Immunoglobulin A-specific serodiagnosis of acute human cytomegalovirus infection by using recombinant viral antigens

Vornhagen, Rolf; Hinderer, Walter; Sonneborn, H.‐H.; Bein, Gregor; Matter, L; Jahn, Gerhard; Plachter, Bodo

doi:10.1128/jcm.34.4.1020-1023.1996

Cited by 6 publications

(1 citation statement)

References 9 publications

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“…Serial serum samples for initial evaluation originated from renal and hepatic allograft recipients, undergoing HCMV infection, and were obtained from the University Hospital, University of Groningen, Groningen, The Netherlands, and from the University Hospital, University of Lu È beck, Lu È beck, Germany [Vornhagen et al, 1996]. Sera were prede®ned according to anti-HCMV IgG serology as cases of primary or recurrent HCMV infections.…”

Section: Serum Samplesmentioning

confidence: 99%

An antigen fragment encompassing the AD2 domains of glycoprotein B from two different strains is sufficient for differentiation of primary vs. recurrent human cytomegalovirus infection by ELISA

Rothe

Pepperl-Klindworth²,

Lang

et al. 2001

Journal of Medical Virology

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Primary human cytomegalovirus (HCMV) infection during pregnancy is a frequent cause of fatal damage in populations with low prevalence of HCMV. Differentiation of primary vs. recurrent HCMV infection is an important issue in prenatal counseling. Antibodies specific for viral glycoproteins become detectable only with considerable delay with relation to HCMV infection or IgG seroconversion. Thus, lack of glycoprotein specific (gp-specific) antibodies can serve as a convenient indicator to identify those pregnant women that bear an elevated risk for HCMV transplacental transmission and fetal sequelae. In the opposite case, presence of gp-specific antibodies virtually excludes HCMV primary infection several weeks before sampling. However, no standardized screening assay for HCMV gp-specific antibodies had been available thus far. For this reason, an ELISA based on procaryotically expressed fragments of HCMV glycoprotein B (gB; gpUL55) was developed. Small fragments of gB from two different laboratory strains, encompassing the antigenic domain 2 (AD2) sufficed for sensitive and specific detection of gp-specific antibodies. The gB-ELISA titers correlated with titers of virus neutralizing antibodies in serum samples from primary or recurrent HCMV infections. Seroconversion kinetics of the gB-ELISA in samples from patients with primary HCMV infection closely paralleled the delay in seroconversion of gp-specific antibodies as determined by neutralization assay. Thus this assay provides a diagnostic tool that is easy to perform and can significantly add to available methods for the timely identification of primary HCMV infection during pregnancy. In addition, the gB-ELISA may be helpful in other clinical settings for the differentiation of primary HCMV infection from diseases caused by other pathogens.

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Section: Serum Samplesmentioning

confidence: 99%