Immunohistochemical and Enzyme Histochemical Studies in Bone Formation Induced by Bone Morphogenetic Protein (BMP) in Mouse Muscle Tissue

Kamegai, Akihide; Tanabe, Toshiichiro; Shimamura, Norimasa; Kumasa, Shunsuke; Yamada, Kazuto; Mori, Masahiko

doi:10.1007/978-4-431-68132-8_19

Cited by 5 publications

(4 citation statements)

References 15 publications

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“…Fibronectin has been localized in demineralized bone matrix when implanted in vivo, mesenchymal cells before and during cell proliferation, newly synthesized extracellular matrix, differentiating chondrocytes, and in cottony array around osteoblasts [9,11,19]. Changes in the expression and functional role of fibronectin have been reported during myogenesis, muscle regeneration, and differentiation of cartilage and bone [13,14]. Laminin is important component of all basement membranes, and it plays a role in determining the architecture and integrity of various tissues [15].…”

Section: Introductionmentioning

confidence: 99%

Laminin and fibronectin in bone formation induced by bone morphogenetic protein (BMP) in mouse muscle tissue

1994

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Abstract:The morphological features at the light and electron microscopic level and distribution of fibronectin and laminin using immunohistochemical methods were evaluated in chondro-osteogneic tissue induced by bone morphogenetic protein (BMP) in mouse skeletal muscle. Satellite cells were seen at the muscle fibers adjacent to the site of BMP implantation 3 days after BMP-implantation. At the ultrastructuraI level, the skeletal muscle had a continuous basal lamina, satellite cells were partially enveloped by basal lamina-like structure, and fibroblast-like cells with abundant mitochondria and endoplasmic reticulum were adjacent to the myofibrils. An enhanced immunoreactivity for laminin and fibronectin was observed at the basement membrane of muscle fibers and extracellular ~natrix of neighboring connective tissue on day 3. On the 7th day, the enhanced immunoreactivity of laminin was persistent on the muscle fibers adjacent to the BMP and myoblast-like satellite cells, however, fibronectin showed decreased reaction. On the 14th day specimen, the newly formed osteo-chondroid matrix with numerous chondroid cells and osteoblasts showed positive immunoreaction for laminin and fibronectin. During the process of resorption of the induced osteoid tissue, no immunoreactivity for laminin and fibronectin was observed. An enhanced immunoreactivity of these glycoproteins during the early stages of BMP induced chondro-ossification suggests that they play an important role in the differentiation of myo-fibroblasts and organization and integration of ground substances of connective tissue and chondro-osseous matrix.

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Section: Introductionmentioning

confidence: 99%

Laminin and fibronectin in bone formation induced by bone morphogenetic protein (BMP) in mouse muscle tissue

1994

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“…Numerous reports have been made, indicating BMP induces heterotopic bone formation via chondrogenesis in mouse skeletal muscle tissues (14,18,21,27,31,33,34). Perivascular mesenchymal cells (31,33), muscle sheath cells, and myoblasts (18,21,27,34) have been suggested as progenitor cells.…”

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confidence: 99%

“…Perivascular mesenchymal cells (31,33), muscle sheath cells, and myoblasts (18,21,27,34) have been suggested as progenitor cells. Histologically, the process of BMP-induced heterotopic bone formation has been recorded (14,18,33). Tissues implanted with BMP showed cell infiltrations from the mesenchyme into the implanted areas and the rapid differentiation of these cells into chondroidal cells.…”

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confidence: 99%

“…GAGs have been reported to be greatly concerned with ossification cascade (14,20,22,24). In view of these, distribution and localization of GAGs during the course of BMP induced chondro-osteogenesis are supposed to have great concern with cells migration, differentiation, and transformation, thus suggesting matrix macromolecules coordinately regulate bone formation.…”

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confidence: 99%

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Glycosaminoglycan Distribution in Chondro-Osteogenesis Induced by Bone Morphogenetic Protein in Mouse Muscle Tissue with the Use of Monoclonal Antibodies.

Nakamine

Kamegai

Yasuda

et al. 1994

Acta Histochem. Cytochem

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Osteoinduktion und- reparation

Kübler

1997

Mund Kiefer GesichtsChir

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Traumata, diseases, developmental deformities, and tumor resections frequently cause bone defects and atrophies. In general, three different mechanisms exist by which bone restoration can be achieved: (1) osteogenesis initiated by vital, osteoblastic cells of autografts; (2) osteoconduction (or creeping substitution); and (3) osteoinduction. The latter mechanism means the differentiation of pluripotent, mesenchymal-type cells (located in a recipient bed with strong regenerative capacity) into cartilage- and bone-forming progenitor cells under the influence of inductive bone morphogenetic proteins (BMPs). Some BMPs are physiologically included in low concentrations as organic components in bone tissue. They can diffuse from demineralized bone implants into the recipient bed and induce a differentiation into new bone tissue. Nine different BMPs have been isolated, characterized, and cloned. Some of these possess inductive properties and can initiate new bone formation in muscle tissue or in bone defects. In the future recombinant BMPs will be available in unlimited quantities. This will lead to completely new therapeutic concepts in reconstructive bone surgery.

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Immunohistochemical and Enzyme Histochemical Studies in Bone Formation Induced by Bone Morphogenetic Protein (BMP) in Mouse Muscle Tissue

Cited by 5 publications

References 15 publications

Laminin and fibronectin in bone formation induced by bone morphogenetic protein (BMP) in mouse muscle tissue

Laminin and fibronectin in bone formation induced by bone morphogenetic protein (BMP) in mouse muscle tissue

Glycosaminoglycan Distribution in Chondro-Osteogenesis Induced by Bone Morphogenetic Protein in Mouse Muscle Tissue with the Use of Monoclonal Antibodies.

Osteoinduktion und- reparation

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