Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging

Tsuboi, Setsuko; Sasaki, Akira; Sakata, Takao; Yasuda, Hidehiro

doi:10.1039/c7cc04966h

Cited by 18 publications

(18 citation statements)

References 51 publications

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“…Another concerning issue is that the QDs' surface capping agents cannot hinder the thiol binding by competing for the available surface sites. 42,43,49 The association of QDs and biomolecules by direct attachment to the nanocrystal surface has been widely used, for example with proteins, [50][51][52] carbodydrates, 25,53 polymers 54 and viruses. 55 In search of more efficient bimodal contrast agents for optical and magnetic resonance imaging (MRI), McAdams et al 37 anchored Gd III complexes on the QDs surface.…”

Section: Direct Attachment To the Surfacementioning

confidence: 99%

(Bio)conjugation Strategies Applied to Fluorescent Semiconductor Quantum Dots

Pereira¹,

Monteiro²,

Albuquerque³

et al. 2019

J. Braz. Chem. Soc.

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Quantum dots (QDs) are semiconductor nanocrystals, which present unique photophysical properties, enabling their application as new fluorescent platforms for biomedical sciences. Colloidal QDs are end-capped with organic or inorganic compounds, not only to prevent their agglomeration but also to provide reaction sites for the attachment of targeting (bio)molecules, nanoparticles or other interfaces, for specific biological purposes. The (bio)conjugation can involve non-covalent or covalent interactions, which can be accomplished through different strategies. The final assembly needs to maintain its chemical and optical stability and biochemical functionality. Although a relative good comprehension of the experimental procedures has been established, the bioconjugation process is still a challenge. The present manuscript aims to review the main (bio)conjugation strategies successfully applied to QDs, describing the steps necessary to prepare stable targeting fluorescent nanoplatforms, as well as some usual methods used to evaluate and optimize this process.

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Section: Direct Attachment To the Surfacementioning

confidence: 99%

(Bio)conjugation Strategies Applied to Fluorescent Semiconductor Quantum Dots

Pereira¹,

Monteiro²,

Albuquerque³

et al. 2019

J. Braz. Chem. Soc.

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“…For the conjugate of QD and His-RLuc-GB1 protein, BRET efficiency (E) was estimated to about 63%, which was calculated from the following equation: 48,49 where I DA and I D represent the emission intensity of CTZ in the presence and absence of acceptor (QD), respectively. It should be noted that NIR luminescence at 700-1000 nm weakly emitted for the mixture of QD/RLuc-GB1.…”

Section: Bret Between Ctz and Qdmentioning

confidence: 99%

BRET based dual-colour (visible/near-infrared) molecular imaging using a quantum dot/EGFP–luciferase conjugate

Tsuboi

2019

RSC Adv.

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“…Quantum dots have been recently developed as new fluorescent nanomaterials for biological labeling applications [1,2] . These nanomaterials have unique optical properties such as photo stability, high fluorescence intensity, and low level of fluorescence spectral overlap [3][4][5] . In biological labeling, QDs are commonly conjugated to antibody in order to direct them to antigen [6] .…”

Section: Introductionmentioning

confidence: 99%

Application of Antibody-Conjugated CdSe/MSA Quantum Dots on Immunohistochemistry

Tran¹,

Khanh-Thien²,

Mai-Hoang³

et al. 2020

SSR-IIJLS

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Background: Quantum dots (QDs) are recently conjugated to antibody for using in biological labeling applications. In previous studies, we developed CdSe/MSA QDs, which were coated with protein A/G (pA/G) for antibody conjugation, and evaluated their cell staining application. Here, we expanded their applications into immunohistochemistry (IHC) by investigating their storage time by accelerated heat aging method, and comparing them with FITC in sample labeling. Methods: Cytokeratin 6A (KRT6A) in animal skin tissue section were stained by pA/G-coated QDs conjugated to anti-KRT6A antibody and TF-1 human erythroleukemia cells were stained by pA/G-coated QDs conjugated to anti-CD34 antibody. Results: The results indicated that our pA/G-coated QDs effectively stained KRT6A in epidermis of skin tissue section when they were probed with specific antibody. Besides, pA/G-coated QDs still maintained their labeling ability in cell staining and IHC formats after 12-month storage at 4 o C. In comparison with FITC (relatively almost the same emission fluorophore), our QDs showed a significantly stronger fluorescent signal. Conclusion:In conclusion, these results indicated that our pA/G-coated QDs were potentially fluorescent nanomaterials and could be applied in a wide range of biological labeling applications.

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Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging

Abstract: A facile method for the preparation of antibody-quantum dot (QD) conjugates using the immunoglobulin binding (B1) domain of protein G is presented. The utility of antibody-QD conjugates using the B1 domain is demonstrated for fluorescence imaging of breast tumor cells in vitro and in vivo.

Cited by 18 publications

References 51 publications

(Bio)conjugation Strategies Applied to Fluorescent Semiconductor Quantum Dots

(Bio)conjugation Strategies Applied to Fluorescent Semiconductor Quantum Dots

BRET based dual-colour (visible/near-infrared) molecular imaging using a quantum dot/EGFP–luciferase conjugate

Application of Antibody-Conjugated CdSe/MSA Quantum Dots on Immunohistochemistry

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