Immunogenicity of unprocessed and photooxidized bovine and human osteochondral grafts in collagen-sensitive mice

Kawalec-Carroll, Jill S.; Hetherington, Vincent J.; Dockery, Douglas W.; Shive, Carey L.; Targoni, Oleg S.; Lehmann, Paul; Nadler, Daniel; Prins, Dustin B.

doi:10.1186/1471-2474-7-32

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…It is well demonstrated that type II collagen, isolated, induced high titles of IL2, IL4, IFN-gama and specific IgG and IgM (Kawalec-Carroll et al 2007). Certainly due to the alterations that this protein exhibits after treatment, specific imunoglobulins against type II collagen had not been detected in the serum of rats that received frozen bone or bone submitted the photo-oxidation.…”

Section: Discussionmentioning

confidence: 94%

Effects of freezing on bone histological morphology

et al. 2008

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Allograft bone has been widely used for reconstruction of different portions of the skeleton. The fragment of bone harvested must be kept under low temperatures. The cryopreservation also contributes to decrease the antigenic potential of the tissue. Although this technique is considered safe, there is little information about the morphological modifications that the medullary and cortical portions of bone suffer after freezing. Hence, the aim of this study was to investigate the morphology of bone tissue after freezing under different temperatures and periods. Twelve rabbits were used to analyze the effects of two temperatures, -20 degrees C and -70 degrees C, during four periods of time: 30, 60, 90, 120 days. Tissues were analyzed by HE, picro-sirius stains and also by Feulgen's reaction, through qualitative and morphometric ways, considering the area occupied by cells and nuclei, medullary and cortical portions, as well as by collagen expression at cortical. The differences among the treatments were analyzed by Tukey s test, at 5% significance level. Bone freezing increased cellular and nuclear areas at cancellous bone and diminished nuclear area at the cortical bone. Cortical bone collagen suffered denaturation proportionally to temperature decrease and to freezing duration. These alterations compromised the morphology of tissues after 90 or 120 days of freezing at the temperature of -70 degrees C. Cells necrosed during freezing, contributing to reduce bone antigenicity.

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Section: Discussionmentioning

confidence: 94%

Effects of freezing on bone histological morphology

et al. 2008

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“…The main problem associated with the application of collagen and its derivatives as bone fillers comes from the intrinsic immunogenicity of the collagen molecule, 279 namely, most of it is xenogenic in origin because it is difficult to obtain directly from a patient, and recombinant technologies and the methods to extract the immunogenic, telopeptide portion of collagen molecules are not only of limited availability but also lead to reduced bioactivity of the protein. 280 Although collagen has been successfully applied topically, for example, in biodegradable sutures and as a prophylactic wound dressing carrier of antibiotics, 281–283 its mere subcutaneous epithelialization may lead to undesired immunogenic or antigenic responses.…”

Section: Advanced Drug Delivery Platforms In the Research Stagementioning

confidence: 99%

Nanostructured Platforms for the Sustained and Local Delivery of Antibiotics in the Treatment of Osteomyelitis

Uskoković

2015

Crit Rev Ther Drug Carrier Syst

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This article provides a critical view of the current state of the development of nanoparticulate and other solid-state carriers for the local delivery of antibiotics in the treatment of osteomyelitis. Mentioned are the downsides of traditional means for treating bone infection, which involve systemic administration of antibiotics and surgical debridement, along with the rather imperfect local delivery options currently available in the clinic. Envisaged are more sophisticated carriers for the local and sustained delivery of antimicrobials, including bioresorbable polymeric, collagenous, liquid crystalline, and bioglass- and nanotube-based carriers, as well as those composed of calcium phosphate, the mineral component of bone and teeth. A special emphasis is placed on composite multifunctional antibiotic carriers of a nanoparticulate nature and on their ability to induce osteogenesis of hard tissues demineralized due to disease. An ideal carrier of this type would prevent the long-term, repetitive, and systemic administration of antibiotics and either minimize or completely eliminate the need for surgical debridement of necrotic tissue. Potential problems faced by even hypothetically “perfect” antibiotic delivery vehicles are mentioned too, including (i) intracellular bacterial colonies involved in recurrent, chronic osteomyelitis; (ii) the need for mechanical and release properties to be adjusted to the area of surgical placement; (iii) different environments in which in vitro and in vivo testings are carried out; (iv) unpredictable synergies between drug delivery system components; and (v) experimental sensitivity issues entailing the increasing subtlety of the design of nanoplatforms for the controlled delivery of therapeutics.

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“…The second problem associated with the application of collagen as a component of bone fillers comes from its intrinsic immunogenicity 56 , a direct consequence of the fact that it is difficult to obtain directly from the patient and that most of it is xenogeneic in nature, while recombinant technologies as well as the methods to extract the immunogenic, telopeptide portion of collagen molecules are not only of limited availability, but also lead to reduced bioactivity of the protein 57 . The products of its degradation in vivo , the rate of which is often very variable, depending on the concentration of immunologically activated collagenases in the extracellular matrix surrounding the implant, have frequently been observed to lead to fibrous capsule formation 58 .…”

Section: Collagen/apatite Composites Modeled After the Structure Omentioning

confidence: 99%

When 1 + 1 > 2: Nanostructured composites for hard tissue engineering applications

Uskoković

2015

Materials Science and Engineering: C

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Multicomponent, synergistic and multifunctional nanostructures have taken over the spotlight in the realm of biomedical nanotechnologies. The most prospective materials for bone regeneration today are almost exclusively composites comprising two or more components that compensate for the shortcomings of each one of them alone. This is quite natural in view of the fact that all hard tissues in the human body, except perhaps the tooth enamel, are composite nanostructures. This review article highlights some of the most prospective breakthroughs made in this research direction, with the hard tissues in main focus being those comprising bone, tooth cementum, dentin and enamel. The major obstacles to creating collagen/apatite composites modeled after the structure of bone are mentioned, including the immunogenicity of xenogeneic collagen and continuously failing attempts to replicate the biomineralization process in vitro. Composites comprising a polymeric component and calcium phosphate are discussed in light of their ability to emulate the soft/hard composite structure of bone. Hard tissue engineering composites created using hard material components other than calcium phosphates, including silica, metals and several types of nanotubes, are also discoursed on, alongside additional components deliverable using these materials, such as cells, growth factors, peptides, antibiotics, antiresorptive and anabolic agents, pharmacokinetic conjugates and various cell-specific targeting moieties. It is concluded that a variety of hard tissue structures in the body necessitates a similar variety of biomaterials for their regeneration. The ongoing development of nanocomposites for bone restoration will result in smart, theranostic materials, capable of acting therapeutically in direct feedback with the outcome of in situ disease monitoring at the cellular and subcellular scales. Progress in this research direction is expected to take us to the next generation of biomaterials, designed with the purpose of fulfilling Daedalus’ dream - not restoring the tissues, but rather augmenting them.

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Immunogenicity of unprocessed and photooxidized bovine and human osteochondral grafts in collagen-sensitive mice

Cited by 7 publications

References 33 publications

Effects of freezing on bone histological morphology

Effects of freezing on bone histological morphology

Nanostructured Platforms for the Sustained and Local Delivery of Antibiotics in the Treatment of Osteomyelitis

When 1 + 1 > 2: Nanostructured composites for hard tissue engineering applications

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