Immunohistochemical Analysis of Peritoneal Mesothelioma and Primary and Secondary Serous Carcinoma of the Peritoneum

Barnetson, Robert J.; Burnett, R A; Downie, Ian; Harper, Christina M.; Roberts, Fiona

doi:10.1309/8fchq3vpbwm7b5x9

Cited by 81 publications

(44 citation statements)

References 55 publications

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“…In contrast, ER and PR were reportedly negative in all malignant mesotheliomas of the peritoneum. 38,39 In this study, ER and PR were detected in 92 and 42% of papillary serous carcinomas of the ovary, respectively, similar to reported PAX2 in papillary serous carcinoma of the ovary G-X Tong et al findings. No ER or PR was detected in the 37 malignant mesotheliomas of the peritoneum in males that we studied.…”

Section: Discussionsupporting

confidence: 89%

Expression of PAX2 in papillary serous carcinoma of the ovary: immunohistochemical evidence of fallopian tube or secondary Müllerian system origin?

et al. 2007

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PAX2 is a urogenital developmental transcription factor expressed in the Wolffian ducts, developing kidneys, and Mü llerian ducts during embryonic stage. Its function in renal development is well documented and its clinical application in the diagnosis of lesions of renal origin has been reported recently. However, information on its role in the Mü llerian-derived genital tract is sparse. In this study, we investigated the expression of PAX2 in human female genital tract using immunohistochemistry. We demonstrated that PAX2 was expressed specifically in the epithelial cells of fallopian tube, endometrial and endocervical glands, but not in the stromal tissues in these areas. PAX2 was detected in secondary Mü llerian structures in the ovary, such as endometriotic and endosalpingiotic glands and rete ovarii, but not in ovarian surface epithelium, surface epithelium-derived inclusion cysts, stroma, or sex-cord-derived structures such as follicles, oocytes, and corpus luteum. In addition, PAX2 was detected in 67% of ovarian papillary serous carcinomas (N ¼ 36) but rarely in peritoneal malignant mesotheliomas, with two exceptions (N ¼ 54). Interestingly, the two PAX2-positive 'peritoneal malignant mesotheliomas' were from female patients and were positive for estrogen receptor. The significance of expression of PAX2 and estrogen receptor in these cases is under investigation. Taken together, we suggest that PAX2 is a novel Mü llerian-specific epithelial marker when used in proper clinical settings. Identification of PAX2 in the majority of papillary serous carcinomas of the ovary but not in the ovarian surface epithelium or epithelium-derived inclusion cysts suggests that this malignant epithelial tumor may be directly derived from the primary or secondary Mü llerian epithelium in or surrounding the ovary, rather than from the surface epithelium or its derivatives.

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Section: Discussionsupporting

confidence: 89%

Expression of PAX2 in papillary serous carcinoma of the ovary: immunohistochemical evidence of fallopian tube or secondary Müllerian system origin?

et al. 2007

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“…1 A number of comparative studies have been published in which the authors have attempted to determine the best panel of immunohistochemical markers that can assist in discriminating between peritoneal epithelioid mesotheliomas and serous carcinomas. [2][3][4][5][6][7][8][9][10] The recommended panels, however, have continually been subject to change as a result of the identification of new markers that can be used in the differential diagnosis of these tumors, as well as the publication of new information on the value of the individual markers. In a previous study by this author that was published in 2006 investigating the expression of a large number of positive mesothelioma markers (calretinin, podoplanin, keratin 5/6, and thrombomodulin) and positive carcinoma markers (MOC-31, Ber-EP4, BG-8 (Lewis y ), TAG-72 (B72.3), CD15 (leu-M1), and CA19-9), it was concluded that thrombomodulin, calretinin, and podoplanin were the best positive mesothelioma markers, and MOC-31 and Ber-EP4 were the best positive carcinoma markers for discriminating between peritoneal epithelioid mesotheliomas and serous carcinomas.…”

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confidence: 99%

Value of PAX8, PAX2, claudin-4, and h-caldesmon immunostaining in distinguishing peritoneal epithelioid mesotheliomas from serous carcinomas

