Immunohistochemical Assessment of Estrogen Receptor Distribution in the Human Endometrium throughout the Menstrual Cycle

Press, Michael F.; Nousek-Goebl, Nancy A.; King, William J.; Herbst, Arthur L.; Greene, Geoffrey L.

doi:10.1097/00006254-198510000-00017

Cited by 40 publications

(64 citation statements)

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“…These results are in strong contrast to the results published by others (Pickartz et al 1990;Press et al 1984;Press et al 1986), who, also using antibodies specific to the estrogen receptor, detected persistence of the estrogen receptor in the zona basalis during the whole luteal phase, despite a dramatic reduction in the number of estrogen receptors in the zona functionalis. Press et al (1984) found up to 25% strongly estrogen receptorpositive nuclei in the zona basalis (compared to approximately 3% in the functionalis) during the mid-and late luteal phase in the human endometrium.…”

Section: Comparisons To the Human Endometriumcontrasting

confidence: 99%

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The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

Esch¹,

Cline²,

Buse³

et al. 2008

Toxicol Pathol

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The macaque endometrium undergoes dramatic morphologic and functional changes during the menstrual cycle that are nearly identical to those of the human endometrium. The sequential events that take place in the endometrium are mainly driven by the ovarian steroids and their respective receptors. To be able to interpret the changes and effects induced by mammalian or synthetic hormones and other compounds that could have influence on the hormonal status of the animal, a thorough knowledge of the anatomy, physiology, and histology of the cyclic hormone-mediated processes within the endometrium is indispensable. In this paper we give an overview of uterine growth and development, anatomy, basic histology, aging, spontaneous pathology, and the techniques to study the endometrium in-life. In addtion, a comprehensive description of the receptor-mediated, hormonedriven morphological changes during the menstrual cycle in the cynomolgus monkey (Macaca fascicularis) is given. Where possible, differences between the macaque and human endometria are discussed.Keywords: female reproduction; endometrium; histology; menstrual cycle; hormone receptors; endometrial dating; cynomolgus monkey.human. For this reason, monkey species belonging to this group are preferred as experimental models in studies of human reproduction, pharmacology, and toxicology. Especially for studies with sex hormones, synthetic steroidal compounds or compounds that interfere with the hypothalamus-pituitary-ovarian (or testicular) axis, such monkey species are predictive . The rhesus monkey (Macaca mulatta) and the cynomolgus monkey (Macaca fascicularis) are the species most often used for these purposes. In our laboratories, purpose-bred cynomolgus monkeys are used for such studies. Besides their resemblance in menstrual cycle physiology, these monkeys are relatively small and easy to handle.Both the human and the macaque monkey endometria undergo dramatic morphologic and functional changes during the menstrual cycle. The sequential events that take place in the endometrium are mainly driven by the ovarian steroids estradiol and progesterone and their respective receptors.To be able to interpret the changes and effects induced by mammalian or synthetic hormones and other compounds that could have influence on the hormonal status of the animal, or that interfere with the regulatory processes within the endometrium, a thorough knowledge of the anatomy, physiology, and histology of cyclic hormone-mediated processes within the endometrium is indispensable.In this paper we give a brief overview of endometrial growth and development, anatomy, basic histology, and the effects of aging, and we briefly discuss the spontaneous pathology of the endometrium of macaques. The receptor-mediated,

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Section: Comparisons To the Human Endometriumcontrasting

confidence: 99%

“…Within a rejuvenated endometrium, progesterone receptors also can be reinduced, and in principle, a normal cyclic endometrium can be reestablished. After a prolonged period of destimulation, however, the estrogen receptors vanish (Press et al 1984).…”

Section: Endometrial Agingmentioning

confidence: 99%

The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

Esch¹,

Cline²,

Buse³

et al. 2008

Toxicol Pathol

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“…The decrease in OR positive cases in the second half of the menstrual cycle is the same as the endometrium (Press et al, 1984;Lessey et al, 1988) and is consistent with the accepted dogma of OR down-regulation as a consequence of progesterone action. The present study further supports this from observations in OC cycle, where there is a striking decrease in detection with use, presumably on account of downregulation by exogenous progestins.…”

Section: Discussionsupporting

confidence: 86%

“…What is clear from our data is that neither endogenous (natural) or exogenous (OC) progestins dramatically down-regulate breast epithelial PR. This contrasts with endometrial receptors (Press et al, 1988) and thus provides another example in which these two types of epithelium differ.…”

Section: Discussionmentioning

confidence: 90%

Influence of menstrual cycle, parity and oral contraceptive use on steroid hormone receptors in normal breast

Battersby

Robertson²,

Anderson³

et al. 1992

Br J Cancer

112

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Summary Steroid receptor was assessed immunohistochemically in 158 samples of normal breast for variation through the menstrual cycle. Patterns and intensity of reaction were used in a semi-quantitative scoring system to examine the influence of cycle phase, cycle type, parity and age. The changes in oestrogen receptor for natural cycle and oral contraceptive (OC) cycles indicated down-regulation by progestins. Progesterone receptor did not vary significantly in natural cycles, but increased steadily through OC cycles. This study provides strong evidence that both oestrogen and progesterone influence breast epithelium, but dissimilarities from the endometrium are apparent. The interval since pregnancy had a significant negative effect on frequency and score of oestrogen receptor and score of progesterone receptor. Multivariate analysis established the phase of cycle and OC use as independent significant influences on oestrogen receptor. The interval since pregnancy was an independent significant factor for both oestrogen and progesterone receptor presence.

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“…Estrogen receptor was detected in the nuclei of the vast majority of epithelial and stromal cells from early, middle, and late proliferative phase of endometrium. Middle and late secretory endometrium phase showed a dramatic reduction in the amount of localized estrogen receptors (3).…”

Section: Discussionmentioning

confidence: 98%

Estrogen and Progesterone Receptors in Endometrium in Women with Unexplained Infertility

Godinjak¹,

Bilalović²

2014

Mater Sociomed

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Aim:The purpose of the present study is to evaluate the relationship between endometrial concentrations of estrogen and progesterone receptors throughout the menstrual cycle in women with unexplained infertility. Material and methods: In forty four infertile women with unexplained infertility, biopsy of the endometrium was performed during simultaneous laparoscopy and hysteroscopy. Material was prepared for immunohistochemical staining. Forty four endometrial samples obtained from women with normal menstrual cycles were divided into four categories: early proliferative, late proliferative, early secretory and late secretory. Immunohistochemical localization of estrogen receptors (ER) and progesterone receptors (PR) was scored according to intensity of staining and proportion of cells specifically stained in glandular epithelium and stroma and results were analyzed. Conclusion: The early secretory phase appeared to be period of transition from the strong and ubiquitous staining for receptor characteristic of proliferative phase of endometrium to the weak, focal pattern of estrogen receptors. Progesterone receptors in early secretory phase were of strong staining and sufficient number of stained cells.

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Immunohistochemical Assessment of Estrogen Receptor Distribution in the Human Endometrium throughout the Menstrual Cycle

Cited by 40 publications

References 0 publications

The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

Influence of menstrual cycle, parity and oral contraceptive use on steroid hormone receptors in normal breast

Estrogen and Progesterone Receptors in Endometrium in Women with Unexplained Infertility

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