Immunohistochemical demonstration of prostaglandins in various tissues of the rat

Abstract

To demonstrate the tissue localization of prostaglandin (PG) E2, PGF2 alpha and 6-keto-PGF1 alpha (a stable metabolite of PGI2) various tissues, including decalcified periodontal tissue of 7-week-old male Wistar strain rats, were immunohistochemically examined using a streptavidin-biotin complex method. Besides tissue macrophages and endothelial cells in various tissues, hepatocytes, renal tubular cells, and parietal and chief cells in the gastric mucosa showed a positive reaction for the various PGs examined. PGs were demonstrated in the cytoplasm or in association with the cell membrane. We generally observed no difference between the localization patterns of PGE2-, PGF2 alpha-, and 6-keto-PGF1 alpha-positive cells in these tissues. However, in the periodontal ligament and alveolar bone, 6-keto-PGF1 alpha was localized in the cytoplasm of osteocytes, osteoblasts, cementocytes, and cementoblasts, while no reaction for PGE2 or PGF2 alpha was revealed in these cells. We demonstrated the immunohistochemical localization of PGs in various rat tissues including decalcified periodontal tissue and discuss the important roles of PGs in the modulation of their normal functions in these tissues.

In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases

“…Despite the biological importance of PGs, localization of native PGs to specific cells in tissues has not yet been possible, although methods using PGs labelled with biotins have helped in this regard 22 , 23 . As a result, PG distribution in tissues has been largely assumed to correspond to the regional expression of COX2 and/or downstream PG synthases.…”

Immunocytochemical demonstration of the secretory dynamics of zymogenic contents in rat gastric gland processed by high-pressure freezing/freeze substitution, with special references to phospholipase A2 and phospholipase Cγ1

“…In stomach, PGs are thought to play an important role in the maintenance and defense of the gastric mucosa through their inhibitory effect on gastric acid secretion and their cytoprotective effect (Russell 1986). Miyauchi et al (1996) reported the immunohistochemical localization of PGE 2 , PGF 2α , and 6-keto-PGF 1α in the cytoplasm of parietal cells, and proposed that the parietal cells were the main source of endogenous PGs in rat stomach. In the present study, some of the PLA 2 immunolabeling on mucinous contents was associated with the apical membranes (microvilli) of parietal cells.…”

TRPM2 Immunoreactivity Is Increased in Fibroblasts, but not Nerves, of Symptomatic Human Dental Pulp

“…Under normal conditions, the aforementioned cell types provide maintenance and structural support and function to re- spond to pulpal insults. However, under pathological situations, such as irreversible pulpitis, cells become stimulated to release a variety of molecules that serve as inflammatory mediators, such as bradykinin, prostaglandins, neuropeptides, and interleukins (21)(22)(23)(24)(25)(26), leading to the induction of inflammation and sensitization of primary afferent neurons (22). The mechanisms by which nonneural pulpal cells are activated to release these inflammatory mediators are unclear.…”