Ordóñez

2013

Modern Pathology

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Distinguishing between peritoneal epithelioid mesotheliomas and papillary serous carcinomas involving the peritoneum can be difficult on routine histological preparations, but this differential diagnosis can be facilitated by the use of immunohistochemistry. Recent investigations have indicated that PAX8, PAX2, claudin-4, and h-caldesmon are immunohistochemical markers that can assist in distinguishing between these two malignancies; however, much of the information published on the value of these markers is either insufficient or contradictory. The purpose of this study is to resolve some of the existing controversies and to fully determine the practical value of these markers for assisting in the differential diagnosis between peritoneal mesotheliomas and serous carcinomas. In order to do so, a total of 40 peritoneal epithelioid mesotheliomas and 45 serous carcinomas (15 primary, 30 metastatic to the peritoneum) were investigated. PAX8 and PAX2 nuclear positivity was demonstrated in 42 (93%) and 25 (56%) of the serous carcinomas, respectively, whereas none of the mesotheliomas expressed either marker. Forty-four (98%) of the serous carcinomas exhibited claudin-4 reactivity along the cell membrane, whereas none of the mesotheliomas were positive for this marker. All of the serous carcinomas and mesotheliomas were negative for h-caldesmon. Based on these results, it is concluded that PAX8 and claudin-4 have a higher sensitivity and specificity for assisting in discriminating between peritoneal epithelioid mesotheliomas and serous carcinomas when compared with all of the other positive carcinoma markers that are, at present, recommended to be included in the immunohistochemical panels used in this differential diagnosis. Even though it is highly specific, PAX2 has little practical value in the diagnosis of peritoneal epithelioid mesotheliomas as its sensitivity is low. The h-caldesmon is not useful.

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“…Accordingly, the surface epithelium stains with vimentin, N-cadherin, calretinin, HBME1, CK7, and Ber-EP4 but it does not usually stain with CK20, and CA125 [1,3,9]. Besides, combinations of calretinin with Ber-EP4 as a marker of epithelial determinant which shows high expression in SC, and with ER that has an important role in the development of genital tract have been reported to be quite useful in the differential diagnosis of OSC with PMM [20][21][22][23]. In the study of Mozes, et al it has been reported that ER reactivity was demonstrated in 64.3% (36/56 cases) of OSC, 10.7% (4/37 cases) of ESC and 16.7% (1/6 cases) of TSC, whereas all PSC cases (5 cases) were negative [24].…”

Section: Discussionmentioning

confidence: 99%

“…In the study of Mozes, et al it has been reported that ER reactivity was demonstrated in 64.3% (36/56 cases) of OSC, 10.7% (4/37 cases) of ESC and 16.7% (1/6 cases) of TSC, whereas all PSC cases (5 cases) were negative [24]. In particular, since Ber-EP4 is expressed in SCs in high levels, it is an important marker used in the differentiation of SC and PMM [20][21][22]25]. High rate of ER positivity is observed in OSC.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of the Relationship between Malignant Tumors Originated from Peritoneal and Müllerian Epithelium with the Primary and Secondary Müllerian Systems-Immunohistochemical Analysis with Six Markers

Çiftçi¹

2017

Int J Pathol Clin Res

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The origin of ovarian epithelial tumors is still indistinct. Mülle-rian and coelomic hypothesis are controversial. Recently, there is also a small number of reports that tubo-peritoneal junction may play an important role in the development of tubo-ovarian tumors. Therefore, we aimed to investigate the relationship between tumors originating from Mullerian epithelium and peritoneal mesothelium, in other words primary and secondary Müllerian systems.Seventy malignant tumors originating from the Müllerian epithelium and peritoneal mesothelium were included in the study. Tumors were analysed immunohistochemically for PAX8, WT1, CD56, Ber-EP4, ER and desmin expression. We evaluated the study based on three phases. Firstly, we investigated immunohistochemical stainings of different tumor types in different locations. Secondly, we assessed immunohistochemical characteristics according to their histologicals subtypes, and finally their relationship with primary or secondary Müllerian systems.As a result different immunohistochemical features were detected between both localization and histological types of these tumor groups.Absence of immunoreactivity of PAX8, WT1 and CD56 in endometrial serous carcinomas and, the presence of high immunoreactivity in ovarian and tubal serous carcinomas was considered to be an indicator of different origins of these tumors as primary and secondary Müllerian systems. It also seems possible that tubo-peritoneal junction could be the junction between primary and secondary Müllerian systems and, may play an important role in the development of tubo-ovarian tumors.

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Immunohistochemical Analysis of Peritoneal Mesothelioma and Primary and Secondary Serous Carcinoma of the Peritoneum

Cited by 81 publications

References 55 publications

Expression of PAX2 in papillary serous carcinoma of the ovary: immunohistochemical evidence of fallopian tube or secondary Müllerian system origin?

Expression of PAX2 in papillary serous carcinoma of the ovary: immunohistochemical evidence of fallopian tube or secondary Müllerian system origin?

Value of PAX8, PAX2, claudin-4, and h-caldesmon immunostaining in distinguishing peritoneal epithelioid mesotheliomas from serous carcinomas

Investigation of the Relationship between Malignant Tumors Originated from Peritoneal and Müllerian Epithelium with the Primary and Secondary Müllerian Systems-Immunohistochemical Analysis with Six Markers

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