Immunoglobulin deposition on biomolecule corona determines complement opsonization efficiency of preclinical and clinical nanoparticles

Vu, Vivian; Gifford, Geoffrey; Chen, Fangfang; Benasutti, Halli; Wang, Guankui; Groman, Ernest; Scheinman, Robert I.; Saba, Laura; Moghimi, S. Moein; Simberg, Dmitri

doi:10.1038/s41565-018-0344-3

Cited by 237 publications

(237 citation statements)

References 49 publications

Supporting

Mentioning

233

Contrasting

Order By: Relevance

“…In another study, it was shown that clinical and preclinical NPs are recognized by “natural” pre-existing antibodies and that labeling of NP-bound IgG by C3b/iC3b opsonins is crucial for an effective capture by phagocytes ( 69 ). The use of FcR-negative or overexpressing cell lines also indirectly suggested that NP-bound antibodies mediate cell interaction with phagocytes ( 41 , 70 ).…”

Section: Influence On Np-cell Interactions Of Specific Np-bound Protementioning

confidence: 99%

Opsonins and Dysopsonins of Nanoparticles: Facts, Concepts, and Methodological Guidelines

2020

View full text Add to dashboard Cite

Understanding the effects mediated by a set of nanoparticle (NP)-bound host biomolecules, often indicated with the umbrella term of NP corona , is essential in nanomedicine, nanopharmacology, and nanotoxicology. Among the NP-adsorbed proteome , some factors mediate cell binding, endocytosis, and clearing by macrophages and other phagocytes (opsonins), while some others display few affinities for the cell surface (dysopsonins). The functional mapping of opsonins and dysopsonins is instrumental to design long-circulating and nanotoxicologically safe next-generation nanotheranostics. In this review, we critically analyze functional data identifying specific proteins with opsonin or dysopsonin properties. Special attention is dedicated to the following: (1) the simplicity or complexity of the NP proteome and its modulation, (2) the role of specific host proteins in mediating the stealth properties of uncoated or polymer-coated NPs, and (3) the ability of the innate immune system, and, in particular, of the complement proteins, to mediate NP clearance by phagocytes. Emerging species-specific peculiarities, differentiating humans from preclinical animal models (the murine especially), are highlighted throughout this overview. The operative definition of opsonin and dysopsonin and the measurement schemes to assess their in vitro efficacy is critically re-examined. This provides a shared and unbiased approach useful for NP opsonin and dysopsonin systematic identification.

show abstract

Section: Influence On Np-cell Interactions Of Specific Np-bound Protementioning

confidence: 99%

Opsonins and Dysopsonins of Nanoparticles: Facts, Concepts, and Methodological Guidelines

2020

View full text Add to dashboard Cite

show abstract

“…Upon exposure to biofluids, the NPs acquire a "protein corona" due to the adherence of host proteins on the NPs surface. The composition of the corona is dependent on the types of nanoparticles and the biological sources [1][2][3] , and considered to provide the NPs with a "biological" identity 4 that affects stability, circulation time, and cellular uptake/interactions, and therefore has a strong impact on the functional role for the NPs [5][6][7][8][9][10] .…”

mentioning

confidence: 99%

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

et al. 2020

View full text Add to dashboard Cite

The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

show abstract

“…Indeed, one proposed mechanism of ENM-induced complement activation and C3 opsonization is attributed to subsequent conformational changes of ENM-adsorbed biomacromolecules (i.e., exposing antigenic epitopes) (46). Further, it has been recently shown that natural antibodies play a key role in ENM-induced complement opsonization (47). Importantly, such mechanisms appears to be universal to multiple preclinical and clinical nanomedicines (47).…”

Section: Interaction With and Activation Of The Complement Systemmentioning

confidence: 99%

“…Further, it has been recently shown that natural antibodies play a key role in ENM-induced complement opsonization (47). Importantly, such mechanisms appears to be universal to multiple preclinical and clinical nanomedicines (47). Taken together, due to ENM size and physicochemical properties (e.g., shape, surface charge, topography, hydrophobicity, etc.…”

Section: Interaction With and Activation Of The Complement Systemmentioning

confidence: 99%

Engineered Nanomaterials and Type I Allergic Hypersensitivity Reactions

Alsaleh

Brown

2020

Front. Immunol.

View full text Add to dashboard Cite

Type I allergic hypersensitivity disorders (atopy) including asthma, atopic dermatitis, allergic rhinitis, and food allergy are on the rise in developed and developing countries. Engineered nanomaterials (ENMs) span a large spectrum of material compositions including carbonic, metals, polymers, lipid-based, proteins, and peptides and are being utilized in a wide range of industries including healthcare and pharmaceuticals, electronics, construction, and food industry, and yet, regulations for the use of ENMs in consumer products are largely lacking. Prior evidence has demonstrated the potential of ENMs to induce and/or aggravate type I allergic hypersensitivity responses. Furthermore, previous studies have shown that ENMs could directly interact with and activate key T-helper 2 (Th2) effector cell types (such as mast cells) and the complement system, which could result in pseudoallergic (non-IgE-mediated) hypersensitivity reactions. Nevertheless, the underlying molecular mechanisms of ENM-mediated induction and/or exacerbation of type I immune responses are poorly understood. In this review, we first highlight key examples of studies that have demonstrated inherent immunomodulatory properties of ENMs in the context of type I allergic hypersensitivity reactions, and most importantly, we attempt to put together the potential molecular mechanisms that could drive ENM-mediated stimulation and/or aggravation of type I allergic hypersensitivity responses.

show abstract

Immunoglobulin deposition on biomolecule corona determines complement opsonization efficiency of preclinical and clinical nanoparticles

Cited by 237 publications

References 49 publications

Opsonins and Dysopsonins of Nanoparticles: Facts, Concepts, and Methodological Guidelines

Opsonins and Dysopsonins of Nanoparticles: Facts, Concepts, and Methodological Guidelines

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Engineered Nanomaterials and Type I Allergic Hypersensitivity Reactions

Contact Info

Product

Resources

About