Immunoglobulin G Subclass Profile of Antimeasles Response in Vaccinated Children and in Adults with Measles History

Abstract

The aim of this study was to investigate measles-specific immunoglobulin G (IgG) subclass profile in vaccinated children and in adults with natural infection. Serum samples were collected before and 30 days after vaccination. The sera from 51 late convalescent adults and seven adults with natural measles infection at the 12th day after onset of rash have been also investigated. Measles IgG antibodies and specific IgG subclasses were tested by enzyme-linked immunosorbent techniques. In children younger than 3 years, the predominant subclass was IgG3, which contributed, on average, 63.3% of the total IgG response. The contributions of specific IgG1 and IgG4 to the total IgG antimeasles response were lower (19.9% and 16.8%, respectively), whereas IgG2 was not found. In contrast, in the group of children older than 4 years, just IgG2 was a predominant subclass; it contributed 42.6% of the total IgG response. Other subclasses were also present but the contribution was much lower. In adult volunteers with measles history, IgG2 was a predominant subclass of total IgG. Thus, in early convalescence IgG2 contributed 62% of the total IgG response, whereas in late convalescence the contribution was lower (41.4%). There were no visible differences in IgG subclass composition between subjects with natural infection and vaccinated children except those below 3 years of age. The humoral immune response of such subjects is immature and the IgG2 subclass of virus-specific antibodies has not been revealed in the sera.

Prevalence of Serum IgG and Neutralizing Factors Against Adeno-Associated Virus (AAV) Types 1, 2, 5, 6, 8, and 9 in the Healthy Population: Implications for Gene Therapy Using AAV Vectors

“…IgG1 was expected, as the subclass is commonly induced after viral infections such as measles, hepatitis B, human T-lymphotropic virus, rubella, and B19V, which like AAV is a member of the Parvoviridae (Morgan-Capner and Thomas, 1988;Lal et al, 1993;Franssila et al, 1996;Rey et al, 2000;Toptygina et al, 2005). IgG2 was also shown to be a constituent of the AAV-specific antibody response (Madsen et al, 2009;Murphy et al, 2009), but it is low as for B19V (Lal et al, 1993;Franssila et al, 1996;Toptygina et al, 2005).…”

Age‐Dependent Differences in IgG Isotype and Avidity Induced by Measles Vaccine Received during the First Year of Life

“…Although increased levels of Th2 cytokines such as IL-4 and IL-10 were not observed in response to measles vaccination in infants [11], IFN-g responses may be insufficient to induce a complete deployment of the Th1 response. The isotypes of MV-specific antibodies have been previously studied after immunization and natural infection [32][33][34][35][36]. These studies show that, in general, IgG3 is detected early and associated with decreasing levels over time, whereas levels of IgG1 continue to increase; IgG2 is rarely observed.…”

Autism: Maternally derived antibodies specific for fetal brain proteins

“…As circulating antibody titers are known to vary over time based on the immunological state of the individual (Toptygina et al, 2005), the maternal antibody profile observed at the time of the registration of her child into the CHARGE study may be slightly different than during gestation. However, it has been demonstrated that antibodies, such as those generated in response to vaccination, can persist for many years due to the maintenance and subsequent polyclonal reactivation of memory B cells (Shinefield et al, 2002).…”