Immunohistochemical Analysis of Endometrial Adenocarcinoma for bcl-2 and p53 in Relation to Expression of Sex Steroid Receptor and Proliferative Activity

Cell cycle regulators and apoptosis‐associated proteins in relation to proliferative activity and degree of apoptosis in HNPCC versus sporadic endometrial carcinoma

“…4,5 p53 overexpression has also previously been related to hormone receptor-negative tumours and poor survival. 31 Our results support the former relation. We found no direct correlation between the apoptosisregulating proteins and the apoptotic index, leading us to speculate on other apoptosis-regulating genes which play a larger role in apoptosis regulation in endometrial cancers.…”

“…3,[19][20][21][22][23][24][25][26][27][28][29][30][31] In the present study, the mean Mib-1 labelling index was 25%, which is slightly lower than that reported by others, 30-40%. 11,[31][32][33] The manner of selection of the control group could explain this slight discrepancy in Mib-1 labelling index. The Mib-1 labelling index in the sporadic endometrial cancers seemed to be predominantly regulated by cyclin E. Previously cyclin B1 and E have been reported to be involved in the regulation of proliferation in endometrial cancer.…”

p53 expression, p21 expression and the apoptotic index in endometrioid endometrial adenocarcinoma

“…radiotherapy. In this respect, it is interesting to note that p53 expression is an independent prognostic marker for poor survival in endometrial cancer 13,14,31,32 ; 5 year survival in patients with overexpression of nuclear p53 is 48%, compared to 80% for those in which there is no nuclear positivity. The implication is that aberrant p53 overexpression can occur by means other than point mutation and that it is p53 pathway dysfunction, and not p53 expression, that is a prognostic feature in these tumours.…”

“…8,9 Several studies have investigated p53 status immunohistochemically and have, in many cases, uncritically equated p53 protein expression with mutation of the tp53 gene. 10,11 Stewart et al 7 have shown that p53 protein expression may occur in endometrial carcinoma without mutation and may be due to stabilization of the protein during normal function.…”

Constitutive Tyrosine Phosphorylation of ErbB-2 via Jak2 by Autocrine Secretion of Prolactin in Human Breast Cancer

“…Results were expressed as percentage growth of untreated cells with random oligonucleotides alone. DNA synthesis was determined as described (40) Immunohistochemistry-Immunohistochemical analyses were performed as described (51). For immunostaining of PRL, PRLR, and ErbB-2, respective antibodies were diluted and incubated as follows: PRL (1:50, A569), PRLR (30 g/ml, U5) for 16 h at 4°C; ErbB-2 (1:200, A485) for 30 min at room temperature in a moisture chamber.…”