uncultured_rumen bacterium were the dominant genera. Some probiotics in the intestines of mice were significantly increased after immunization with the peptide vaccines, such as Lactobacillus_taiwanensis, Lactobacillus_reuteri, Lachnospiraceae_NK4A136_group, Bacteroides_acidifaciens, and so forth. Meanwhile, some harmful or conditionally pathogenic bacteria were decreased, such as Turicibacter sanguinis, Desulfovibrio_fairfieldensis, Clostridium_sp, and so forth, most of which are associated with inflammatory or infectious diseases. Kyoto Encyclopaedia of Genes and Genomes enrichment analysis revealed that the differential flora were enriched in multiple metabolic pathways, primarily biological systems, human diseases, metabolism, cellular processes, and environmental information processing.ConclusionIn this study, we comprehensively analyzed and compared changes in the intestinal microbiota of mice immunized with three peptide vaccines as well as their related metabolic pathways, providing a theoretical background for the development of novel vaccines against E. granulosus.

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Design of a novel EmTSP-3 and EmTIP based multi-epitope vaccine against Echinococcus multilocularis infection

Fan,

He,

et al. 2024

Front. Immunol.

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BackgroundCurrent treatments and prevention strategies for echinococcosis are inadequate. Recent advancements in molecular vaccine development show promise against Echinococcus granulosus; however, Echinococcus multilocularis remains a challenge. A Multi-epitope Vaccine could potentially induce specific B and T lymphocyte responses, thereby offering protection against Echinococcus multilocularis infection.MethodsThis study aimed to develop a MEV against alveolar echinococcosis. Key epitopes from the Echinococcus multilocularis proteins EmTSP3 and EmTIP were identified using immunoinformatics analyses. These analyses were conducted to assess the MEV feasibility, structural characteristics, molecular docking, molecular dynamics simulations, and immune simulations. The immunogenicity and antigenicity of the vaccine were evaluated through in vitro and in vivo experiments, employing ELISA, Western blotting, FCM, challenge infection experiments, and ELISPOT.ResultsThe effective antigenicity and immunogenicity of MEV were demonstrated through immunoinformatics, as well as in vitro and in vivo experiments. In vitro experiments revealed that MEV increased the secretion of IFN-γ and IL-4 in PBMC and successfully bound to specific antibodies in patient serum. Furthermore, mice immunized with MEV developed a robust immune response, characterized by elevated levels of CD4+ and CD8+ T-cells, increased secretion of IFN-γ and IL-4 by specific Th1 and Th2 cells, and heightened serum antibody levels. Importantly, MEV reduced the weight of cysts by conferring resistance against echinococcosis. These findings suggest that MEV is a promising candidate for the prevention of Echinococcus multilocularis infection.ConclusionA total of 7 CTL, 7 HTL, 5 linear B-cell, and 2 conformational B-cell epitopes were identified. The vaccine has demonstrated effective antigenicity and immunogenicity against AE through molecular docking, immune simulation, molecular dynamics studies, and both in vitro and in vivo experiments. It provides effective protection against Echinococcus multilocularis infection, thereby laying a foundation for further development.

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Immunogenicity of peptide‐based vaccine composed of epitopes from Echinococcus granulosus rEg.P29

Cited by 4 publications

References 33 publications

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices

Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines

Design of a novel EmTSP-3 and EmTIP based multi-epitope vaccine against Echinococcus multilocularis infection

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