Immunohistochemical assessment of Eph/ephrin expression in oral squamous cell carcinoma and precursor lesions

Saito, Hiroki; Oikawa, Masahiro; Kouketsu, Atsumu; Takahashi, Tsuneo; Kumamoto, Hiroyuki

doi:10.1007/s10266-019-00466-y

Cited by 3 publications

(3 citation statements)

References 26 publications

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“…Moreover, Ephs overexpression is correlated with tumor size, clinical stage, lymph invasion, recurrence and distant metastasis in OSCC. Therefore, Ephs also identified as independent poor prognostic factors in this cancer type and may be used as a prognostic marker for OSCC 133,134 …”

Section: Functional Regulatory Network Of Emt In Osccmentioning

confidence: 99%

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Ling

Cheng

Tao

2020

Intl Journal of Cancer

147

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Oral squamous cell carcinoma (OSCC) is the most common malignancy representing 90% of all forms of oral cancer worldwide. Although great efforts have been made in the past decades, the 5‐year survival rate of OSCC patients is no more than 60% due to tumor metastasis and subsequent recurrence. The metastasis from the primary site is due to a complex process known as epithelial‐to‐mesenchymal transition (EMT). During the EMT, epithelial cells gradually acquire the structural and functional characteristics of mesenchymal cells, leading to the upregulation of cell migration and the promotion of tumor cell dissemination. Therefore, EMT attracted broad attention due to its close relationship with cancer invasion and metastasis. Therefore, in the present review, an extensive description of the current research on OSCC and the role of EMT in this cancer type is provided, including diverse EMT markers, regulatory networks and crucial EMT‐inducing transcription factors in OSCC. Moreover, a brief summary was made regarding the current application of EMT‐correlated indexes in the prognostic analysis of OSCC patients, and the potential therapeutic approaches against OSCC and difficulties in the development of an effective anti‐EMT treatment are discussed. Our aim is to provide novel insights to develop new strategies to combat OSCC by targeting EMT.

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Section: Functional Regulatory Network Of Emt In Osccmentioning

confidence: 99%

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Ling

Cheng

Tao

2020

Intl Journal of Cancer

147

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“…This phenomenon was also observed in oral epithelial precursor lesions and OSCC where D2-40 + LEC and basal keratinocytes were observed. 27 Of note, the D2-40 LVD was significantly lower in normal oral mucosa than in high-grade epithelial dysplasia or OSCC and it was significantly higher in OSCC than in low-grade epithelial dysplasia. 27 A recent systematic review 28 examining the prognostic value of LV markers in tongue cancer described promising results for the D2-40 marker based on studies that found high numbers of D2-40 + vessels predicted low overall survival.…”

Section: Discussionmentioning

confidence: 83%

“…27 Of note, the D2-40 LVD was significantly lower in normal oral mucosa than in high-grade epithelial dysplasia or OSCC and it was significantly higher in OSCC than in low-grade epithelial dysplasia. 27 A recent systematic review 28 examining the prognostic value of LV markers in tongue cancer described promising results for the D2-40 marker based on studies that found high numbers of D2-40 + vessels predicted low overall survival. 29,30 Nevertheless, it still remains that more D2-40 studies with larger patient cohorts are needed before D2-40 status can be included in prognostic algorithms for OSCC.…”

Section: Discussionmentioning

confidence: 83%

Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer

Chutipongpisit

Parachuru

Friedlander

et al. 2021

Int J Experimental Path

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Lymphangiogenesis makes an important contribution to the tumour microenvironment (TME), but little is known about this in oral squamous cell carcinoma (OSCC). Archival formalin‐fixed paraffin‐embedded specimens (28 OSCC, 10 inflamed and 6 normal oral mucosa controls) were processed using immunohistochemistry (IHC) with antibodies against lymphatic markers D2‐40 (podoplanin), LYVE‐1, VEGFR3 and Prox1. After the endothelial cells had been highlighted by the various markers for lymphatic endothelium, the positive stained cells and vessels were identified and counted in a systematic manner to determine microvessel density. Double‐labelling immunofluorescence (DLIF) was used to investigate the specificity of D2‐40 and LYVE‐1 to lymphatic endothelial cells (LECs) as opposed to blood ECs. There was higher D2‐40 and Prox1 lymphatic vessel density (P = .001) in the OSCC group when compared with both control groups. Some malignant keratinocytes expressed lymphatic markers, as did a much smaller number of epithelial cells in the control groups. DLIF showed that no vessels co‐expressed D2‐40/CD34 or LYVE/CD34. Some D2/40+ LVs were LYVE−. D2‐40 was the most specific LEC marker in OSCC tissues. These results establish that the OSCC TME contains significantly more lymphatic vessels expressing D2‐40 and Prox1 than the control groups, which may play a role in facilitating lymphatic invasion and metastases.

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Molecular Crosstalk between the Immunological Mechanism of the Tumor Microenvironment and Epithelial–Mesenchymal Transition in Oral Cancer

et al. 2022

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Oral cancer is a significant non-communicable disease affecting both emergent nations and developed countries. Squamous cell carcinoma of the head and neck represent the eight major familiar cancer types worldwide, accounting for more than 350,000 established cases every year. Oral cancer is one of the most exigent tumors to control and treat. The survival rate of oral cancer is poor due to local invasion along with recurrent lymph node metastasis. The tumor microenvironment contains a different population of cells, such as fibroblasts associated with cancer, immune-infiltrating cells, and other extracellular matrix non-components. Metastasis in a primary site is mainly due to multifaceted progression known as epithelial-to-mesenchymal transition (EMT). For the period of EMT, epithelial cells acquire mesenchymal cell functional and structural characteristics, which lead to cell migration enhancement and promotion of the dissemination of tumor cells. The present review links the tumor microenvironment and the role of EMT in inflammation, transcriptional factors, receptor involvement, microRNA, and other signaling events. It would, in turn, help to better understand the mechanism behind the tumor microenvironment and EMT during oral cancer.

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Immunohistochemical assessment of Eph/ephrin expression in oral squamous cell carcinoma and precursor lesions

Cited by 3 publications

References 26 publications

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer

Molecular Crosstalk between the Immunological Mechanism of the Tumor Microenvironment and Epithelial–Mesenchymal Transition in Oral Cancer

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