Immunohistochemical analysis of vascular endothelial growth factor cellular expression in ovarian endometriomata

Goteri, Gaia; Lucarini, Guendalina; Filosa, Alessandra; Pierantoni, Alessandra; Montik, Nina; Biagini, G.; Fabris, Guidalberto; Ciavattini, Andrea

doi:10.1016/j.fertnstert.2003.11.035

Cited by 37 publications

(26 citation statements)

References 23 publications

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“…Many studies have confirmed that EMS and angiogenesis are closely related (4)(5)(6)(7)(8)21). Microvessel density (MVD) is a quantitative description of angiogenesis.…”

Section: ------------------------------------------------------------mentioning

confidence: 97%

“…When shed and attached to the peritoneal surface or other body parts, the ectopic endometrial tissue rebuilds itself and the surrounding glands and stroma. Endometrial lesions grow quickly and this growth requires many new blood vessels; therefore, blood vessel formation promotes survival of ectopic endometrial implantation in basic conditions and in EMS (3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory effect of curcumin on angiogenesis in ectopic endometrium of rats with experimental endometriosis

Zhang¹

2010

Int J Mol Med

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Abstract. The aim of this study was to observe the inhibitory effect of curcumin on endometriosis (EMS) and to determine its influence on vascular endothelial growth factor (VEGF) and microvessel density (MVD) in eutopic and ectopic endometrium of experimental rats, thus exploring the pathogenesis of EMS offering more experimental evidence for the clinical use of curcumin. Forty-eight female virgin rats were subjected to autotransplantation of endometrium during the estrus stage. After four weeks, 8 rats were randomly sacrificed to confirm that the rat model was successful. The remaining rats were randomly divided into four groups. Three groups were intragastrically administered curcumin (50, 100 and 150 mg/kg), and the model group was intragastrically administered vehicle alone. All rats were treated daily for four continuous weeks and examined by histology and immunohistochemical staining for MVD of eutopic and ectopic endometrium. Our results revealed that the cubic capacity of focal tissue in gross appearance was high in the model group and dose-dependently diminished after treatment with curcumin (P<0.05). There was an increase in MVD and VEGF in the ectopic endometrium, which was decreased significantly after treatment with curcumin (P<0.05); the effects being dose-dependent. The correlation between MVD and VEGF was positive. In conclusion, heterogeneity was found to exist between eutopic and ectopic endometrium due to differences noted in MVD and the expression of VEGF between the eutopic and ectopic endometrium in the model group. Curcumin decreased the quantity of microvessels and VEGF protein expression in the heterotopic endometrium of rats with EMS.

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“…Many studies have confirmed that EMS and angiogenesis are closely related (4)(5)(6)(7)(8)21). Microvessel density (MVD) is a quantitative description of angiogenesis.…”

Section: ------------------------------------------------------------mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of curcumin on angiogenesis in ectopic endometrium of rats with experimental endometriosis

Zhang¹

2010

Int J Mol Med

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“…Previous studies have supported the crucial role of vascular endothelial growth factor (VEGF) in the angiogenesis occurring in endometriosis: this molecule is involved in the normal turnover of endometrium and during pregnancy; its role is to increase the permeability of endometrial blood vessels and stimulate proliferation and migration of endothelial cells [11][12][13]. In ovarian endometriotic cysts, VEGF is expressed in the ectopic endometrial glandular epithelium and stromal cells; either a negative or positive correlation of VEGF expression has been reported with cyst diameter [10,[14][15][16][17][18]. VEGF production is regulated by various stimuli, including steroid hormones, hypoxia and nitric oxide (NO) levels [19].…”

Section: Introductionmentioning

confidence: 94%

Proangiogenetic molecules, hypoxia-inducible factor-1α and nitric oxide synthase isoforms in ovarian endometriotic cysts

et al. 2010

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Endometriosis is a common disease characterised by ectopic growth of endometrial tissue outside the uterine cavity. Angiogenesis has been implicated in the pathogenesis of the disease; some molecules, like hypoxia-inducible factor-1alpha (HIF-1alpha) and neuronal, endothelial and inducible nitric oxide synthase isoforms (nNOS, eNOS and iNOS), are known as proangiogenetic factors. We evaluated expression of these molecules by immunohistochemistry in 32 cases of ovarian endometriomas, formalin-fixed and paraffin-embedded. Analysis was focused on the cells composing the inner layer of the cyst, constituted by the ectopic endometrial glands, stromal cells and vessels, and the outer one, constituted by a fibrous layer of fibroblasts and vessels. We found that epithelial glands and capsular vessel endothelial cells showed a correlated expression of NOS isoforms; that expression of nNOS, iNOS and HIF-1alpha was correlated in epithelial glands and capsular fibroblasts; that vessel endothelial cells showed a higher mean expression for all the proangiogenetic molecules in the outer layer than in the inner one; and that capsular fibroblasts showed a higher mean expression for HIF-1alpha, iNOS and eNOS compared to the specialised stromal cells of the inner layer. Our data seem to indicate that angiogenesis is stimulated more in the outer capsule than in the inner layer of ovarian endometriotic cysts. The knowledge of the complex mechanisms associated to angiogenesis might be useful in a therapeutic approach of ovarian endometriosis based on anti-angiogenetic drugs. The therapeutic target would be mainly the capsular vasculature, more than the vasculature of ectopic endometrial tissues.

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“…( Goteri G. et al, 2004). The inner layers of cysts are characterized by high microvessel density but low expression of VEGF, whereas in the outer fibrosclerotic capsule, the vessels were less abundant, but Had a higher expression of VEGF and survive, thus activated to proliferate and protected from programmed cells death.…”

Section: Vegf Role In Endometriosismentioning

confidence: 99%

Endometriosis and Angiogenic Factors

Artini¹,

Ruggiero²,

Papini³

et al. 2012

Endometriosis - Basic Concepts and Current Research Trends

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Immunohistochemical analysis of vascular endothelial growth factor cellular expression in ovarian endometriomata

Cited by 37 publications

References 23 publications

Inhibitory effect of curcumin on angiogenesis in ectopic endometrium of rats with experimental endometriosis

Inhibitory effect of curcumin on angiogenesis in ectopic endometrium of rats with experimental endometriosis

Proangiogenetic molecules, hypoxia-inducible factor-1α and nitric oxide synthase isoforms in ovarian endometriotic cysts

Endometriosis and Angiogenic Factors

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