Immunoglobulin VH clan and family identity predicts variable domain structure and may influence antigen binding.

Kirkham, Perry M.; Mortari, Frank; Newton, J. A.; Schroeder, Harry W.

doi:10.1002/j.1460-2075.1992.tb05092.x

Cited by 174 publications

(83 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By contrast, the phylogenetic classification of tetrapod IGHV sequences into three clans (I, II, and III) is clearly supported by high (>75%) bootstrap values. These three clans are equivalent to the clans I, II, and III reported previously for the phylogenetic classification of mammalian IGHV sequences (Kirkham et al 1992;Schroeder et al 1990). The presence of all three clans in the frog Xenopus (Fig.…”

Section: Phylogenetic Relationships Of Ighv Genes In Vertebratessupporting

confidence: 78%

See 1 more Smart Citation

Evolutionary dynamics of the immunoglobulin heavy chain variable region genes in vertebrates

et al. 2008

View full text Add to dashboard Cite

Immunoglobulin heavy chains are polypeptides encoded by four genes: variable (IGHV), joining (IGHJ), diversity (IGHD), and constant (IGHC) region genes. The number of IGHV genes varies from species to species. To understand the evolution of the IGHV multigene family, we identified and analyzed the IGHV sequences from 16 vertebrate species. The results show that the numbers of functional and nonfunctional IGHV genes among different species are positively correlated. The number of IGHV genes is relatively stable in teleosts, but the intragenomic sequence variation is generally higher in teleosts than in tetrapods. The IGHV genes in tetrapods can be classified into three phylogenetic clans (I, II, and III). The clan III and/or II genes are relatively abundant, whereas clan I genes exist in small numbers or are absent in most species. The genomic organization of clan I, II, and III IGHV genes varies considerably among species, but the entire IGHV locus seems to be conserved in the subtelomeric or near-centromeric region of chromosome. The presence or absence of specific IGHV clan members and the lineage-specific expansion and contraction of IGHV genes indicate that the IGHV locus continues to evolve in a species-specific manner. Our results suggest that the evolution of IGHV multigene family is more complex than previously thought and that several factors may act synergistically for the development of antibody repertoire.

show abstract

Section: Phylogenetic Relationships Of Ighv Genes In Vertebratessupporting

confidence: 78%

“…On the basis of the degree of sequence identity, mammalian IGHV genes have been classified into three major clans (clans I-III) (Kirkham et al 1992;Kodaira et al 1986;Kofler et al 1992;Ota and Nei 1994;Schroeder et al 1990). The number of IGHV genes in these three clans varies among different mammals (Sitnikova and Su 1998).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary dynamics of the immunoglobulin heavy chain variable region genes in vertebrates

et al. 2008

View full text Add to dashboard Cite

show abstract

“…During this stage of differentiation, interactions with self antigens could lead to positive or negative selection of B cells expressing specific V H germline gene products (4,5). As pairing of H chains with L chain is thought to mask availability of the CDR3 (6), selection at this stage of B cell maturation is proposed to relate largely to the nature of the V H segment expressed. In the mouse, selection of the Ig repertoire based on V H expression has been suggested to occur (7).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

Brezinschek¹,

Foster²,

Brezinschek³

et al. 1997

J. Clin. Invest.

326

291

View full text Add to dashboard Cite

To analyze the immunoglobulin repertoire of human IgM ϩ B cells and the CD5ϩ and CD5 Ϫ subsets, individual CD19 ϩ / IgM ϩ /CD5 ϩ or CD5 Ϫ B cells were sorted and non-productive as well as productive V H gene rearrangements were amplified from genomic DNA and sequenced. In both subsets, the V H 3 family was overrepresented largely as a result of preferential usage of a small number of specific individual family members. In the CD5 ϩ B cell subset, all other V H families were found at a frequency expected from random usage, whereas in the CD5 Ϫ population, V H 4 appeared to be overrepresented in the nonproductive repertoire, and also negatively selected since it was found significantly less often in the productive compared to the nonproductive repertoire; the V H 1 family was significantly diminished in the productive rearrangements of CD5 Ϫ B cells. 3-23/DP-47 was the most frequently used V H gene segment and was found significantly more often than expected from random usage in productive rearrangements of both CD5

show abstract

“…This finding, together with the observation that fish V H sequences have their closest homologs in mammalian clan III sequences, led to the assumption that this is the most ancient of the three mammalian clans (5,(12)(13)(14)(15). However, more recent data have shown that both cow (Bos taurus) and sheep express only one V H gene family, which belongs to clan II (16 -19).…”

mentioning

confidence: 99%

Heavy Chain V Region Diversity in the Duck-Billed Platypus (Ornithorhynchus anatinus): Long and Highly Variable Complementarity-Determining Region 3 Compensates for Limited Germline Diversity

Johansson

Aveskogh

Munday

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

In this work, to study the emergence of the H chain V region repertoire during mammalian evolution, we present an analysis of 25 independent H chain V regions from a monotreme, the Australian duck-billed platypus, Ornithorhynchus anatinus. All the sequences analyzed were found to form a single branch within the clan III of mammalian V region sequences in a distance tree. However, compared with a classical V gene family this branch was more diversified in sequence. Sequence analysis indicates that the apparent lack of diversity in germline V segments is well compensated for by relatively long and highly diversified D and N nucleotides. In addition, extensive sequence variation was observed in the framework region 3. Furthermore, at least five and possibly seven different J segments seem to be actively used in recombination. Interestingly, internal cysteine bridges in the complementarity-determining region (CDR)3 loop, or between the CDR2 and CDR3 loops, are found in ∼36% of the platypus VH sequences. Such cysteine bridges have also been observed in cow, camel, and shark. Internal cysteine bridges may play a role in stabilizing long and diversified CDR3 and thereby have a role in increasing the affinity of the Ab-Ag interaction.

show abstract

Immunoglobulin VH clan and family identity predicts variable domain structure and may influence antigen binding.

Cited by 174 publications

References 34 publications

Evolutionary dynamics of the immunoglobulin heavy chain variable region genes in vertebrates

Evolutionary dynamics of the immunoglobulin heavy chain variable region genes in vertebrates

Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

Heavy Chain V Region Diversity in the Duck-Billed Platypus (Ornithorhynchus anatinus): Long and Highly Variable Complementarity-Determining Region 3 Compensates for Limited Germline Diversity

Contact Info

Product

Resources

About