Immunohistochemical analysis of metastasising hepatocellular carcinomas in dogs

Abstract

ABSTRACT:In this study, the immunohistochemical features of primary hepatocellular carcinomas and their metastases in visceral organs, including the lungs, spleen and kidneys were examined using antibodies against carcino-embryonic antigen (CEA) cytokeratin (CK) 7 and 20, CD4, CD8, minichromosome maintenance protein 3 (MCM3), vimentin, and alpha 1 foetoprotein (AFP). In addition, Mallory's connective tissue stain, van Gieson's stain and Gomori methenamine silver stain were used. The study was performed on liver samples collected post mortem from five mixed-breed dogs aged 9-12 years. The tumours were classified according to the World Health Organization Classification of Tumours. Strong expression of MCM3 and AFP was found in the hepatic cancer cells and in the metastases to the lungs, spleen and kidneys. The primary tumours and metastatic foci did not react positively with the anti-CD4, anti-CD8, CEA, CK7 and CK20 antibodies. The connective tissue in the primary tumour and the metastases showed a positive reaction to vimentin. Canine hepatocellular carcinomas that metastasise are highly-malignant well-differentiated tumours that produce AFP and trace amounts of both carcinoembryonic antigen and cytokeratin. Therefore, the metastasis resembles the primary tumour and has a common phenotype and genotype with the primary tumour.

Acoustically Driven Microbubbles Enable Targeted Delivery of microRNA‐Loaded Nanoparticles to Spontaneous Hepatocellular Neoplasia in Canines

“…Likewise, in humans, it is well established that there is a ≈70% reduction in miR-122 expression in HCC as compared to that of normal tissue. [29] Since miR-122 expression level is consistently downregulated in HCC, it has been identified as a clinically relevant biomarker for staging and predicting the survival of human patients with HCC. [42] It controls neoplastic transformation by altering HOTAIR/miR-122/Cyclin G1 negative regulatory axis.…”

“…[28] In addition, the tumor development, phenotype and response to therapy in canines are similar to human HCC. [29,30] Thus, given the comparable anatomic size of humans and dogs afflicted with HCC, spontaneous liver tumors of canine may better mirror the therapeutic outcomes in patients with liver cancer. Moreover, mature miRNAs of human and dog possess high sequence homology when compared to many other species, which offers another advantage of replicating the same miRNAs for treatment of HCC in both dogs and humans.…”

“…[ 28 ] In addition, the tumor development, phenotype and response to therapy in canines are similar to human HCC. [ 29,30 ]…”

Exérese de carcinoma hepatocelular e leiomiossarcoma vaginal em uma cadela: Relato de caso

“…As neoplasias hepatobiliares em cães e gatos são incomuns, representando cerca de 2,6 e 5,5 % de todos os tumores nessas espécies, respectivamente (Ciaputa et al, 2016;Teshima et al, 2013;van Sprundel et al, 2014). Os tumores hepáticos dividem-se em quatro categorias: neoplasias hepatocelulares, ductos biliares, tumores neuroendócrinos e sarcomas.…”