Immunohistochemical demonstration of a CRF-associated neurophysin in the external zone of the rat median eminence

Watkins, W. B.; Schwabedal, P. E.; Böck, R.

doi:10.1007/bf00218928

Cited by 61 publications

(6 citation statements)

References 57 publications

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“…The predominant effect of AVP is one of syn ergism with CRF on the release of adrenocorticotropic hor mone (ACTH) from pituitary corticotropes [8,17]; it has been co-localized with CRF in neurosecretory vesicles in the median eminence [22] and, in the adrenalectomized rat, in the perikarya of the parvicellular cells in the paraventri-cular nucleus of the hypothalamus [11,13,23], Indeed, CRF itself was thought to be a neurophysin-associated com pound before its isolation in 1981 [21], We have therefore studied the ontogeny of AVP in addition to that of CRF, to determine whether the two are expressed in parallel over the course of development.…”

mentioning

confidence: 99%

Ontogeny of Corticotropin-Releasing Factor and Arginine Vasopressein in the Rat

Rundle

Funder²

1988

Neuroendocrinology

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The ontogeny in the rat hypothalamus of arginine vasopressin (AVP) and corticotropin-releasing factor (CRF) was studied to determine whether these two peptides develop in parallel, given the synergistic effect of AVP with CRF on adrenocorticotropic hormone (ACTH) release. Hypothalami and extrahypothalamic cerebrum of 15-day fetal -40-day postpartum rats were extracted in acetic acid for radioimmunoassay. Both AVP- and CRF-like immunoreactivity was detected in whole brain extracts of 15-day fetuses. Hypothalamic CRF levels were low during fetal life, fluctuated around the time of birth, and then progressively rose to 63% of adult levels by day 21 postpartum. Extrahypothalamic levels of brain CRF paralleled hypothalamic levels. Hypothalamic AVP levels began to rise at fetal day 19 in a stepwise manner, with the greatest rise in levels occurring after day 14 postpartum. At day 21 postpartum hypothalamic levels were only 17% of adult. Extrahypothalamic AVP levels initially paralleled those in the hypothalamus but reached a plateau after day 4 post-partum, and did not rise again until after day 21. Hypothalamic CRF and AVP thus do not develop strictly in parallel; the adult ratios of these peptides (∼1:40) were never seen during development, perhaps of significance in the observed blunting of the hypothalamic-pituitary-adrenal response to stress in infant rats.

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confidence: 99%

Ontogeny of Corticotropin-Releasing Factor and Arginine Vasopressein in the Rat

Rundle

Funder²

1988

Neuroendocrinology

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“…This illus¬ trates that the AVP involved in ACTH release may arise directly from the hypothalamus in true neurosecretory fashion, rather than originating indirectly from the posterior lobe (Porter, Eskay, Oliver et al 1978), thus substantiating its role as a true CRF. This idea is amply supported by the extent of VP and neurophysin immunostaining in the zona externa of adrenalectomized and dexamethasone-treated animals (Watkins, Schwabadel & Bock, 1974;Zimmerman, Stillman, Recht et al 1977 (Linton et al 1983). It is not surprising that the CRF RIA was unable to detect CRF-41-like immunoreactivity in the effluent as the sensitivity of the RIA developed against synthetic oCRF is 100 ng/1 and it detects a total of only 700 pg per hypothalamus.…”

Section: Discussionmentioning

confidence: 89%

Involvement of rat corticotrophin-releasing factor-41-related peptide and vasopressin in adrenocorticotrophin-releasing activity from superfused rat hypothalami in vitro

Gillies¹,

Puri²,

Hodgkinson³

et al. 1984

Journal of Endocrinology

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Superfused rat hypothalamic pieces stimulated with a high K+ medium released corticotrophin-releasing factor (CRF) as detected in the rat isolated anterior pituitary cell column bioassay. This activity has components related to ovine CRF-41 (oCRF-41) and vasopressin as assessed by the effects of specific antisera on the ACTH-releasing activity of the hypothalamic column effluent. Release of immunoactive vasopressin paralleled that of CRF bioactivity. Immunoactive oCRF-41 could not be detected, as it is likely to be below the sensitivity of present radioimmunoassays.

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“…Lesions of the paraventricular nuclei abolished this median eminence vasopressin, suggesting that vasopressin fibers in the median eminence originate from the paraventricular nuclei (215). Vasopressin and neurophysin in the median eminence increased markedly after adrenalectomy; this increase was reversed by treatment with glucocorticoids (215,217,218).…”

Section: Role Of Vasopressin and Related Peptides In Stimulating Acthmentioning

confidence: 92%