Immunohistochemical analysis of unsutured and sutured corneal wound healing

Abstract

In the unsutured partial thickness penetrating wounds of the regions. In sutured wounds, effective wound repair may be cornea, the epithelium migrates over the wounded stromal established from the anterior to posterior regions, after a surface prior to the onset of stromal regeneration. To determine relatively long period of a disorganized state of the scar tissues, the possible affects of the epithelial ingrowth on the organiza tion of the stromal scar tissues, the healing of unsutured and sutured wounds was compared immunohistochemically. Immunostaining patterns for fibronectin, types III, VI and VII These observations suggest that the temporary presence of an epithelial plug within an unsutured wound and/or wound gaping induce a different stromal healing response from that in the sutured wounds, that results in an eventual ineffective collagen, keratan sulfate proteoglycan (KSPG), and interwound repair. Curr. Eye Res. 14: 809-817, 1995. mediate filament-associated protein (IFAP 130) in fibroblasts, were analyzed in unsutured and adjacent sutured keratotomy w ounds in monkeys, at 2-9 weeks after surgery. At 2-4 weeks, fibronectin, type III and type VI collagen showed a lamellar interweaving pattern across unsutured wounds that was absent in sutured wounds. Type VII collagen was detected along the entire depth of regenerated stroma in unsutured wounds, but not in sutured wounds indicating that the epithelium had

In vivo architectural analysis of 3.2 mm clear corneal incisions for phacoemulsification using optical coherence tomography

“…However, persistence of these irregularities might be related not only to stromal edema as a result of significant endothelial cell damage from direct mechanical trauma, ultrasound energy, and irrigating solution during the surgery but also to a more disorganized wound-healing response in the posterior stroma. [25][26][27][28][29][30] In contrast to in vitro studies, 9,10 gaping did not translate to the epithelial margin of the wound in our study. The adequate sealing after single-plane incisions that we found may be explained by multiple factors.…”

Extracellular Matrix Changes in Human Corneas After Radial Keratotomy

“…The staining for epithelial BM components becomes discontinuous. The abnormal ECM deposition in the stroma continues for about a month, and then the deposits begin to disappear (SundarRaj et al, 1990 ;Kahle et al, 1991 ;Rowe et al, 1992 ;van Setten et al, 1992 ;Latvala et al, 1995a ;Melles et al, 1995b ;Anderson et al, 1996). By 12 months post refractive surgery, normal ECM and BM pattern is usually restored (SundarRaj et al, 1990 ;Balestrazzi et al, 1995 ;Latvala et al, 1995a ;Anderson et al, 1996).…”

“…First, a provisional ECM is formed and it is then gradually replaced by permanent ECM of a healed scar (Garana et al, 1992 ;Jester et al, 1992 ;Stock et al, 1992 ;Assil and Quantock, 1993 ;Melles et al, 1995b). ECM and BM components including fibronectin, tenascin-C, laminin, types III, IV, VII collagen, hyaluronan and stromal proteoglycans, undergo transient changes in distribution during wound healing.…”

“…This process involves an extensive remodeling of corneal extracellular matrix (ECM) and epithelial basement membrane (BM) that is apparently due to the activity of myofibroblasts. Transient changes of several ECM components and of β % integrin (epithelial adhesion complex component) during corneal wound healing have been documented (Malley et al, 1990 ;SundarRaj et al, 1990 ;Kahle et al, 1991 Balestrazzi et al, 1995 ;Latvala et al, 1995a ;Latvala, Tervo and Tervo, 1995b ;Melles et al, 1995b ;Anderson et al, 1996), mostly after photorefractive keratectomy (PRK). This procedure involves mechanical removal of corneal epithelium, and shaving off Bowman's layer and anterior part of the corneal stroma with an ultraviolet 193 nm excimer laser.…”

Proteoglycan Distribution During Healing of Corneal Stromal Wounds in Chick

“…The composition and organization of the stromal matrix are altered in scar tissues that are formed following penetrating wounds (Cintron et al, 1978 ;Goodman et al, 1989 ;Tervo et al, 1989 ;Malley et al, 1990 ;SunderRaj et al, 1990 ;Tervo et al, 1991 ;Melles et al, 1995). Collagens-types I, V, VI and XII (Newsome, Gross and Hassell, 1982 ;Birk, Fitch and Linsenmayer, 1986 ;Linsenmayer et al, 1986 ;Zimmermann et al, 1986 ;Alper, 1988 ;Birk et al, 1990 ;Gordon et al, 1996 ;Linsenmayer et al, 1990 ;Wessel et al, 1997), and proteoglycans containing keratan sulfate and dermatan sulfate (Dische, CremerBartels and Kaye, 1985 ;Scott and Haigh, 1985 ;Funderburgh, Caterson and Conrad, 1986 ;Takahashi et al, 1993 ;Zhan, Burrows and Cintron, 1995 ;Corpuz et al, 1996 ;Funderburgh et al, 1997) are the major components of the corneal stromal matrix.…”