Immunohistochemical demonstration of amylase in normal and neoplastic salivary glands.

Hatakeyama, Setsuko; Sashima, Mieko; Tsushima, Toshio; Itagaki, Mitsunobu; Suzuki, Atsumi

doi:10.2330/joralbiosci1965.27.702

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Numerous studies have been carried out to localize the various proteins in different salivary glands (Tourville et al, 1969;Kraus and Mestecky, 1971;Klockars and Reitamo, 1975;Mason and Taylor, 1975;Reitamo et al, 1980;Korsrud and Brandtzaeg, 1982). a-Amylase comprises the major protein component of parotid gland, and there is substantial histochemical evidence to show that the enzyme is produced and secreted by the serous acinar cells (Vreugdenhil et al, 1982;Hatakeyama et al, 1985).…”

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confidence: 99%

Secretion of α‐amylase in human parotid gland epithelial cell culture

Chopra

Xue-Hu

1993

Journal Cellular Physiology

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The secretions of the salivary gland system are essential for the maintenance of oral health. The nature of cell-specific secretions of the various glands and their regulation is not completely understood. The objective of this study was to establish epithelial cell cultures from the human parotid gland that exhibit the tissue-specific function of alpha-amylase secretion. A specimen of normal human parotid gland was obtained at surgery and used to obtain primary cultures by the explant/outgrowth procedure. The cultures were maintained in keratinocyte basal medium, supplemented with insulin (5 micrograms/ml), EGF (10 ng/ml), hydrocortisone (0.5 micrograms/ml), bovine pituitary extract (25 micrograms/ml), and antibiotics. The cultures were passaged using 0.125% trypsin to dissociate the cells. Phase contrast and ultrastructural observations showed that the cells were polygonal and exhibited desmosomes. Their cytoplasm contained tonofilament bundles and abundant rough endoplasmic reticulum and Golgi complexes. Immunofluorescence studies showed that all cells were positive for cytokeratins. Immunoblot analysis revealed keratins with molecular weights of 58, 56, 52, 50, 48, 46, and 40 KD, which are characteristic of secretory epithelia. The cells have been passaged 35 times so far, undergoing a cumulative 120-140 population doublings. The serially passaged epithelial cell cultures produced and secreted alpha-amylase, a major component of parotid gland acinar cell secretion. The beta-adrenergic agonist, isoproterenol (ISP), stimulated alpha-amylase secretion, which was accompanied by increased intracellular concentrations of cAMP. ISP-induced stimulation of amylase and cAMP was blocked by the beta-adrenergic antagonist, propranolol. Further, dibutyryl cAMP also enhanced the secretion of amylase. Thus we have established a long-term epithelial cell culture model of human parotid gland epithelial cells that exhibits differentiated function and retains the intact beta-adrenergic receptor system.

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confidence: 99%

Secretion of α‐amylase in human parotid gland epithelial cell culture

Chopra

Xue-Hu

1993

Journal Cellular Physiology

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“…The Immunohistochemistry (IHC) staining for the alpha‐smooth‐muscle actin (alpha SMA) and salivary alpha‐amylase was done using the standard protocol to detect myoepithelial cells and salivary amylase in the walls of the seromucous acini of the examined tissues. Owing to the known presence of the myoepithelial cells (Balachander et al, 2015) and salivary amylase in the acini of the salivary glands (Hatakeyama et al, 1985), the submandibular gland was used as a positive control. The 5μ thick paraffin‐embedded tissue sections were mounted on the frosted slides coated with Poly‐ l ‐Lysine solution.…”

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confidence: 99%

Morpho‐functional characterization of the submucosal glands at the nasopharyngeal end of the auditory tube in humans

et al. 2022

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Background:The auditory tube (AT), an osteocartilaginous channel, connects the nasopharynx to the middle ear cavity. At the nasopharyngeal opening of the AT, there are dense collections of submucosal glands. In a recent article, Valstar et al. proposed these nasopharyngeal tubal glands conglomerate as salivary glands, which starkly contrasts with their previously known anatomy for being a component of the respiratory tract. This study examines the contesting views regarding the taxonomical categorization of the nasopharyngeal tubal glands. Materials and Methods:The AT glands in context were examined in human cadavers grossly, and microscopically using routine and special (Hematoxylin and Eosin [H&E] and Periodic acid-Schiff [PAS] respectively), as well as immunohistochemical (for alpha-SMA and salivary amylase) staining methods and compared with the major and minor salivary glands and the submucosal glands in the trachea. Further, a biochemical analysis was performed to detect the presence of salivary amylase in the oral and nasopharyngeal secretions of the four living human subjects, representing major salivary glands and tubal glands, respectively. Results:The submucosal seromucous glands with a surface lining of respiratory epithelium were observed at the nasopharyngeal end of AT. The cells in the tubal glands showed cytoplasmic positivity for alpha-SMA, which indicated the presence of the myoepithelial cells; however, this expression was significantly lower than in the seromucous submucosal glands within the trachea. Salivary alpha-amylase was undetectable in the cadaveric tissue samples. Moreover, the amylase level in the nasopharyngeal swabs was negligible compared to the oral swabs. Conclusion:The anatomical location along the respiratory tract, the presence of respiratory epithelium in the overlying mucosa, their morpho-functional resemblance to the seromucous glands in the trachea, and the absence of salivary amylase strongly indicate that the tubal glands are taxonomically different from the salivary glands.Given the available evidence, their existing recognition as a part of the respiratory tract and an integral component of the AT seems more appropriate.

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A feasibility study of salivary gland autograft transplantation for xerostomia

2000

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Background Radiation‐induced xerostomia is a frequent sequela in patients treated for cancer of the head and neck. One strategy to treat xerostomia would be to relocate portions of salivary tissue to adjacent submucosal sites that lie outside the radiation portals such as the anterior oral vestibule. It is not known whether salivary tissue transplanted as an autogenous free graft can survive, function adequately, and not produce mucoceles. Methods Salivary gland tissue from the parotid and submandibular glands of the Syrian hamster were transplanted into the submucosal layer of the cheek pouch. After 3 months of observation, looking at graft size, graft extrusion, ulceration, infection, and mucocele formation, the graft sites were harvested. The specimens then underwent pathologic analysis by hematoxylin and eosin staining, as well as immunohistochemical methods to determine positivity for cytokeratin, smooth muscle actin (SMA), and amylase. Results Histologic analysis of tissue harvested from Syrian hamsters grafted into the cheek pouch demonstrated intact, viable, organized salivary gland tissue. Eighty percent of the animals in the submandibular group and 63% of the animals in the parotid group had at least 1 graft with viable salivary tissue without undue complications. Conclusions Salivary gland tissue can be transplanted successfully as free autogenous grafts in the Syrian hamster model. Further studies are needed to determine whether the grafts will subsequently become functional and whether growth can be biologically stimulated. This approach may be a useful strategy to protect salivary gland tissue in patients undergoing radiotherapy for head and neck cancer. © 2000 John Wiley & Sons, Inc. Head Neck 22: 241–246, 2000.

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Immunohistochemical demonstration of amylase in normal and neoplastic salivary glands.

Cited by 5 publications

References 12 publications

Secretion of α‐amylase in human parotid gland epithelial cell culture

Secretion of α‐amylase in human parotid gland epithelial cell culture

Morpho‐functional characterization of the submucosal glands at the nasopharyngeal end of the auditory tube in humans

A feasibility study of salivary gland autograft transplantation for xerostomia

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