Immunohistochemical analysis of factors related to apoptosis and cellular proliferation in relation to inflammation in dentigerous and odontogenic keratocyst

Sreedhar, Gadiputi; Raju, Menaka; Metta, Kiran Kumar; Manjunath, SM; Shetty, Sujan; Agarwal, Rakesh

doi:10.4103/0976-9668.127300

Cited by 10 publications

(10 citation statements)

References 8 publications

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“…Regarding Bcl-2 and Bax expressions, all cases evaluated showed some degree of immunoreactivity to these proteins. This finding was also observed by different studies that investigated the imbalance in cellular apoptosis in different tumors ( 15 - 17 ). We observed that Bcl-2 was similarly expressed in AOTs and AMBs, with a score of 2 (moderate) in most of the analyzed cases.…”

Section: Discussionsupporting

confidence: 80%

Apoptosis and cell cycle aberrations in epithelial odontogenic lesions: An evidence by the expression of p53, Bcl-2 and Bax

Tenório¹,

Santana²,

Queiroz³

et al. 2018

Med Oral

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BackgroundAmeloblastoma (AMB), odontogenic keratocyst (OKC) and adenomatoid odontogenic tumor (AOT) are epithelial odontogenic lesions with diverse biologic profiles. Defects in regulation of apoptosis and cell cycle may be involved in the development and progression of those lesions, therefore we aimed to investigate the expression of Bcl-2, Bax and p53 to better understand the possible role of these proteins in AMBs, OKCs and AOTs.Material and MethodsThe studied sample consisted of 20 AMBs, 20 OKCs and 20 AOTs. Immunohistochemistry technique was performed for the antibodies p53, Bcl-2 and Bax. Immunoreactivity was observed in the epithelial component and positive cells were counted in five fields (100x magnification). Statistical analysis was performed with Kruskal-Wallis and Spearman tests (p<0.05).ResultsAll lesions exhibited staining for the three studied proteins. There was no statistically significant associations between the expression of proteins and the lesions, however we identified a positive correlation between the expression of p53 and Bcl-2 (r = 0.200) and a negative correlation between p53 and Bax expressions (r = -0.100). In addition, p53 and Bax were similarly expressed between AMBs and OKCs. Bcl-2 was similarly expressed in AMBs and AOTs.ConclusionsApoptosis regulatory proteins, as well as cell cycle proteins, are differently expressed in epithelial odontogenic lesions and their expression is possibly related to the biological behavior of AMB, OKC and AOT. Key words:Odontogenic tumors, apoptosis, apoptosis regulatory proteins, p53 tumor suppressor protein, immunohistochemistry.

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Section: Discussionsupporting

confidence: 80%

Apoptosis and cell cycle aberrations in epithelial odontogenic lesions: An evidence by the expression of p53, Bcl-2 and Bax

Tenório¹,

Santana²,

Queiroz³

et al. 2018

Med Oral

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“…Four different tumors based on aggressiveness of their clinical behavior were studied in this study. The aggressive behavior of OKC was close to ameloblastoma as in many studies which investigated the expression of markers such as p53, p63, Ki67, PCNA, AgNors and Ipo (20)(21)(22)(23) and concluded that the proteins expressed on epithelial cells of OKC is more alike to that of ameloblastoma than DC.…”

Section: Discussionmentioning

confidence: 78%

Fascin expression in ameloblastoma, odontogenic keratocyst and dentigerous cyst

Rahrotaban

Nikparto²,

Yousefizadeh³

et al. 2018

TJPATH

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“…Particularly, they showed that while PCNA was markedly increased, Bcl-2 was significantly markedly in odontogenic keratocyst and dentigerous cyst. 18 In a different study comprising p53, Bcl-2 and Bax proteins, while expression of p53 and Bcl-2 was found to be positively correlated, expression of p53 and Bax protein was found to be negatively correlated. 19 In line with these findings, in tissues of odontogenic cysts, we also identified significant genetic alterations in genes significantly involved in the formation and progression of human cancers.…”

Section: Discussionmentioning

confidence: 93%

“…Also, both Ki-67 and p53 expressions were advanced in odontogenic keratocyst than dentigerous cyst, suggesting Ki-67 and p53 protein expression might be useful prognostic biomarker in odontogenic lesions. Sreedhar et al 18 also established the expression status of PCNA and BCL-2 proteins in odontogenic keratocyst and dentigerous cyst. Particularly, they showed that while PCNA was markedly increased, Bcl-2 was significantly markedly in odontogenic keratocyst and dentigerous cyst.…”

Section: Discussionmentioning

confidence: 99%

Deregulation of Cancer-Associated Genes in Odontogenic Cysts

Eğe

Yumrutaş

Bozgeyik

2019

Cumhuriyet Dental Journal

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The aim of the present study was to demonstrate the key role of differential expression levels of RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 genes in odontogenic cysts. Materials and Methods: A total number of 15 patients who diagnosed with odontogenic cyst were enrolled for the present study. For the quantitative gene expression analysis, cyst and adjacent gingival healthy tissues of patients were collected during surgical assessments. Quantitative analysis of gene expression levels RB1, TP53, XIAP, BCL2 AIFM3, BAX, CASP3 and CASP9 were achieved real-time PCR method. For the optimization of gene expression levels GAPDH reference gene was used. Results: Expression of both RB1 and TP53 genes were markedly diminished in odontogenic cysts tissues as compared to healthy tissues (p<0.05). Likewise, levels of CASP3 and CASP9 genes were found to be significantly reduced in odontogenic cysts tissues compared to healthy tissues (p<0.05). In contrast, expression levels of XIAP was significantly elevated (p<0.05). Although BCL2, AIFM3, and BAX genes were also differentially expressed in odontogenic cysts tissues, these variations were statistically insignificant (p>0.05). Conclusions: The findings of the present study indicates that RB1, TP53, XIAP, CASP3 and CASP9 genes might have chief roles in formation odontogenic cysts and responsible for the increased cell proliferation in these tissues.

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Immunohistochemical analysis of factors related to apoptosis and cellular proliferation in relation to inflammation in dentigerous and odontogenic keratocyst

Cited by 10 publications

References 8 publications

Apoptosis and cell cycle aberrations in epithelial odontogenic lesions: An evidence by the expression of p53, Bcl-2 and Bax

Apoptosis and cell cycle aberrations in epithelial odontogenic lesions: An evidence by the expression of p53, Bcl-2 and Bax

Fascin expression in ameloblastoma, odontogenic keratocyst and dentigerous cyst

Deregulation of Cancer-Associated Genes in Odontogenic Cysts

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