Immunohistochemical assessment of E-cadherin and β-catenin in trichofolliculomas and trichoepitheliomas

Bezděková, Michala; Brychtová, Světlana; Sedláková, Eva; Steigerova, Jana; Hlobílková, Alice; Bienová, Martina; Kučerová, Renata; Brychta, Tomáš; Krejčí, Veronika; Kolář, Zdeněk

doi:10.5507/bp.2007.042

Cited by 8 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…No correlation could be detected between the methylation status of APC or SFRP5 and the presence or localization of β-catenin. As it is known that the E-cadherin/β-catenin complex is important in both cell adhesion and canonical WNT signaling [46], we additionally analyzed the expression of E-cadherin, which is normally expressed throughout all layers of the epidermis, including hair follicles [47]. Expression of E-cadherin in 59 BCC was significantly lower compared with the normal epidermis ( P = 0.001), but was rated high (94.1%) in the tumor cells.…”

Section: Resultsmentioning

confidence: 99%

Epigenetic Changes in Basal Cell Carcinoma Affect SHH and WNT Signaling Components

et al. 2012

View full text Add to dashboard Cite

BackgroundThe genetic background of Basal Cell Carcinoma (BCC) has been studied extensively, while its epigenetic makeup has received comparatively little attention. Epigenetic alterations such as promoter hypermethylation silence tumor suppressor genes (TSG) in several malignancies.ObjectiveWe sought to analyze the promoter methylation status of ten putative (tumor suppressor) genes that are associated with Sonic Hedgehog (SHH), WNT signaling and (hair follicle) tumors in a large series of 112 BCC and 124 healthy control samples by methylation-specific PCR.ResultsGene promoters of SHH (P = 0.016), adenomatous polyposis coli (APC) (P = 0.003), secreted frizzled-related protein 5 (SFRP5) (P = 0.004) and Ras association domain family 1A (RASSF1A) (P = 0.023) showed significantly more methylation in BCC versus normal skin. mRNA levels of these four genes were reduced for APC and SFRP5 in BCC (n = 6) vs normal skin (n = 6). Down regulation of SHH, APC and RASSF1A could be confirmed on protein level as well (P<0.001 for all genes) by immunohistochemical staining. Increased canonical WNT activity was visualized by β-catenin staining, showing nuclear β-catenin in only 28/101 (27.7%) of BCC. Absence of nuclear β-catenin in some samples may be due to high levels of membranous E-cadherin (in 94.1% of the samples).ConclusionsWe provide evidence that promoter hypermethylation of key players within the SHH and WNT pathways is frequent in BCC, consistent with their known constitutive activation in BCC. Epigenetic gene silencing putatively contributes to BCC tumorigenesis, indicating new venues for treatment.

show abstract

Section: Resultsmentioning

confidence: 99%

Epigenetic Changes in Basal Cell Carcinoma Affect SHH and WNT Signaling Components

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Bongiovanni et al showed both canine epidermal and follicular tumors had a pattern of β-catenin expression similar to that reported in human counterparts [5,[11][12][13] . Studies in human cutaneous follicular tumours showed that mutations in β-catenin gene related to their tumorigenesis [7,11,14,15] .…”

Section: Discussionmentioning

confidence: 50%

“…They occur anywhere on the body with a slight preference for the back. It is an uncommon neoplasm in both human and domestic animal beings [1][2][3][4][5][6][7][8][9] .…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that differentiation of the hair follicle is regulated and linked by β-catenin that initiate its development [5] . With immunohistochemical studies, membrane labeling of β-catenin identified in adult inter follicular epidermis (IFE), outer root sheath (ORS), inner root sheath (IRS) of hair follicles and also in the epithelial cells of adnexal glands [6,7] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Occurrence of trichoepithelioma in a cat: Histopathologic and immunohistochemical study

Tavasoli

Golshahi

Rad

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

“…The reaction was visualized with the standard 3, 3 diaminobenzidine (DAB). Sections were counterstained with haematoxylin to facilitate detection brown color of E-cadherin expression [17] and examined then photographed using a light microscope (BX51, Olympus Optical Corporation, Tokyo, Japan) fitted with an Olympus digital camera (DP20).…”

Section: Immuno Histochemistry For E-cadherinmentioning

confidence: 99%

Restoration of Vaginal Epithelial Atrophy in Ovariectomized Rats with Sex Steroid Hormones

Abdel-Aal

Abdel-Hamid

Selim

2015

MOJAP

View full text Add to dashboard Cite

Histological and immunohistochemical changes in vaginal tissue structure of ovariectomized rats were assessed in response to sex steroid hormone administration. Sixty female rats were divided into six groups (10) each; control, sham, ovariectomized, ovariectomized rats treated with estradiol (20 µg/kg/day), Ovariectomized rats treated with progesterone (1 mg/kg/day) and ovariectomized rats treated with both estradiol and progesterone. Vaginal tissues were examined through haematoxylin and eosin, masson trichrome, and periodic acid shiff stains. Immunohistochemistry was used for expression of E-cadherin in vaginal epithelial cells. Ovariectomized rats with or without progesterone administration revealed a significant decrease in vaginal weight and length, reduction of vaginal epithelial thickness and glycogen content, which were restored with estradiol administration. Percent area of collagen fibers was not affected in all groups. E-cadherin expression was decreased in vaginal epithelium of both ovariectomized rats and progesterone administered group. It was concluded that vaginal epithelial atrophy due to ovariectomy can be restored by administration of estradiol or in combination with progesterone.

show abstract

Immunohistochemical assessment of E-cadherin and β-catenin in trichofolliculomas and trichoepitheliomas

Cited by 8 publications

References 27 publications

Epigenetic Changes in Basal Cell Carcinoma Affect SHH and WNT Signaling Components

Epigenetic Changes in Basal Cell Carcinoma Affect SHH and WNT Signaling Components

Occurrence of trichoepithelioma in a cat: Histopathologic and immunohistochemical study

Restoration of Vaginal Epithelial Atrophy in Ovariectomized Rats with Sex Steroid Hormones

Contact Info

Product

Resources

About