Immunohistochemical characterization of neurons and neuronal processes in the dorsal vagal nucleus of the pig

Gańko, Marta; Rychlik, Andrzej; Całka, Jarosław

doi:10.2478/pjvs-2013-0002

Cited by 6 publications

(3 citation statements)

References 50 publications

(67 reference statements)

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“…Identification of the SP NK 1 receptor on vagal efferent somata provides direct confirmation of this pathway (Plata-Salaman et al 1989; Le Brun et al 2008). Possible physiological implications of the SP, LENK, CART, GAL, NOS, and VIP immunopositive processes in the regulation of stomach function have been outlined in our former reports (Gańko and Całka 2013; Gańko et al 2013). …”

Section: Discussionsupporting

confidence: 56%

Prolonged Acetylsalicylic-Acid-Supplementation-Induced Gastritis Affects the Chemical Coding of the Stomach Innervating Vagal Efferent Neurons in the Porcine Dorsal Motor Vagal Nucleus (DMX)

Gańko

Całka

2014

J Mol Neurosci

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The main goal of our research was to study the possible alterations of the chemical coding of the dorsal motor vagal nucleus (DMX) neurons projecting to the porcine stomach prepyloric region following prolonged acetylsalicylic acid supplementation. Fast Blue (FB) was injected into the studied area of the stomach. Since the seventh day following the FB injection, acetylsalicylic acid (ASA) was given orally to the experimental gilts. All animals were euthanized on the 28th day after FB injection. Medulla oblongata sections were then processed for double-labeling immunofluorescence for choline acetyltransferase (ChAT), pituitary adenylate cyclase-activating peptide (PACAP), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), galanin (GAL), substance P (SP), leu enkephalin (LENK), and cocaine- and amphetamine-regulated transcript (CART). In the control DMX, only PACAP was observed in 30.08 ± 1.97 % of the FB-positive neurons, while VIP, NOS, GAL, SP, LENK, and CART were found exclusively in neuronal processes running between FB-labeled perikarya. In the ASA DMX, PACAP was revealed in 49.53 ± 5.73 % of traced vagal perikarya. Moreover, we found de novo expression of VIP in 40.32 ± 7.84 %, NOS in 25.02 ± 6.08 %, and GAL in 3.37 ± 0.85 % of the FB-labeled neurons. Our results suggest that neuronal PACAP, VIP, NOS, and GAL are mediators of neural response to aspirin-induced stomach inflammatory state.

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Section: Discussionsupporting

confidence: 56%

Prolonged Acetylsalicylic-Acid-Supplementation-Induced Gastritis Affects the Chemical Coding of the Stomach Innervating Vagal Efferent Neurons in the Porcine Dorsal Motor Vagal Nucleus (DMX)

Gańko

Całka

2014

J Mol Neurosci

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“…ChAT, as a primary motor fascicles marker, is widely reported to be highly expressed in human facial motor neurons and rat facial motor neurons [ 38 , 39 ], with high concentrations in motor neurons in both the central nervous system (CNS) and peripheral nerve axons [ 40 , 41 ]. A key feature of motor neuron development and function is the expression of the acetylcholine biosynthetic enzyme, ChAT [ 42 , 43 ]. Yuan et al used ChAT as an enzymatic marker in immunohistochemistry staining to detect motor axons and used NF200 to detect sensory axons and showed that the sciatic nerve contains both motor and sensory axons [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of sensory and motor nerve fascicles by immunofluorescence staining after peripheral nerve injury

