Immunoglobulins G of Patients with Schizophrenia Protects from Superoxide: Pilot Results

Mednova, Irina A.; Smirnova, L.; Vasilieva, Alisa R.; Kazantseva, Daria V.; Epimakhova, Elena V.; Krotenko, N. M.; Semke, A.; Ivanova, Svetlana A.

doi:10.3390/jpm12091449

Cited by 5 publications

(3 citation statements)

References 46 publications

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“…SOD activity of IgG was expressed in the following units: the difference in optical density between the spontaneous and induced reaction proceeding for 1 min in terms of mg of IgG (µM diformazan/min/mg IgG) [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…IgG SOD activity has been found in schizophrenia and multiple sclerosis, but the function of abzymes having antioxidant activity is not fully understood. Over the past few years, abzymes with nuclease [ 17 , 18 ], proteolytic [ 19 , 20 ], catalase [ 21 ], and superoxide dismutase (SOD) activities [ 22 ] have been found in the blood serum of patients with schizophrenia. One hypothesis about the pathogenesis suggests that catalytic antibodies with catalase and SOD activities help protect the body from excessive concentrations of reactive oxygen species (ROS) [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Different Directions of Effects of Polyclonal IgG Antibodies from Patients with Schizophrenia and Healthy Individuals on Cell Death In Vitro: A Pilot Study

Epimakhova

Smirnova

Kazantseva

et al. 2023

CIMB

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Numerous studies indicate the involvemen of oxidative stress in the pathogenesis of schizophrenia. It has been shown that the serum pool of antibodies in patients with schizophrenia contains catalytically active antibodies (abzymes) that have a wide range of activities, including redox properties. In the present work, the effects of IgGs—having oxidoreductase activities—isolated from the serum of patients with schizophrenia and healthy individuals were studied in vitro. The IgGs were purified by affinity chromatography followed by an SDS-PAGE analysis of homogeneity in a 4–18% gradient gel. The catalase and superoxide dismutase (SOD) activities of the IgGs were measured spectrophotometrically using a kinetic module. Human neuroblastoma SH-SY5Y cells were cultured with IgG at a final concentration of 0.2 mg/mL for 24 h. In a parallel experiment, tert-butyl hydroperoxide was used as an oxidative stressor. The number of dead cells after incubation was determined with fluorescent dyes, propidium iodide and Hoechst, by high-throughput screening on the CellInsight CX7 platform. A cytotoxic effect of the IgG from the schizophrenia patients on SH-SY5Y cells was detected after 24 h incubation. A correlation was found between the SOD activity of the IgGs and IgG-induced cell death. Under the induced oxidative stress, the cytotoxic effect of the IgG from the patients with schizophrenia on the SH-SY5Y cell line was five times stronger. Meanwhile, the IgG from the healthy individuals exerted a cytoprotective effect on the cultured cells, accompanied by high catalase activity. Thus, the observed influence on cell viability depends on the catalytic properties of the abzymes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Different Directions of Effects of Polyclonal IgG Antibodies from Patients with Schizophrenia and Healthy Individuals on Cell Death In Vitro: A Pilot Study

Epimakhova

Smirnova

Kazantseva

et al. 2023

CIMB

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“…Over the past few years, group of the researchers at the Mental Health Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences (Tomsk, Russia) in collaboration with The Institute of Chemical Biology and Fundamental Medicine of Siberian Division of Russian Academy of Sciences (Novosibirsk, Russia) has investigated a number of catalytic activities of immunoglobulins G in patients with schizophrenia. They reported that abzymes isolated from the blood serum of patients exhibited catalase activity ( Ermakov et al, 2017 ), superoxide dismutase activity ( Mednova et al, 2022 ), nuclease activity ( Ermakov et al, 2015 ; Ermakov et al, 2018 ) and proteolytic activity to MBP ( Parshukova et al, 2020b ; Parshukova et al, 2019 ), histones ( Ermakov et al, 2020 ), and collagen ( Parshukova et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%

Some structural features of the peptide profile of myelin basic protein-hydrolyzing antibodies in schizophrenic patients

