Immunoglobulin Concentration and Specific Antibody Activity in Oocytes and Eggs of Immunized Red Sea Bream

Abstract

The IgM concentration and specific antibody titre of oocyte and egg extracts of Vibrio-immunized red sea bream were successfully measured by an enzyme immunoassay and an ELISA procedure. The concentration of oocytic immunoglobulin was found to increase during the vitellogenesis process. The oocytes as well as the fertilized eggs of the immunized females had antibody titre much higher than that of the control group. On the other hand, no considerable difference in immunoglobulin concentration was found between the eggs obtained from immunized and control mothers. Hence, the comparison of the value of antibody titre per mg IgM estimated for the maternal blood, oocytes and eggs of the same fish showed that common features of immunity exist between the oocytes, eggs and maternal blood. The possible origin of the oocytic and egg IgM was shown to be the maternal blood plasma.

Transfer of maternally-derived immunoglobulin (IgM) to larvae in tilapia,Oreochromis mossambicus

“…Similar results have been reported in other teleost fish (Bly et al, 1986;Mor & Avtalion, 1988;Kanlis et al, 1995a,b;Lillehaug et al, 1996). A correlation between the antibody titres of oocytes/eggs and the maternal blood has been suggested in red sea bream, Pagrus major, (Kanlis et al, 1995b); high antibody titre in the maternal blood coincided with high titres in oocytes and eggs. It has also been suggested that accumulation of antibody occurs during vitellogenesis (Mor & Avtalion, 1990;Kanlis et al, 1995b).…”

“…A correlation between the antibody titres of oocytes/eggs and the maternal blood has been suggested in red sea bream, Pagrus major, (Kanlis et al, 1995b); high antibody titre in the maternal blood coincided with high titres in oocytes and eggs. It has also been suggested that accumulation of antibody occurs during vitellogenesis (Mor & Avtalion, 1990;Kanlis et al, 1995b). In addition to the eggs, antibody activity against BSA could be detected in the medium bathing the tail-cut prelarvae obtained from the immunised mother in the present study.…”

The Effects of Letrozole in Transfer of Maternal Immunity against Lactococcosis to Eggs and Larvae in Rainbow Trout (<i>Oncorhynchus mykiss</i>, Walbaum)

“…Sin et al [40] reported significant antibody against Ichthtyophinus multifilis in Oreochromis aureus by immunising 1 month prior to spawning and also re- ported successful transfer of antibody from mother to offspring. The successful transfer of immunoglobulin antibody from mother to offspring has also earlier been reported in many fish species including plaice [41], tilapia [42]- [44], red sea bream [45] and sea bream [46]. In the present study, the high levels of circulating antibodies detected against L. garvieae confirmed the immune response of the parents.…”

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“…Although, the variations of the immune parameters such as the lysozyme, C3 and IgM in the larval stages of some teleost species have been studied, there is no such information for the sturgeons. The existence of maternal lysozyme has recently been confirmed in the oocytes, fertilized eggs and larval stages of Coho salmon (Oncorhynchus kisutch) (Yousif, Albright, & Evelyn, 1994), red sea bream (Pagrus major) (Kanlis, Suzuki, Tauchi, Numata, & Shirojo, 1995) and tilapia (Oreochromis niloticus) (Sin, Ling, & Lam, 1994). It had been shown that the lysozyme in the unfertilized eggs had higher level than the fertilized eggs (1 HPF) and 6 DPH larvae (Cecchini, Terova, Caricato, & Saroglia, 2000).…”

“…The maternal transfer of the IgM to the embryo has been demonstrated in several species such as plaice (Bly, Grimm, & Morris, 1986), tilapia (Takemura & Takano, 1997) and red sea bream (Kanlis et al, 1995). In sea bass (Dicentrarchus labrax), for example, the maternal IgM had been deposited in the ovary during vitellogenesis and reached a maximum concentration during ovulation.…”