Zhou

Qing

et al. 2021

J Transl Med

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Background Inappropriate matching of motor and sensory fibers after nerve repair or nerve grafting can lead to failure of nerve recovery. Identification of motor and sensory fibers is important for the development of new approaches that facilitate neural regeneration and the next generation of nerve signal-controlled neuro-prosthetic limbs with sensory feedback technology. Only a few methods have been reported to differentiate sensory and motor nerve fascicles, and the reliability of these techniques is unknown. Immunofluorescence staining is one of the most commonly used methods to distinguish sensory and motor nerve fibers, however, its accuracy remains unknown. Methods In this study, we aim to determine the efficacy of popular immunofluorescence markers for motor and sensory nerve fibers. We harvested the facial (primarily motor fascicles) and sural (primarily sensory fascicles) nerves in rats, and examined the immunofluorescent staining expressions of motor markers (choline acetyltransferase (ChAT), tyrosine kinase (TrkA)), and sensory markers [neurofilament protein 200 kDa (NF-200), calcitonin gene-related peptide (CGRP) and Transient receptor potential vanillic acid subtype 1 (TRPV1)]. Three methods, including the average area percentage, the mean gray value, and the axon count, were used to quantify the positive expression of nerve markers in the immunofluorescence images. Results Our results suggest the mean gray value method is the most reliable method. The mean gray value of immunofluorescence in ChAT (63.0 ± 0.76%) and TRKA (47.6 ± 0.43%) on the motor fascicles was significantly higher than that on the sensory fascicles (ChAT: 49.2 ± 0.72%, P < 0.001; and TRKA: 29.1 ± 0.85%, P < 0.001). Additionally, the mean gray values of TRPV1 (51.5 ± 0.83%), NF-200 (61.5 ± 0.62%) and CGRP (37.7 ± 1.22%) on the motor fascicles were significantly lower than that on the sensory fascicles respectively (71.9 ± 2.32%, 69.3 ± 0.46%, and 54.3 ± 1.04%) (P < 0.001). The most accurate cutpoint occurred using CHAT/CRCP ratio, where a value of 0.855 had 100% sensitivity and 100% specificity to identify motor and sensory nerve with an area under the ROC curve of 1.000 (P < 0.001). Conclusions A combination of ChAT and CGRP is suggested to distinguish motor and sensory nerve fibers.

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“…Neurons localized in the DMV, project to the upper gastrointestinal tract including the liver, whereas those located in the NA project to the esophagus and the cardiorespiratory system. Despite integration of peripheral and central signals concerning gastrointestinal processes, the preganglionic parasympathetic cells of the DMV also directly influence gastrointestinal activities (12).…”

Section: Ans In Control Of Glucose Availability By Central Action Of Insulinmentioning

confidence: 99%

The role of insulin at brain-liver axis in the control of glucose production

Ribeiro

Antunes

2018

American Journal of Physiology-Gastrointestinal and Liver Physi

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Glucose is an essential metabolic substrate for all mammalian cells, and its availability in the circulation is carefully controlled to avoid wide variations. Different mechanisms are involved in the glucose disposal, such as an adequate pancreatic and hepatic function. Insulin is the main hormone in glycemic control, and its action occurs directly in the cells, as well as in the liver, in an indirect way, to ultimately control the glycemia. Insulin has also an important action within the central nervous system, more precisely in the hypothalamus that projects directly to preautonomic nuclei in the brain stem to control hepatic glucose production. The central action of insulin relies on autonomic outflow through the vagal innervation of the liver, where insulin is able to modulate the production of glucose at this organ level. In this way, responses generated in the CNS reach the effector organs by autonomic efferent pathways as part of an important brain-organ axis in the control of glycemia. The purpose of this minireview is to shed light on the brain-liver axis in the control of hepatic glucose by central action of insulin via the autonomic nervous system.

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Immunohistochemical characterization of neurons and neuronal processes in the dorsal vagal nucleus of the pig

Cited by 6 publications

References 50 publications

Prolonged Acetylsalicylic-Acid-Supplementation-Induced Gastritis Affects the Chemical Coding of the Stomach Innervating Vagal Efferent Neurons in the Porcine Dorsal Motor Vagal Nucleus (DMX)

Prolonged Acetylsalicylic-Acid-Supplementation-Induced Gastritis Affects the Chemical Coding of the Stomach Innervating Vagal Efferent Neurons in the Porcine Dorsal Motor Vagal Nucleus (DMX)

Identification of sensory and motor nerve fascicles by immunofluorescence staining after peripheral nerve injury

The role of insulin at brain-liver axis in the control of glucose production

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