Immunoglobulin and T cell receptor gene rearrangements in human lymphoma and leukemia

Abstract

DNA samples from blood leukocytes or tumor biopsies of 45 patients with phenotypic B or T cell neoplasms were analyzed for rearrangements of the immunoglobulin (Ig) or T cell receptor (TCR) genes by Southern blot hybridization analysis. Rearrangements of the Ig heavy chain joining region genes (JH) were present in DNA from each of 28 B cell lymphomas and leukemias; 14 of 21 of these tumors also had rearrangements of the Ig kappa light chain joining (JK) or deleting element (KDel) genes. Conversely, 16 of 17 T cell lymphomas and leukemias had rearranged TCR beta chain genes. One B cell and one T cell tumor had rearrangements of both Ig and TCR genes. There was a strong correlation between the rearrangements of specific genes and the immunophenotype of the tumor: JH rearrangement without TCR beta chain rearrangement occurred only in B cell tumors; TCR beta chain rearrangement with or without JH rearrangement occurred only in T cell tumors, with one exception; and JK and KDel rearrangements were found only in B cell tumors. Thus, rearrangements of the Ig heavy and light chain genes and the TCR beta chain genes were found to be highly sensitive markers of monoclonal human lymphomas and lymphoid leukemias, with the type of gene rearrangements well correlated with the cell lineage of these neoplasms.

Rearrangement of the chromosome 11 bcl-1 locus in centrocytic lymphoma: analysis with multiple breakpoint probes

“…multiple myeloma (14). and pre-B-cell acute lymphoblastic leukemia (9). One of 23 B-cell NHL of large cell type and one of 19 chronic lymphocytic leukemias or small lymphocytic NHL had MTC rearrangement.…”

BIOMED‐2 PCR assays for IGK gene rearrangements are essential for B‐cell clonality analysis in follicular lymphoma

“…This process implies that the IGK loci are rearranged in essentially all differentiated B‐cells. Using Southern blotting (Arnold et al , 1983; Cleary et al , 1984; Williams et al , 1987; Spencer et al , 1989) and polymerase chain reactions (PCR) (Bourguin et al , 1990; McCarthy et al , 1990; Trainor et al , 1990; Kuppers et al , 1993), it has been shown that both the rearranged IGH and IGK are highly sensitive and complementary markers in the detection of monoclonal B‐cell proliferations. However, in routine practice IGH is more commonly used than IGK for PCR‐based B‐cell clonality analysis, despite being known to give a high false negative rate in germinal/post‐germinal centre B‐cell malignancies (Gong et al , 1999; Diss et al , 2002; Pai et al , 2005; Amara et al , 2006).…”

Mechanism of selection of class II recombinant murine leukemia viruses in the highly leukemic strain CWD

“…Probes were prepared by digestion of the plasmids with the appropriate restriction enzymes and purification of the relevant fragments from low-melting-point agarose gels. The fragments were labeled with 32P by nick translation (41,44).…”

“…Southern blot assays. The technique for the isolation of DNAs from tissues and cells and the analysis of DNA fragments by the Southern blot assay were described previously (40,41,44). Autoradiograms were obtained by exposing Kodak XRP-5 film to the nylon membranes for 3 to 10 days at -70°C with the aid of intensifier screens.…”

“…The min'or' oligonucleotides marked by dashes were derived from the CWD endogenous ecotropic viruses (41). and CBA/J mice (14,(43)(44)(45). Although a target cell for PTV -1 must have been present in CWD mice, it was important to establish that the cellular phenotype of the CWD tumors was similar to that observed in the other strains.…”