Immunoglobulin isotype distribution of locally produced autoantibodies to collagen type I in adult periodontitis

Abstract

Production of antibodies to collagen type I was analyzed by means of an enzyme-linked immunospot (ELISPOT) assay in patients with chronic adult periodontitis (AP) before and after periodontal hygiene treatment. Anti-collagen type I antibody-secreting cells were found among mononuclear cells enzymatically eluted from inflamed gingiva in 9 of 15 patients with untreated AP and in 4 of 14 hygiene-treated patients with a varied isotype distribution. A notably high prevalence of IgG and IgM isotypes was observed for the anti-collagen antibodies in untreated patients. With wide variation, chronic AP was characterized by a high frequency of spontaneous IgG and low numbers of IgA and IgM-producing cells. Periodontal hygiene treatment significantly reduced the number of IgA and IgM-secreting cells. Although AP is not an autoimmune disease in the accepted sense, our results indicate that local autoimmune reactions to collagen type I are common in untreated AP, implying an interplay between periodontal infection and autoimmunity.

Immunoglobulin G and A Antibody Responses toBacteroides forsythusandPrevotella intermediain Sera and Synovial Fluids of Arthritis Patients

“…It is suggested that while T lymphocytes and polymorphonuclear cells dominate in the early stages of plaque accumulation and gingivitis, B cells dominate in more advanced lesions, with a higher degree of soft tissue destruction and bone loss (9,36). As is seen in periodontal disease (5,15,20,36), B cells are activated in the synovial tissue in patients with arthritis, leading to a spontaneous secretion of Igs (IgG, IgA, and IgM) (1). This lymphocytic response may lead to the subsequent formation of immune complexes, which are capable of inducing a spectrum of inflammatory mediators (17,34).…”

The Immune Modulation of B‐Cell Responses by Porphyromonas gingivalis and Interleukin‐10

“…Anti-collagen antibody has been consistently detected and suggested to be involved in gingival tissue destruction. 11,12,37 In addition to their well-known role in antibody production, B cells also have the determinative role as antigen-presenting cells (APCs) in the generation of T-cell-mediated immune responses. 38 Recent studies have shown that B cells are critical in the pathogenesis of T-cell-mediated autoimmune diseases.…”

The Sp1 transcription factor binds to the G-allele of the –1087 IL-10 gene polymorphism and enhances transcriptional activation

“…15,16 In addition, several studies reported on higher levels of antibodies to collagen type 1 in gingival tissue than in serum. [17][18][19] Several factor-binding sites that might be involved in the regulation of IL-10 were identified in the IL-10 promoter. [20][21][22] The IL-10 promoter contains several single nucleotide polymorphisms (SNPs) among which the -1087 G/A SNP (sometimes referred to as À1082 G/A) was associated with differences in IL-10 promoter activity and IL-10 production.…”