Immunohistochemical Analysis of Biodentine Versus MTA in Repair of Experimental Furcation Perforation

Abstract

This study compared the Biodentine (BD) and MTA in repair of furcation perforation (FP) in a dog model in terms of inflammatory cell count and new hard tissue formation through histological and immunohistochemical evaluations. Ninety six teeth in six adult mongrel dogs were divided according to the time of repair into two groups (48 teeth/3 dogs each); immediate and delayed repair of the induced FP. Each group was divided into three subgroups (16 teeth each) according to the evaluation periods; 1, 2, and 3 months. Each subgroup was further subdivided into four subdivisions according to the material used; MTA (6 teeth), Biodentine (6 teeth), negative control (2 teeth) and positive control (2 teeth). In experimental and positive control samples, the teeth were instrumented and obturated then, a FP was performed. The perforations were sealed according to the groups and subdivisions. Histopathology and immunohistochemical analysis using Osteonectin antibodies were performed for assessment of the inflammatory cell count and new hard tissue formation. In all groups and subgroups, there were no significant differences between MTA and BD in the inflammatory cell count and new hard tissue formation (P>0.05). Biodentine can alternate the MTA as a FP repair material.

Cytotoxic Effect of Tideglusib on Human Fibroblasts: An In Vitro Study

“…To enable adequate healing of exposed dental pulp, the pulp capping material must be bioactive, biocompatible, and antimicrobially active against a variety of microorganisms. 1,4,9 Different pulp capping materials have been studied in the literature, including calcium hydroxide, mineral trioxide aggregate, and other calcium silicate-based compounds. 1,4,10,11 Various studies have shown that calcium hydroxide improves healing and reparative dentin production.…”

“…12 Other research recommended using mineral trioxide aggregate (MTA) because it has a superior sealing ability and improves dentinal bridge quality. 9,11,14 MTA disadvantages, such as lengthy setting time and probable discoloration, prompted the development of additional calcium silicatebased polymers, such as biodentine, to address these shortcomings and increase physiochemical qualities. 7,8,14 Tideglusib is a glycogen synthase kinase-3 (GSK-3) inhibitor that belongs to the thiadiazolidine family.…”

“…19,20 The pulp capping materials should form a hermetic seal and promote wound healing. 1,4,9 Biocompatibility is also an important feature since these capping materials come into close touch with crucial pulp tissue for an extended period of time. 21 Cytotoxicity tests are routinely used to determine the biocompatibility of various materials when exposed to live cells.…”