Immunogenicity of tumour cells modified with various chemicals

Staab, H. J.; Anderer, F. A.

doi:10.1038/bjc.1977.60

Cited by 7 publications

(2 citation statements)

References 8 publications

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“…Multiple injections of inactivated cells are required to elicit a protective response with weakly antigenic tumors [15,16,30]. Systematic studies therefore were carried out to determine the optimum dose-regimens for immunizing mice to X-irradiated cells of the IP line of WEHI tumor cells.…”

Section: Lmmunogenicity Of X-irradiated Cells Given In Various Dose Rmentioning

confidence: 99%

Relative effectiveness of methods for immunizing BALB/cwm mice to transplantable myelomonocytic leukemia

Urnovitz

Murphy

1979

Leukemia Research

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~An experimental model of transplantable myelomonocytic leukemia (WEHI-3B) was developed in syngeneic BALB/cwm mice to characterize immune mechanisms in this disease. The relative effectiveness of various methods for immunizing mice to transplantable myelomonocytic cells was analyzed. When mice were immunized i.p. once a week for 3 successive weeks with cell preparations (10 T) inactivated by formalin they died at a faster rate than nonimmunized controls. When complete Freund's adjuvant was used to enhance the protective response immunized mice also died at a faster rate than the controls. If mice were immunized with various ratios of Xirradiated and viable tumor cells survival time was not increased. The most consistent result was accelerated death in the various experimental groups. When mice were immunized once weekly with X-ray inactivated cells (5000 R) for 3, 5 or8 successive weeks, the survival frequency was approximately the same (70-80%) in all three test groups. However, animals immunized 5 times withstood a higher challenge dose of tumor cells than those immunized 3 times. The spacings between immunizing doses and challenge appeared to significantly affect the efficacy of immunization. The age of mice had a significant effect on the protective response. When mice 6 weeks of age or younger were immunized the survival frequency was < 30~o, compared to 70-80~ in mice 7 weeks to 8 months of age. Considered together the results of these studies show that tumor cells of the WEHI-3B line of transplantable myelomonocytie leukemia are similar to syngeneic P1798 transplantable lymphoma and the various BALB plasmacytomas in that they are only weakly immunogenic in the BALB/c strain. The results also suggest that the immunologic response of BALB/c mice to weakly antigenic tumors is under strong negative regulation.

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Section: Lmmunogenicity Of X-irradiated Cells Given In Various Dose Rmentioning

confidence: 99%

Relative effectiveness of methods for immunizing BALB/cwm mice to transplantable myelomonocytic leukemia

Urnovitz

Murphy

1979

Leukemia Research

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“…THE ATTENUATION of tumour cells by chemical treatment before using them as immunogens has been considered attractive from several viewpoints (Prager & Baechtel, 1973;Sanderson & Frost, 1974;Staab & Anderer, 1977), particularly since previous studies have indicated that immunization with chemically modified antigens, such as flagellin, results in enhanced cell-mediated immunity and depressed antibody responses (e.g. Parish & Liew, 1972).…”

mentioning

confidence: 99%

Modification of the immunogenicity and antigenicity of rat hepatoma cells. I. Cell-surface stabilization with glutaraldehyde

Price¹,

Dennick²,

Robins³

et al. 1979

Br J Cancer

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The humoral antibody response of rats immunized with chemically modified syngeneic brain cells and glioma cells

Stavrou

Hultén

Anzil

et al. 1980

Intl Journal of Cancer

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Six-month-old Fischer rats (F344) were given the carcinogen methylnitrosourea in their drinking water. Of the induced brain tumors, four were established in culture and propagated as 78FR-G-219 (pleomorphic glioma), 78FR-G-299 (astrocytoma), 78FR-G-284 and 78FR-G-344 (mixed glioma) permanent lines. All cell lines produced S-100 protein and grew as tumors when inoculated s.c. or i.c. in syngeneic hosts. A comparative study of the antigenicity of these lines at different passage levels was carried out using native and chemically modified cells. Syngeneic rats were immunized with cells conjugated with dimethylsulfate and trinitrobenzene sulfonic acid. The immune response was characterized and quantified by an indirect immunofluorescence method and by a complement-dependent microcytotoxicity test. Chemical modification of the tumor cells enhanced antigenicity of the treated cells. The best results were attained with trinitrobenzene sulfonic-acid-treated cells and constituted a two-fold increase in the cytotoxicity index. Cytotoxicity values varied in the different cell lines. Antisera raised with trinitrobenzene sulfonic-acid-modified cells of all lines cross-reacted with cells of all lines. Cytotoxicity values were insignificantly reduced by absorbing the antisera with a variety of syngeneic tissues. Antisera raised against native syngeneic brain cells showed virtually no cytotoxicity for glioma cells. Antisera raised against syngeneic brain cells treated with trinitrobenzene sulfonic acid, however, were slightly cytotoxic for normal brain cells and glioma cells as well. The results of the present studies show that antigenicity of glioma cells can be definitely raised with trinitrobenzene sulfonic acid treatment. Furthermore, it would seem that haptenization of glioma-associated antigens may be a promising approach ot the study of glioma-host interactions.

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Immunogenicity of tumour cells modified with various chemicals

Abstract: Summary.-Mouse tumour cells were treated with various chemical modifiers. The number of modifying groups per cell was determined with labelled reagents.

Cited by 7 publications

References 8 publications

Relative effectiveness of methods for immunizing BALB/cwm mice to transplantable myelomonocytic leukemia

Relative effectiveness of methods for immunizing BALB/cwm mice to transplantable myelomonocytic leukemia

Modification of the immunogenicity and antigenicity of rat hepatoma cells. I. Cell-surface stabilization with glutaraldehyde

The humoral antibody response of rats immunized with chemically modified syngeneic brain cells and glioma cells

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