Immunohistochemical analysis of periodontal reattachment on denuded root dentin after periodontal surgery

Abstract

This study evaluates the effects of three kinds of periodontal surgery using statistical analyses of histological and immunohistochemical indices. Dehiscence defects were made on roots of maxillary teeth in monkeys. Surgically exposed roots were untreated, etched with EDTA, or treated with Emdogain ® after EDTA etching. Paraffin sections were stained with hematoxylin and eosin or immunostained for bone sialoprotein (BSP) or osteopontin (OPN) and analysed using several indices. The relative length of regenerated cementum and of BSP/OPN-immunoreactive lines on dentin defect showed no differences among the three groups. The attached regenerated cementum in the untreated group was shorter than in the etching groups. Regenerated cementum area was larger in the etching-Emdogain group than in the etching group. Thickness of immunolabeling on detached cementum was larger than that on attached cementum in all of the groups. These findings suggest that etching reinforces the attachment of regenerated cementum, and that BSP and OPN are associated with the attachment, where they exercise strong adhesion within a certain level of thickness.

Pre‐existing root cementum may promote cementoblast differentiation of human periodontal ligament cells

“…These two proteins, which participate in initiating mineralization, are believed to play a major role in differentiation of cementoblast progenitor cells to cementoblasts (2). They are reported to be expressed in regenerated cementum, and also their expression is associated with new cementum attachment to dentine surfaces in a monkey model (25). OPN and BSP positive staining in our study indicated that newly formed matrix superficial to cementum was mineralization-related.…”

Acemannan sponges stimulate alveolar bone, cementum and periodontal ligament regeneration in a canine class II furcation defect model

“…Another possible cause of slit formation is tissue shrinkage during the paraffin sectioning process that can break the weak attachment between these two tissues . Recent studies have shown that bone sialoprotein and osteopontin play a role in adhesion between these two tissues .…”

Mechanical induction of interleukin‐11 regulates osteoblastic/cementoblastic differentiation of human periodontal ligament stem/progenitor cells

“…Bone sialoprotein is a significant component of mineralized tissues and is localized in cementum and bone . It has been reported that bone sialoprotein mediates the adhesion of cementum to dentin , and bone sialoprotein is considered to take part in cementogenesis along with osteopontin . In addition, bone sialoprotein has been suggested to contribute to PDL remodeling .…”