Immunohistochemical demonstration of keratins in the cysts of thyroid glands, parathyroid glands, and C‐cell complexes of the dog

1995

J Histochem Cytochem.

The localization of vimentin, an intermediate filament protein, in the dog thyroid cells was examined by immunohistochemistry with a monoclonal antibody against vimentin and polyclonal anti-19S-thyroglobulin and anti-calcitonin antibodies. In the dog thyroid gland, C-cells are usually distributed as large cell groups in an interfollicular location. In addition, C-cell complexes that are special C-cell clusters associated with other epithelial elements and cysts are frequently located around the parathyroid gland IV. The C-cell complexes are remnants of embryonic ultimobranchial bodies and retain fetal characteristics for long periods. Follicular cells in various stages of differentiation are observed in the C-cell complexes and large C-cell groups of postnatal dogs: (a) cell masses not yet organized into follicles, (b) primitive and small follicles storing colloid, and (c) small follicles without colloid storage. The immature follicular cells in the C-cell complexes and large C-cell groups express immunoreactivity for vimentin in addition to 19S-thyroglobulin. When the follicular cells stored considerable amounts of colloid in the follicular lumina, vimentin immunoreactivity disappeared from them. Typical thyroid follicles showed no immunoreactivity for vimentin. In dog fetuses in which follicular cells were arranged in cell clusters or were vigorously forming follicles, no immunoreactivity for vimentin was observed in the thyroid parenchyma. These results indicate that vimentin is specifically expressed in the immature follicular cells derived from the ultimobranchial anlage. The vimentin filaments may participate in increased cellular activities in the cells, i.e., thyroglobulin synthesis and folliculogenesis.

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Co-expression of vimentin and 19S-thyroglobulin in follicular cells located in the C-cell complex of dog thyroid gland.

1995

J Histochem Cytochem.

“…The vimentin MAb and the polyclonal anti-GFAP antiserum were purchased from Dako (Carpinteria, CA); polyclonal anti-S-100 antiserum was from Milab (Malmo, Sweden); the polyclonal anti-epidermal keratin antiserum was produced in this laboratory. The preparation and characterization of anti-epidermal keratin antiserum have been previously described (Kameda et al, 1987(Kameda et al, ,1986. Preliminary trypsinization was done for the immunoreaction of antiepidermal keratin antiserum (Kameda et al, 1986).…”

Section: Methodsmentioning

confidence: 99%

Differential distribution of S-100 protein and vimentin in the hypophyseal pars tuberalis of the guinea pig.

1996

J Histochem Cytochem.

In the hypophyseal pars tuberalis of guinea pigs, I examined immunohistochemical localization and development of vimentin and S-100 protein in comparison with those of the pars distalis. In the pars distalis, almost all folliculostellate cells expressed intense immunoreactivity for vimentin. A subpopulation of vimentin-immunoreactive folliculostellate cells was also immunoreactive for S-100 protein. During fetal development, vimentin-immunoreactive cells appeared at mid-gestation in the pars distalis and became numerous at late stages, whereas only a few S-100 immunoreactive cells were observed even at late stages. In the pars tuberalis, a large number of vimentin-immunoreactive cells were distributed in the cranial region surrounding the median eminence and the dorsocaudal region surrounding the infundibular stalk. These cells, however, were sparse in the ventrocaudal region in continuity with the pars distalis. Conversely, dense distribution of S-100 immunoreactive cells was restricted in the ventrocaudal region. Vimentin-immunoreactive cells were elongated and were mostly distributed as solitary cells, whereas S-100 immunoreactive cells were gathered in large or small cell groups and frequently formed colloid-containing follicles. During fetal development, large cell groups immunoreactive for S-100 protein were detected at late stages in the ventrocaudal region. Therefore, in the pars tuberalis S-100 immunoreactive cells are distinct from the vimentin cells and do not correspond to the folliculostellate cells of the pars distalis.

“…These complexes usually consist of C cells at various stages of development and cystic structures of various sizes. The cysts immunostain with antiserum against 50,000‐ and 58,000‐dalton keratins extracted from canine foot‐sole epidermis (Kameda, ). The complexes are considered to represent persistent ultimobranchial bodies that have not fused, or that have fused only partially, with the thyroid.…”

Section: Morphogenesis Of Thyroid C Cellsmentioning

confidence: 99%

Cellular and molecular events on the development of mammalian thyroid C cells

2016

Developmental Dynamics

Self Cite

Thyroid C cells synthesize and secrete calcitonin, a serum calcium-lowering hormone. This review provides our current understanding of mammalian thyroid C cells from the molecular and morphological perspectives. Several transcription factors and signaling molecules involved in the development of C cells have been identified, and genes expressed in the pharyngeal pouch endoderm, neural crest-derived mesenchyme in the pharyngeal arches, and ultimobranchial body play critical roles for the development of C cells. It has been generally accepted, without much-supporting evidence, that mammalian C cells, as well as the avian cells, are derived from the neural crest. However, by fate mapping of neural crest cells in both Wnt1-Cre/ R26R and Connexin(Cxn)43-lacZ transgenic mice, we showed that neural crest cells colonize neither the fourth pharyngeal pouch nor the ultimobranchial body. E-cadherin, an epithelial cell marker, is expressed in thyroid C cells and their precursors, the fourth pharyngeal pouch and ultimobranchial body. Furthermore, E-cadherin is colocalized with calcitonin in C cells. Recently, lineage tracing in Sox17-2A-iCre/R26R mice has clarified that the pharyngeal endoderm-derived cells give rise to C cells. Together, these findings indicate that mouse thyroid C cells are endodermal in origin. Developmental Dynamics 245:323-341, 2016. V C 2015 Wiley Periodicals, Inc.