Immunohistochemical Analysis of S100 Proteins in Normal and Irreversibly Inflamed Human Dental Pulps

Jungbluth, Holger; Kaiser, Meta Lena Britta; Lalaouni, Diana; Winter, Jochen; Jepsen, Søren

doi:10.1016/j.joen.2023.02.003

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…A number of studies have investigated inflammation of the dental pulp (7)(8)(9)(10); however, further clarification of the mechanism of pulp inflammation is expected to lead to more reliable treatment of pulpitis. Suppression of pulpal inflammation and induction of remineralization have also been investigated (7,(11)(12)(13) and are considered to be important in dental treatment to preserve the dental pulp or to regenerate the tooth structure (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Chemokine‑like receptor 1‑positive cells are present in the odontoblast layer in tooth tissue in rats and humans

Yokokawa,

Umemura,

Miyamoto

et al. 2023

Exp Ther Med

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Cluster of differentiation (CD)44 is a marker of dental pulp stem cells and is involved in odontoblast differentiation and calcification. Chemokine-like receptor 1 (CMKLR1), also known as chemerin receptor 23 (ChemR23) is also expressed in odontoblasts and dental pulp stem cells and is involved in inflammation suppression and tooth regeneration. Resolvin E1, a bioactive lipid, is a CMKLR1 ligand that mediates the chemerin-CMKLR1 interaction and suppresses pulpal inflammation. The present study clarified the intracellular and tissue localization of CD44 and CMKLR1 by immunohistochemical staining of normal pulp and pulp with pulpitis from 12-week-old male Wistar rat teeth or human teeth. In addition, the localization of CD44 and CMKLR1 in human dental pulp stem cells was observed by immunofluorescence staining. The present study also examined the involvement of resolvin E1 in inhibiting inflammation and calcification by western blotting. CD44- and CMKLR1-positive cells were confirmed in the odontoblast layer in normal dental pulp of rats and humans. CD44 was mainly localized in the cell membrane and CMKLR1 was mainly found in the cytoplasm of human dental pulp stem cells. CMKLR1 was also confirmed in the odontoblast layer in rats and humans with pulpitis but CD44 was not present. Following treatment of dental pulp stem cells with lipoteichoic acid, which imitates Gram-positive bacterial infection, resolvin E1 did not suppress the expression of cyclooxygenase-2 or of the odontoblast differentiation marker, dentin sialophosphoprotein. Furthermore, resolvin E1 induced the differentiation of dental pulp stem cells into odontoblasts even in the presence of the inflammatory stimulus.

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