Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells

Yamamoto, Yoshio; Hatakeyama, Taku; Taniguchi, Kazuyuki

doi:10.1016/j.neulet.2009.02.069

Cited by 60 publications

(57 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although our report is the first to demonstrate TRAAK expression in the nodose ganglia, TRAAK has been characterized by immunohistochemistry in the trigeminal ganglia (53). Yamamoto et al (53) reported that 60% of the trigeminal neurons are positive for TRAAK, whereas we find TRAAK expression in 30% of nodose neurons overall, increasing to 50% among gastrointestinal innervating neurons. We find that 47% of TRPV1 expressing neurons also express TRAAK, similar to the 41% reported by Yamamoto et al In contrast, we found that almost all TRAAK-positive neurons to also express TRPV1 (86%), whereas Yamamoto reported that 53% of TRAAK-positive neurons did not express TRPV1.…”

Section: Discussioncontrasting

confidence: 79%

Expression of transient receptor potential channels and two-pore potassium channels in subtypes of vagal afferent neurons in rat

Zhao

Sprunger

Simasko

2010

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

Vagal afferent neurons relay important information regarding the control of the gastrointestinal system. However, the ionic mechanisms that underlie vagal activation induced by sensory inputs are not completely understood. We postulate that transient receptor potential (TRP) channels and/or two-pore potassium (K2p) channels are targets for activating vagal afferents. In this study we explored the distribution of these channels in vagal afferents by quantitative PCR after a capsaicin treatment to eliminate capsaicin-sensitive neurons, and by single-cell PCR measurements in vagal afferent neurons cultured after retrograde labeling from the stomach or duodenum. We found that TRPC1/3/5/6, TRPV1-4, TRPM8, TRPA1, TWIK2, TRAAK, TREK1, and TASK1/2 were all present in rat nodose ganglia. Both lesion results and single-cell PCR results suggested that TRPA1 and TRPC1 were preferentially expressed in neurons that were either capsaicin sensitive or TRPV1 positive. Expression of TRPM8 varied dynamically after various manipulations, which perhaps explains the disparate results obtained by different investigators. Last, we also examined ion channel distribution with the A-type CCK receptor (CCK-R(A)) and found there was a significant preference for neurons that express TRAAK to also express CCK-R(A), especially in gut-innervating neurons. These findings, combined with findings from prior studies, demonstrated that background conductances such as TRPC1, TRPA1, and TRAAK are indeed differentially distributed in the nodose ganglia, and not only do they segregate with specific markers, but the degree of overlap is also dependent on the innervation target.

show abstract

Section: Discussioncontrasting

confidence: 79%

Expression of transient receptor potential channels and two-pore potassium channels in subtypes of vagal afferent neurons in rat

Zhao

Sprunger

Simasko

2010

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

show abstract

“…Cold-sensitive TREK potassium channels are found in up to thirty percent of TRPM8-expressing neurons, and the Kv1 family of potassium channels are also coexpressed to varying degrees [55,64]. Intriguingly, TRPM8 agonists have been shown to functionally affect a population of slowly adapting mechanoreceptors, although until molecular markers of mechanosensory phenotype are identified it is unknown what percentage of these neurons express TRPM8 [65].…”

Section: Neurochemical Phenotypementioning

confidence: 99%

TRPM8: From Cold to Cancer, Peppermint to Pain

Knowlton

McKemy²

2011

CPB

View full text Add to dashboard Cite

Temperature perception is vital for cellular and metabolic homeostasis, avoidance, and survival. In the primary afferent nerve terminal, select members of the transient receptor potential (TRP) family of ion channels reside and convert thermal stimuli into neuronal activity. The cold and menthol receptor, TRPM8, is the predominant thermoceptor for cellular and behavioral responses to cold temperatures. Remarkably, this single molecular sensor of cold, that responds at a discrete thermal threshold in vitro (approximately 28°C), enables sensory afferents to respond to distinct, yet varied thermal thresholds (approximately 28 to <5°C). Thus, unlike other thermally-gated TRP channels which are activated at either innocuous or noxious temperatures, TRPM8 provides perception of both pleasantly cool and painfully cold. In addition to this diversity in sensory signaling, TRPM8 has an emerging role in a variety of biological systems, including thermoregulation, cancer, bladder function, and asthma. Here we summarize some key points related to TRPM8 and its potential as a drug target to treat a wide variety of physiological conditions. Nonetheless, it remains to be seen how this single "cool" molecule can serve in such a multitude of biological processes.

show abstract

“…Accumulating evidence indicates that K2P channels are subject to modulation by inflammatory mediators; TREK-1 is inhibited by low extracellular pH and lysophosphatidic acid via stimulation of PLC and by PGE2 via cAMP signaling, which may contribute to increased excitability and inflammatory sensitization of peripheral nerve endings (Alloui et al 2006;Cohen et al 2009). Interestingly, both TREK-1 and TRAAK are highly co-expressed at the protein level with thermoTRP channels, including TRPM8 (Yamamoto et al 2009). However, behavioral investigation of TREK-1 and TRAAK null mutant mice revealed no change in cold sensitivity (Alloui et al 2006;Noel et al 2009).…”

Section: Other Thermo-sensitive Ion Channels That (May) Participate Imentioning

confidence: 99%

Ion channels involved in cold detection in mammals: TRP and non-TRP mechanisms

Babeș

2009

Biophys Rev

View full text Add to dashboard Cite

Substantial progress in understanding thermal transduction in peripheral sensory nerve endings was achieved with the recent cloning of six thermally gated ion channels from the TRP (transient receptor potential) super-family. Two of these channels, TRP melastatin 8 (TRPM8) and TRP ankyrin 1 (TRPA1), are expressed in dorsal root ganglion (DRG) and trigeminal ganglion (TG) neurons, are activated by various degrees of cooling, and are candidates for mediating gentle cooling and noxious cold, respectively. However, accumulating evidence suggests that more than just these two channels are involved in cold sensing in mammals. A recent report described a critical role of the voltage-gated tetrodotoxin-resistant sodium channel Na v 1.8 in perceiving intense cold and noxious stimuli at cold temperatures. Other ion channels, such as two-pore domain background potassium channels (K2P), are known to be expressed in peripheral nerves, have pronounced temperature dependence, and may contribute to cold sensing and/or cold hypersensitivity in pain states. This article reviews the evidence supporting a role for each of these channels in cold transduction, focusing on their biophysical properties, expression pattern, and modulation by pro-inflammatory mediators.

show abstract

Immunohistochemical colocalization of TREK-1, TREK-2 and TRAAK with TRP channels in the trigeminal ganglion cells

Cited by 60 publications

References 15 publications

Expression of transient receptor potential channels and two-pore potassium channels in subtypes of vagal afferent neurons in rat

Expression of transient receptor potential channels and two-pore potassium channels in subtypes of vagal afferent neurons in rat

TRPM8: From Cold to Cancer, Peppermint to Pain

Ion channels involved in cold detection in mammals: TRP and non-TRP mechanisms

Contact Info

Product

Resources

About