Immunohistochemical and structural characteristics of the reticular framework of the white pulp and marginal zone in the human spleen

Satoh, Takashi; Takeda, Rikio; Oikawa, Hiroki; Satodate, Ryoichi

doi:10.1002/(sici)1097-0185(199712)249:4<486::aid-ar8>3.0.co;2-p

Cited by 31 publications

(31 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At ultrastructural examination, reticulum cells display bundles of microfilaments (Pinkus et al 1986;Satoh et al 1997). A subpopulation of reticulum cells expresses the smoothmuscle isoform of actin (a-SMA).…”

Section: Discussionmentioning

confidence: 99%

“…In human and rat spleen, a-SMAcontaining reticular cells were also demonstrated in the PALS; but in follicles, these cells were rare, corresponding to sparse reticulum cells and reticulin fibers in this area. FDCs may replace a-SMA-positive reticular cells in germinal centers (Toccanier-Pelte et al 1987;Satoh et al 1997Satoh et al ,2009Steiniger et al 2001). In vivo imaging has shown that the FDC network displays a marked mobility, and FDCs in culture express a-SMA (Bajénoff et al 2006;Muñoz-Fernández et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…The reticular network must permit great volume changes during an immune response, e.g., associated with the development and regression of germinal centers (Veerman and Vries 1976;Liu et al 1991;Hollowood and Macartney 1992). Accordingly, contractile proteins characteristic of smooth muscle are expressed by reticular cells (Pinkus et al 1986;Toccanier-Pelte et al 1987;Satoh et al 1997Satoh et al ,2009Steiniger et al 2001).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

Köhler

2009

J Histochem Cytochem.

View full text Add to dashboard Cite

Reticular cells and follicular dendritic cells (FDCs) build up a framework that underlies the compartmentalization of spleens and lymph nodes. Subpopulations of reticular cells express the smooth-muscle isoform of actin, indicative of a specialized contractile apparatus. We have investigated the distribution of the actin-binding protein caldesmon in spleen and lymph nodes of mice and rats. Caldesmon modulates contraction and regulates cell motility. Alternative splicing of transcripts from a single gene results in high-molecular-mass isoforms ( h-caldesmon) that are predominately expressed by smooth-muscle cells (SMCs), and low-molecular-mass isoforms ( I-caldesmon) that are thought to be widely distributed in non-muscle tissues, but the distribution of caldesmon in spleen and lymph nodes has not been reported. We have performed Western blot analysis and immunohistochemistry using four different antibodies against caldesmon, among these a newly developed polyclonal antibody directed against recombinant mouse caldesmon. Western blot analysis showed the preponderance of I-caldesmon in spleen and lymph nodes. Our results from immunohistochemistry demonstrate caldesmon in SMCs, as expected, but also in reticular cells and FDCs, and suggest that the isoform highly expressed by reticular cells is I-caldesmon. In spleen of SCID mice, caldesmon was expressed by reticular cells in the absence of lymphocytes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

Köhler

2009

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…Lymph follicles predominate in the human spleen whereas the PALS predominates in the rat (Steiniger et al 1997). The reticular framework of the white pulp is divided into 2 parts in mice (Tanaka et al 1996) and man (Satoh, 1997), one part surrounding the PALS and the other surrounding the lymph follicles. The marginal zone is the site of heavy blood traffic and filtration.…”

Section: mentioning

confidence: 99%

The spleen of the one humped camel (Camelus dromedarius) has a unique histological structure

et al. 2000

View full text Add to dashboard Cite

The histology and structure of 38 spleens of the dromedary (aged 0n5-15 y) were studied in relation to age. The spleen was found to have a thick capsule (292p106 mm) divided into an outer layer (113p39 mm) composed mainly of connective tissue and an inner layer (180p81 mm) consisting mainly of smooth muscle cells. Vascular and avascular trabeculae extend from the capsule, the former containing arteries and nerves but no trabecular veins, the latter being divided structurally into primary and secondary trabeculae. Subcapsular and peritrabecular blood sinuses around primary and vascular trabeculae are unique to the camel spleen. The central artery emerges from the periarterial lymphatic sheath and branches into up to 4 penicilli which extend as sheathed arterioles (42p8 µm). These are found near or surrounded by blood sinusoids of the red pulp. A wide marginal zone surrounds the white pulp and contains sheathed arteries but no marginal sinuses. The red pulp is characteristically divided into cords by secondary trabeculae and contains venous sinusoids of different sizes. The camel spleen is of a sinusal type that can store blood. The thick muscular capsule and trabeculae pump the stored blood according to the body's need. Both closed and open circulations are found. The venous return is unique as the blood flow is from the venous sinusoids of the red pulp to the peritrabecular sinuses to the subcapsular sinuses to the splenic vein. No significant structural differences related to age were found.

show abstract

“…LSCM permits the examination of thicker slides than the ordinary LM does (Paddock 2000;Jirkovská et al 2005). Satoh et al (1997) used LSCM for recognition of white pulp stroma.…”

Section: Discussionmentioning

confidence: 99%

Some possibilities of representing microcirculation in human spleen

et al. 2009

View full text Add to dashboard Cite

The representation of microcirculation can be approached in several ways. One of the possibilities is to represent the endothelium (endothelial or sinus lining cells) and their basement membrane on the basis of detecting the known components and the expression of the surface antigenes by the methods of immuno-, enzyme-or lectino-histochemical analysis, or by staining or impregnation histological methods. The other possibility is the examination of samples by transmission and scanning electron microscopy. For three-dimensional demonstration corrosion casts techniques or laser scanning confocal microscopy can be used. In this paper we describe the survey of immuno-, enzyme-and lectino-histochemical characteristics of selected components of microcirculation and our own results of its demonstration in human spleen.

show abstract

Immunohistochemical and structural characteristics of the reticular framework of the white pulp and marginal zone in the human spleen

Abstract: The reticular framework of the PALS, LF, and MZ is specialized into heterogeneous components in the human spleen. The heterogeneity of the framework may induce the segregation of T and B lymphocytes.

Cited by 31 publications

References 30 publications

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

The spleen of the one humped camel (Camelus dromedarius) has a unique histological structure

Some possibilities of representing microcirculation in human spleen

Contact Info

Product

Resources

About