Immunohistochemical Analysis of Expression Patterns of Tumor Necrosis Factor Receptors on Lymphoma Cells in Enzootic Bovine Leukosis

Abstract

ABSTRACT. Tumor necrosis factor-alpha (TNF-α) has been reported to be associated with the progression of lymphoproliferative neoplastic diseases and retroviral infections. Hence we examined immunohistochemically the expression patterns of TNF-receptors (TNF-RI and RII) on lymphoma cells derived from the 29 cases of enzootic bovine leukosis (EBL). Lymphomas obtained in 29 animals with EBL were histopathologically classified into three types: diffuse mixed type (10 cases), diffuse large type (9 cases), and diffuse large cleaved type (10 cases). Immunohistochemically using a monoclonal antibody to a bovine lymphocyte surface antigen, the lymphomas were classified into three phenotypes: B-1a (CD5 + /CD11b + ), B-1b (CD5 -/CD11b + ) and B-2 (conventional B) (CD5 -/CD11b -). Interestingly, the lymphoma cells in all animals expressed TNF-RII, but not TNF-RI. Although, in EBL, lymphoma cells of which the histopathological and immunological property differs has been formed, the expression patterns of TNF-Rs had the universality in all lymphoma cells. TNF-RII, which induces cell proliferation, was expressed but TNF-RI, which induces cell apoptosis was not expressed on all lymphoma cells, suggesting that TNF-Rs play an important role in the malignant proliferation of B cells and formation of lymphomas in EBL. KEY WORDS: enzootic bovine leukosis, lymphoma, phenotype, tumor necrosis factor-α, tumor necrosis factor receptor.

Polymorphism and expression of the tumor necrosis factor receptor II gene in cows infected with the bovine leukemia virus

“…In cows with PL, more than 2-fold higher percentage of TNF-RII+ cells was noted in comparison to the non-infected animals. Previously, Ikeda et al (2005) detected the TNF-RII receptor only on neoplastic cells from cows with LS. The TNF-RII receptor was suggested to be the major factor responsible for the proliferative and anti-apoptotic effect of TNF-α (Tartaglia et al 1993, Aspalter et al 2003.…”

“…The TNF-RII epitope was identified using rabbit anti-human TNF-RII polyclonal IgG antibody (H-202) (working dilution 0.5 μg/50 μL) (Santa Cruz Biotechnology) and goat anti-rabbit IgG (H+L) secondary antibody conjugated to FITC (working dilution 1.0 μg/50 μL) (Invitrogen, USA). The specificity of H-202 antibody against bovine TNF-RII epitope was confirmed by Ikeda et al (2005). The same protocol was used for controls, but no primary antibodies were added.…”

Tumor necrosis factor-alpha genetic polymorphism may contribute to progression of bovine leukemia virus-infection

“…Twenty-five animals with lymphoma were diagnosed clinically, and confirmed by microscopic and histological examinations [15]. Other BLV-positive animals were classified as AL or PL based on the amount of leukocytes and the percentage of lymphocytes in their whole blood [13].…”

Polymorphisms in the bovine tumour necrosis factor receptor type two gene (TNF-RII) and cell subpopulations naturally infected with bovine leukaemia virus

“…Transactivator Tax protein encoded by the pX region of BLV and HTLV-1 genomes permanently activates the NF-κB signalling pathway, deregulates the expression of cellular genes (TNFα and others) and immortalises cells infected with HTLV-1 (15). It is also believed that TNFα and receptor TNF-RII play an important role in the progression of EBL in cattle (17). TNF/TNF-R signalling pathways participate in many cellular processes, including responses to viral infections (16).…”