Immunogenicity studies of various experimental vaccines in chickens

Vaillant, Angel Justiz; Akpaka, Patrick Eberechi

doi:10.1101/2020.08.31.276154

Cited by 2 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study emphasises the significance of investigating alternative immunotherapeutic strategies and highlights the potential of hyperimmune egg-derived antibodies as a treatment modality. Overall, the findings contribute to the understanding of HIV immunology and emphasize the importance of continued research into effective HIV treatment options [65,67,68] .…”

Section: An Hiv Experimental Vaccinesmentioning

confidence: 71%

Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas and Global Impact

Justiz-Vaillant,

Gopaul,

Soodeen

et al. 2024

Preprint

View full text Add to dashboard Cite

Immunology and microbiology research in the West Indies has witnessed remarkable growth and innovation, playing a pivotal role in advancing our understanding of immune mechanisms, disease pathogenesis, and therapeutic interventions. This manuscript presents a comprehensive exploration of key areas in West Indian immunology research, spanning from the utilization of bacterial proteins as antibody reagents to the intricate realms of clinical immunology and disease management. The utilization of bacterial immunoglobulin-binding proteins (IBPs), including protein A (SpA), protein G (SpG), and protein L (SpL), has revolutionised serological diagnostics in the region. promise in early disease detection and precision medicine. Microbiological studies in the Caribbean region have shed light on antimicrobial resistance patterns, particularly the emergence of extended-spectrum beta-lactamases (ESBLs), guiding antimicrobial stewardship programs and informing therapeutic strategies. Clinical immunology research has elucidated the molecular pathways underlying immune-mediated disorders, leading to tailored management strategies for conditions such as severe combined immunodeficiency (SCID), neuropsychiatric lupus erythematosus (NPLE), and others. In summary, West Indian immunology and microbiology research has made significant contributions to global healthcare, fostering collaboration, innovation, and improved patient outcomes.

show abstract

Section: An Hiv Experimental Vaccinesmentioning

confidence: 71%

Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas and Global Impact

Justiz-Vaillant,

Gopaul,

Soodeen

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Fragments 308-331 and 421-438 of the HIV gp120 [5] 2. Fragments 579-601 of the HIV gp 41 [5]. This peptide, derived from the V3 loop of HIV gp120, completely blocks the fusion inhibition activity of antisera to recombinant human HIV proteins and inhibits the infection of primary human T cells by HIV-1 as syncytium formation.…”

Section: Immunogenicity Studies Of Experimental Hiv Vaccine Candidates In Chickensmentioning

confidence: 99%

“…The feeding schedule was a blinding up process, the scientist did not take part in this process, and they did not know that each group was being treated. At the end of the feeding schedule, blood samples were obtained from all the chicks and investigated using an inhibition ELISA [5].…”

Section: Feeding Of Chicksmentioning

confidence: 99%

“…Fifteen grams of non-fat dry milk was added to complete the preparation of this solution, and 15 g of non-fat dry milk was added. The sample/conjugate diluent was prepared by adding 15 g of nonfat dry milk and 2.5 mL of 10% Tween 20 to 500 mL of PBS [5].…”

Section: Preparation Of Elisa Reagentsmentioning

confidence: 99%

“…After further incubation for 16 min in the dark, the reaction was stopped with a solution of 3M HCl, and each microplate was read in a microplate reader at 450 nm. The cut-off value was assessed based on the mean optical density (OD) of the negative control time of 2 [2][3][4][5]. The cut-off points of ELISAs for the detection of the anti-HIV peptide (579-601), anti-HIV peptide (308-331), and anti-HIV peptide (421-438) were 0.42, 0.40, and 0.44, respectively.…”

Section: Elisa For Anti-hiv Peptide Antibodiesmentioning

confidence: 99%

See 2 more Smart Citations

In vivoadministration of anti-HIV hyper-immune eggs induces anti-anti-idiotypic antibodies against HIV gp120 peptides (fragments 308-331 and 421-438) and against HIV gp41 peptide (fragment 579-601) which have demonstrable protective capacity

Justiz-Vaillant

Ferrer-Cosme²,

et al. 2021

Preprint

View full text Add to dashboard Cite

Isolation of antibodies from the egg yolk of chickens is of particular interest as a source of specific antibodies for oral administration to prevent infections and use them as immunodiagnostic reagents. The use of birds in antibody production results in a reduction in the use of laboratory animals. Immunized chickens produce larger quantities of antibodies (2000 mg IgY/month) than rodents (200 mg IgG/month) in the laboratory. According to Jerne’s network theory, it is possible to produce an antibody against the antigen-binding site of another antibody. This study assessed the hypothesis that immunization with viral peptides (immunogens) could provide a potent immune response that could be evaluated in chicken eggs. Human immunodeficiency virus 1(HIV-1) is used as an immunogen. The second hypothesis was that an orally administered antibody stimulates the production of a complementary antibody, the so-called anti-idiotypic antibody, which can potentially be therapeutical. This study reports and analyzes the use of eggs as therapeutic agents. We wanted to test the hypothesis that feeding chicks with hyperimmune eggs stimulates the production of anti-anti-idiotypic antibodies that neutralize the original HIV antigen fragments 308-331 or 421-438 of gp120 or fragment 579-601 of gp41. Future research could entail an anti-idiotype strategy for prophylactic vaccines. It is vital to note that it may need an anti-idiotype response to prime immunity against an HIV viral epitope, which may be used as a secondary element. The use of anti-idiotype immune responses in infected individuals may shift the balance of the immune system, allowing the organism to manage HIV infection. Therefore, it may be an avenue for immunotherapy to improve the fight against HIV infections. However, more studies and clinical trials are required to demonstrate similar human immune responses as observed in birds.

show abstract

Immunogenicity studies of various experimental vaccines in chickens

Cited by 2 publications

References 17 publications

Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas and Global Impact

Advancements in Immunology and Microbiology Research: A Comprehensive Exploration of Key Areas and Global Impact

In vivoadministration of anti-HIV hyper-immune eggs induces anti-anti-idiotypic antibodies against HIV gp120 peptides (fragments 308-331 and 421-438) and against HIV gp41 peptide (fragment 579-601) which have demonstrable protective capacity

Contact Info

Product

Resources

About