Immunohistochemical and developmental studies to elucidate the mechanism of action of the snowdrop lectin on the rice brown planthopper, Nilaparvata lugens (Stal).

Powell, Kevin; Spence, Jaqui; Bharathi, M.; Gatehouse, John A.; Gatehouse, A. M. R.

doi:10.1016/s0022-1910(98)00054-7

Cited by 172 publications

(113 citation statements)

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“…Three modes of action of these dietary lectins have been identified: (1) binding of the lectins to the peritrophic matrix of the midgut and disrupting nutrient absorption (Harper et al, 1998); (2) binding to glycoproteins on epithelial cells of the midgut and disrupting tissue integrity (Powell et al, 1998;Sauvion et al, 2004); and (3) binding of lectins to carbohydrate moieties of the sensory receptors of the insect mouth parts, disrupting membrane integrity and interfering in the ability of the insects to detect food (Murdock and Shade, 2002). All three of these mechanisms result in decreased ability of the insects to ingest or absorb nutrients, leading to delayed development and premature death.…”

Section: Discussionmentioning

confidence: 99%

“…The snowdrop lectin, GNA, is toxic to brown planthopper (Nilaparvata lugens; Powell et al, 1998;Tang et al, 2001) and tomato moth (Lacanobia oleracea) larvae (Fitches et al, 1997;Gatehouse et al, 1997Gatehouse et al, , 1999 as well as to several aphid species (Hilder et al, 1995;Rahbé et al, 1995;Gatehouse et al, 1996;Sauvion et al, 1996). Concanavalin A (lectin from Canavalia ensiformis) shows significant antimetabolic activity toward third-instar nymphs of taro planthopper (Tarophagous proserpina; Powell, 2001).…”

mentioning

confidence: 99%

“…The insecticidal activity is attributed to lectin recognition of Man glycans in the digestive tracts of insects (Fitches et al, 2001;Majumder et al, 2004). Immunohistochemical studies show that these lectins bind to carbohydrate moieties of glycoproteins on epithelial cells lining the luminal surface of insect midguts, such as the binding of GNA in brown planthopper (Powell et al, 1993(Powell et al, , 1998 and concanavalin A in pea aphids (Acyrthosiphon pisum; Sauvion et al, 2004). An additional target is the peritrophic matrix lining the midgut region.…”

mentioning

confidence: 99%

“…Concanavalin A and GNA bind to many peritrophic matrix proteins in vitro (Fitches and Gatehouse, 1998). The binding of lectins to these tissues results in disruption of the digestive processes and nutrient assimilation (Eisemann et al, 1994) by damaging the structure of the insect brushborder epithelium (Harper et al, 1995) and altering the organization of the microvilli (Powell et al, 1998). Consequently, the insect may be repelled, retarded in its growth, or even killed.…”

mentioning

confidence: 99%

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Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

et al. 2008

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We previously cloned and characterized a novel jacalin-like lectin gene from wheat (Triticum aestivum) plants that responds to infestation by Hessian fly (Mayetiola destructor) larvae, a major dipteran pest of this crop. The infested resistant plants accumulated higher levels of Hfr-1 (for Hessian fly-responsive gene 1) transcripts compared with uninfested or susceptible plants. Here, we characterize the soluble and active recombinant His 6 -HFR1 protein isolated from Escherichia coli. Functional characterization of the protein using hemagglutination assays revealed lectin activity. Glycan microarray-binding assays indicated strong affinity of His 6 -HFR1 to Mana1-6(Mana1-3)Man trisaccharide structures. Resistant wheat plants accumulated high levels of HFR1 at the larval feeding sites, as revealed by immunodetection, but the avirulent larvae were deterred from feeding and consumed only small amounts of the lectin. Behavioral studies revealed that avirulent Hessian fly larvae on resistant plants exhibited prolonged searching and writhing behaviors as they unsuccessfully attempted to establish feeding sites. During His 6 -HFR1 feeding bioassays, Drosophila melanogaster larvae experienced significant delays in growth and pupation, while percentage mortality increased with progressively higher concentrations of His 6 -HFR1 in the diet. Thus, HFR1 is an antinutrient to dipteran larvae and may play a significant role in deterring Hessian fly larvae from feeding on resistant wheat plants.

