Immunohistochemical characterization of the regenerative compartment in biliary atresia: a comparison between Kasai procedure and transplant cases

Increased expression of senescence‐associated cell cycle regulators in the progression of biliary atresia: an immunohistochemical study

“…The coexpression of NCAM and senescence‐associated cell cycle regulators in small bile ducts and ductular cells in DR in biliary atresia is similar to what we have observed previously in PBC . We have also demonstrated the expression of NCAM in the ductular cells in DR in biliary atresia in our previous study . It is well known that NCAM is expressed in ductular cells in DR, and in several studies it is regarded as a marker of hepatic stem/progenitor cells (HSPCs) .…”

“…22 We have also demonstrated the expression of NCAM in the ductular cells in DR in biliary atresia in our previous study. 28 It is well known that NCAM is expressed in ductular cells in DR, 9 and in several studies it is regarded as a marker of hepatic stem/progenitor cells (HSPCs). [37][38][39] However, we have shown previously that NCAM-positive cells are observed mainly at a stage of disease when senescence-associated markers (p16 INK4a and p21 WAF1/Cip1 ) and the cell cycle G1phase marker (cyclin D) are also expressed, rather than while the cells were still proliferating.…”

“…Histological analysis was performed in a blinded manner by an experienced hepatopathologist (M.S.). The degree of DR was scored semiquantitatively as 0 (negative), 1 (<30% circumference in portal tracts), 2 (≥30% to <60% circumference in portal tracts) and 3 (≥60% circumference in portal tracts), as described previously . The degree of cholestasis was scored semiquantitatively as 0 (negative), 1 (mild), 2 (moderate) and 3 (extensive).…”

“…The degree of DR was scored semiquantitatively as 0 (negative), 1 (<30% circumference in portal tracts), 2 (≥30% to <60% circumference in portal tracts) and 3 (≥60% circumference in portal tracts), as described previously. 28 The degree of cholestasis was scored semiquantitatively as 0 (negative), 1 (mild), 2 (moderate) and 3 (extensive). The degree of giant cell change and bile duct loss was scored semiquantitatively as 0 (negative), 1 (mild) and 2 (extensive), respectively.…”

Immunohistochemical Analysis of the Stem Cell Marker Lgr5 in Pediatric Liver Disease

“…Recently, Kou et al showed by immunohistochemistry that the regenerative compartment is expanded in patients undergoing liver transplantation for BA, compared to patients with an earlier stage of disease undergoing Kasai hepatoportoenterostomy (31). Specifically, they found an increase in the biliary markers CK7, CK19, and CD56 in ductular reactions at time of transplant, which also corroborates that children with advanced disease recruit more HPCs from the biliary compartment (31). Collectively, these observations support the immunophenotypic heterogeneity of HPCs in relation to disease progression and severity.…”