Zavialova

Parshukova

Kazieva

et al. 2023

PeerJ

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The antibodies of schizophrenic patients that hydrolyze myelin basic protein (MBP) have been actively studied recently, but the mechanism of the catalytic properties of immunoglobulin molecules remains unknown. Determination of specific immunoglobulin sequences associated with the high activity of MBP proteolysis will help to understand the mechanisms of abzyme catalysis. In the course of comparative mass spectrometric analysis of IgG peptides from the blood serum of patients with acute schizophrenia and healthy people, 12 sequences were identified, which were found only in antibodies that hydrolyze MBP. These sequences belong to IgG heavy chains and κ- and λ-type light chains, with eight of them belonging to variable domains. The content of peptides from the variable regions of the light chains does not correlate with the proteolytic activity of IgG to MBP in patients with schizophrenia, whereas for two sequences from the variable regions of the heavy chains (FQ(+0.98)GWVTMTR and *LYLQMN(+0.98)SLR), an increase in activity with increasing their concentration. The results suggest that these sequences may be involved in one way or another in MBP hydrolysis.

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Catalase Activity of IgG and κκ-IgG, λλ-IgG, and κλ-IgG Subfractions in HIV-Infected Patients and Healthy Donors

Tolmacheva,

Sedykh,

Onvumere

et al. 2024

Front. Biosci. (Landmark Ed)

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Background: Human immunodeficiency virus (HIV) infection is associated with pronounced oxidative stress, leading to the development of various virus-associated pathologies. A wealth of evidence suggests that, along with canonical enzymes of reactive oxygen species regulation, human blood contains antibodies with peroxidase, superoxide dismutase, and catalase activities. Here we show that the catalase activity of IgGs and their κκ-IgG, λλ-IgG, and κλ-IgG subfractions of HIV-infected individuals is significantly different compared to the healthy donors. Methods: Protein G-Sepharose sorbent was used to resolve IgG from blood of healthy donors and HIV-infected patients by affinity chromatography. Subfractions of κκ-IgG, λλ-IgG, and κλ-IgG were separated from IgGs samples of each group by affinity chromatography on sorbents containing immobilized antibodies to κ or λ light human chains. The IgG catalase activity level was measured spectrophotometrically by evaluating the decrease in optical density (A240) due to hydrogen peroxide decomposition. Results: The relative catalase activity of antibodies from HIV-infected patients (kcat = (1.41 ± 0.92) × 103 min-1, 95% CI: [1.01–1.81]) was statistically significant, 1.6 times higher (p = 0.014) compared to apparently healthy donors ((0.86 ± 0.49) × 103, 95% CI: [0.69–1.03]). The activity level of κκ-IgG HIV-infected patients ((0.44 ± 0.04) × 103 min-1) was 1.4 times higher than that of λλ-IgGs ((0.31 ± 0.025) × 103 min-1); the opposite was observed for κκ-IgGs from apparently healthy donors, which activity ((0.17 ± 0.015) × 103 min-1) was 3.1 times lower compared to λλ-IgGs ((0.53 ± 0.045) × 103 min-1). Conclusions: Thus, the data obtained may indicate that IgG with increased catalase activity may prevent harmful processes arising from oxidative stress in HIV-infected patients, acting as an additional natural molecular mechanism of regulation of hydrogen peroxide level.

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Immunoglobulins G of Patients with Schizophrenia Protects from Superoxide: Pilot Results

Cited by 5 publications

References 46 publications

Different Directions of Effects of Polyclonal IgG Antibodies from Patients with Schizophrenia and Healthy Individuals on Cell Death In Vitro: A Pilot Study

Different Directions of Effects of Polyclonal IgG Antibodies from Patients with Schizophrenia and Healthy Individuals on Cell Death In Vitro: A Pilot Study

Some structural features of the peptide profile of myelin basic protein-hydrolyzing antibodies in schizophrenic patients

Catalase Activity of IgG and κκ-IgG, λλ-IgG, and κλ-IgG Subfractions in HIV-Infected Patients and Healthy Donors

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