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confidence: 99%

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Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

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“…GNA was also detected in fat bodies, ovarioles, and hemolymph (Powell et al, 1998). Ovarioles especially are known as a sink for circulating molecules.…”

Section: Plant Lectins Principally Bind To Receptors In the Insect Gutmentioning

confidence: 94%

Plant‐insect interactions: what can we learn from plant lectins?

Michiels

Damme

Smagghe

2010

Arch Insect Biochem Physiol

120

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Many plant lectins have high anti-insect potential. Although the effects of most lectins are only moderately influencing development or population growth of the insect, some lectins have strong insecticidal properties. In addition, some studies report a deterrent activity towards feeding and oviposition behavior. Transmission of plant lectins to the next trophic level has been investigated for several tritrophic interactions. Effects of lectins with different sugar specificities can vary substantially with the insect species under investigation and with the experimental setup. Lectin binding in the insect is an essential step in exerting a toxic effect. Attempts have been made to study the interactions of lectins in several insect tissues and to identify lectin-binding receptors. Ingested lectins generally bind to parts of the insect gut. Furthermore, some lectins such as the Galanthus nivalus agglutinin (GNA) cross the gut epithelium into the hemolymph and other tissues. Recently, several candidate lectinbinding receptors have been isolated from midgut extracts. To date little is known about the exact mechanism for insecticidal activity of plant lectins. However, insect glycobiology is an emerging research field and the recent Grant sponsor: Fund for Scientific Research-Flanders, Brussels; Grant number: 3G016306.Correspondence to: Guy Smagghe, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. E-mail: guy.smagghe@ugent.be Vol. 73, No. 4, 193-212 (2010 ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,

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Engineering Resistance to Insect Pests

Ferry

Edwards

Mulligan

et al. 2004

Handbook of Plant Biotechnology

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With a projected increase in world population to 10 billion over the next four decades, an immediate priority for agriculture is to achieve maximum production of food and other products in a manner which is environmentally sustainable and cost effective. Despite the synthesis of improved pesticides, and integrated pest management strategies, yield losses due to insects have actually increased slightly for most crops over the last two decades. The concept of utilising a transgenic approach to host plant resistance was realised in the mid 1990s with the commercial introduction of genetically modified crops expressing genes encoding the entomocidal d‐endotoxin from Bacillus thuringiensis ( Bt ). More recently this strategy has been extended to include the pyramiding (stacking) of genes encoding different Bt toxins for greater levels of pest control. Although not as yet a commercial reality, other strategies based on the use of plant derived genes (enzyme inhibitors, lectins) and those from animal sources, including insects (biotin‐binding proteins, neurohormones, enzyme inhibitors), are being developed. The use of fusion proteins to increase the spectrum and durability of resistance is also actively being pursued. However, if transgenic insect‐resistant crops are to play a useful role in crop protection, it is apparent that they must be compatible with the other components of integrated pest management (IPM). The current chapter addresses the role of insect‐resistant transgenic crops in agriculture and discusses both their current status and future developments. Given the importance of natural enemies in controlling pest populations, the potential impact of such crops on predators and parasitoids is also addressed.

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Immunohistochemical and developmental studies to elucidate the mechanism of action of the snowdrop lectin on the rice brown planthopper, Nilaparvata lugens (Stal).

Cited by 172 publications

References 28 publications

Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

Functional Characterization of HFR1, a High-MannoseN-Glycan-Specific Wheat Lectin Induced by Hessian Fly Larvae

Plant‐insect interactions: what can we learn from plant lectins?

Engineering Resistance to Insect Pests

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