Immunohistochemical demonstration of contractile proteins in astrocytes, marginal glial and ependymal cells in rat diencephalon

Gröschel‐Stewart, Ute; Unsicker, Klaus; Leonhardt, H

doi:10.1007/bf00227035

Cited by 27 publications

(9 citation statements)

References 10 publications

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“…Most conspicuous in tanycytes, the protein is concentrated beneath the apical plasma membrane, where cortactin is also present. Moreover, immunoreactivity for actin is particularly strong in the apical cytoplasm of tanycytes and several ependymal cells (this study;and Gröschel-Stewart et al 1977).…”

Section: Discussionmentioning

confidence: 54%

The Cortactin-binding Postsynaptic Density Protein ProSAP1 in Non-neuronal Cells

Redecker

Gundelfinger²,

Boeckers³

2001

J Histochem Cytochem.

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(TMB) S U M M A R Y Proline-rich synapse-associated protein-1 (ProSAP1) is a neuronal PDZ domain-containing protein that has recently been identified as an essential element of the postsynaptic density. Via its interaction with the actin-binding protein cortactin and its integrative function in the organization of neurotransmitter receptors, ProSAP1 is believed to be involved in the linkage of the postsynaptic signaling machinery to the actin-based cytoskeleton, and may play a role in the cytoskeletal rearrangements that underlie synaptic plasticity. As a result of our ongoing studies on the distribution and function of this novel PDZ domain protein, we now report that the expression of ProSAP1 is restricted neither to neurons and interneuronal junctions nor to the nervous system. Using immunohistochemical techniques in conjunction with specific antibodies, we found that, in the CNS, ProSAP1 can be detected in certain glial cells, such as ependymal cells, tanycytes, subpial/radial astrocytes, and in the choroid plexus epithelium. Moreover, our immunohistochemical analyses revealed the presence of ProSAP1 in endocrine cells of the adenohypophysis and of the pancreas, as well as in non-neuronal cell types of other organs. In the pancreas, ProSAP1 immunoreactivity was also localized in the duct system of the exocrine parenchyma. Our findings demonstrate that, in addition to neurons, ProSAP1 is present in various non-neuronal cells, in which it may play a crucial role in the dynamics of the actin-based cytoskeleton.

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Section: Discussionmentioning

confidence: 54%

The Cortactin-binding Postsynaptic Density Protein ProSAP1 in Non-neuronal Cells

Redecker

Gundelfinger²,

Boeckers³

2001

J Histochem Cytochem.

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“…The expression of F-actin (Chiu et al, 1981;CiesielskiTreska et al, 1982;Kalnins et al, 1984), myosin (Groschel-Stewart et al, 1977;Braak et al, 1978), tropomyosin (Fedoroff et al,198l), and spectrin has been previously demonstrated in developing astroglia.…”

Section: Introductionmentioning

confidence: 93%

Actin and actin‐binding proteins in differentiating astroglia in tissue culture

Abd-El-Basset

Ahmed

Fedoroff

1991

J of Neuroscience Research

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In this paper we have described the organization of F-actin and actin-binding proteins (ABP): alpha-actinin, myosin, tropomyosin, caldesmon, vinculin, talin, and spectrin, in differentiating astroglia in colony cultures. We observed that the microfilament (MF) network arrangements differ at various stages of astroglia development, but the composition of MF bundles and stress fibers is the same at all developmental stages. F-actin is closely colocalized with myosin, tropomyosin, caldesmon, and alpha-actinin. The striated pattern of myosin, tropomyosin, and caldesmon are superimposable. Tropomyosin and caldesmon extend along F-actin but are interrupted for short periods, whereas myosin is interrupted for longer periods. alpha-actinin colocalizes with tropomyosin and caldesmon but not with myosin. In astroglia at different stages of development spectrin is arranged in the form of fine networks spreading through the cell and does not follow the arrangement of MF bundles. Only F-actin, alpha-actinin, and vinculin can be detected at cell-cell junctions. In the areas of the focal contacts, F-actin, alpha-actinin, vinculin, and talin are present. They overlap each other, although talin and vinculin extend toward the cell membrane beyond F-actin and alpha-actinin. Astroglia undergo well-defined states of nonmotility, motility, and nonmotility again during differentiation. The changes in motility are paralleled by changes in the organization of F-actin and ABP: as GFAP-containing intermediate filaments increase in differentiating astroglia, the F-actin and ABP are down-regulated, leading to non motility.

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“…Rat ependymal cells, and other epithelia at fluidtissue interfaces, show strong immunoreactivity for plectin, an intermediate filament associated protein (Errante et al, 1994). Rat ependymal cells also exhibit immunoreactivity for smooth muscle actin and myosin in the apical cytoplasm and striated muscle myosin near the basal bodies (Groschel-Stewart et al, 1977). These are probably related to the movement of cilia.…”

Section: Specialization General Morphology and Cytoskeletonmentioning

confidence: 99%

The ependyma: A protective barrier between brain and cerebrospinal fluid

Bigio

1995

Glia

289

182

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This review summarizes the current scientific literature concerning the ependymal lining of the cerebral ventricles of the brain with an emphasis on selective barrier function and protective roles for the common ependymal cell. Topics covered include the development, morphology, protein and enzyme expression including reactive changes, and pathology. Some cells lining the neural tube are committed at an early stage to becoming ependymal cells. They serve a secretory function and perhaps act as a cellular/axonal guidance system, particularly during fetal development. In the mature mammalian brain ependymal cells possess the structural and enzymatic characteristics necessary for scavenging and detoxifying a wide variety of substances in the CSF, thus forming a metabolic barrier at the brain-CSF interface.

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Immunohistochemical demonstration of contractile proteins in astrocytes, marginal glial and ependymal cells in rat diencephalon

Cited by 27 publications

References 10 publications

The Cortactin-binding Postsynaptic Density Protein ProSAP1 in Non-neuronal Cells

The Cortactin-binding Postsynaptic Density Protein ProSAP1 in Non-neuronal Cells

Actin and actin‐binding proteins in differentiating astroglia in tissue culture

The ependyma: A protective barrier between brain and cerebrospinal fluid

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